Epidermal RNA samples from FVB/N mice treated with PAH standards and environmental PAH mixtures were harvested and purified using Trizol extraction and RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5), indicating significant RNA degradation and the need to modify handling, collection, and processing of skin...
Polycyclic aromatic hydrocarbons (PAHs) are present in the environment as complex mixtures with components that have diverse carcinogenic potencies and mostly unknown interactive effects. Non-additive PAH interactions have been observed in regulation of cytochrome P450 (CYP) gene expression in the CYP1 family. To better understand and predict biological effects of...
We have previously shown that relative potency factors and DNA adduct measurements are inadequate for predicting carcinogenicity of certain polycyclic aromatic hydrocarbons (PAHs) and PAH mixtures, particularly those that function through alternate pathways or exhibit greater
promotional activity compared to benzo[a]pyrene (BaP). Therefore, we developed a pathway
based approach for...
FVB/N mice wild-type, heterozygous or null for Cyp 1b1 were used in a two-stage skin tumor study comparing PAH, benzo[a]pyrene (BaP), dibenzo[def,p]chrysene (DBC), and coal tar extract (CTE, SRM 1597a). Following 20 weeks of promotion with TPA the Cyp 1b1 null mice, initiated with DBC, exhibited reductions in incidence, multiplicity,...
The polycyclic aromatic hydrocarbon (PAH), benzo[a]pyrene (BaP), was compared to dibenzo[def,p]chrysene (DBC) and combinations of three environmental PAH mixtures (coal tar, diesel particulate and cigarette smoke condensate) using a two stage, FVB/N mouse skin tumor model. DBC (4 nmol) was most potent, reaching 100% tumor incidence with a shorter latency...
Dibenzo[def,p]chrysene (DBC) is a transplacental carcinogen in mice (15 mg/kg; gestation day (GD) 17). To mimic residual exposure throughout pregnancy, dams received 4 smaller doses of DBC (3.75 mg/kg) on GD 5, 9, 13 and 17. This regimen alleviated the previously established carcinogenic responses in the thymus, lung, and liver....
Librarians often lament the difficult nature of keeping up with new online tools and trends in the face of shrinking staff and increasing duties. When the writers of infodoodads began blogging in December 2006 they hoped to pool their collective intelligence and make keeping up a little bit easier. The...
Correction
10 Aug 2012: Klinck H, Mellinger DK, Klinck K, Bogue NM, Luby JC, et al. (2012) Correction: Near-Real-Time Acoustic Monitoring of Beaked Whales and Other Cetaceans Using a Seaglider™. PLOS ONE 7(8): 10.1371/annotation/57ad0b82-87c4-472d-b90b-b9c6f84947f8.
The way that an individual defines and understands the term “disability” has the potential to affect how they view and interact with people with disabilities. Previous research suggests that classroom interventions and frequent interactions with people who have disabilities can be effective in promoting the social model of disability and...
Magnetic materials can be used in modern soft robotics as a method for external stimulus actuation and motion control. By combining aspects of biology and mechanics, devices are fabricated to create a structure capable of complex movement. Applications that these devices are subject to can be broken down into four...
Netarts Bay is a shallow, temperate, tidal lagoon located on the northern coast of Oregon and site of the Whiskey Creek Shellfish Hatchery (WCSH). Data collected with an automated flow-through system installed at WCSH capable of high-resolution (1 Hz) pCO2 and hourly TCO2 measurements, with measurement uncertainties of <2.0% and...
The Internet of Things (IoT) paradigm brought an ever-increasing dependence on low-power devices to collect sensor data and transmit that information to the cloud, placing greater demand on connectivity and lifespan. In response, rapid worldwide innovation demonstrates the trade-offs in processing, communication, and energy consumption with diverse approaches to low-power...
Contemporary fire effects are raising concerns about the resistance and resilience of dry mixed-conifer forests to large wildfires. Fire refugia – unburned or low-severity patches within fire perimeters – are understudied components of post-fire mosaics that may be key drivers of forest recovery following high-severity fire. Little is known about...
The production of titanium dioxide (TiO2) nanoparticles has increased significantly in the past decades, which has generated concern due to their known toxicity in the environment. As a result of the production, use and disposal of productions containing TiO2, TiO2 nanoparticles are often received by wastewater treatment facilities. The adsorption...
Basaltic volcanism provides a window into the Earth’s mantle. Seeing through this window requires seeing through the processes of magma genesis and evolution that distort the view. Radiogenic isotope ratios, and to a lesser extent incompatible elements and stable isotope ratios, allow us to see through these distortions and infer...
English holly culture in Oregon has been threatened by the
development of red leaf spots which prevent sale of cut holly.
Chemical, mechanical and insect injury and boron deficiency have
been shown in the past to cause different types of red leaf spotting,
but the cause of holly scab, a...