Histones are presumed to be of chromosomal origin and provide
both structural and functional elements for chromatin. In like
manner the ribosomal proteins are of ribosomal origin and have
analogous roles. Considerable controversy over their cellular roles
exists because of the inability to compare directly both groups of
proteins. In...
This study investigated some functional and
biochemical characteristics of a bacteriolytic protein in
the salivary glands of adult Aedes aecupti. A method for
the isolation of this protein from other mosquito salivary
gland components is also described. Based on some of its
biochemical properties, this bacteriolytic protein can be
classified...
The role of viral proteins in the pathogenesis of infectious hematopoietic necrosis
virus (IHNV) was studied at the molecular level. The expression of the viral genes at the
protein and RNA level, and their cellular localization, were characterized to further our
understanding of viral pathogenesis. The pathogenic effect of individual...
Protein disulfide isomerase (PDI), a member of the thioredoxin superfamily, contains two domains with significant sequence homology to the active sites in thioredoxin. PDI facilitates the folding of nascent proteins in the endoplasmic reticulum (ER), binds hormones and Ca²⁺, catalyzes the glutathione dependent reduction of dehydroascorbate, serves as a major...
The objectives of this study were to evaluate the feeding value
and potential toxicity of single cell protein (SCP) when fed to
ruminants.
Three in vivo digestibility trials with lambs, one feedlot trial
with steers and one long term feeding trial of high levels of SCP with
sheep were conducted...
Escherichia coli 16S-like ribosomal RNAs from
eubacterial, archaebacterial and eukaryotic organisms have
several universally conserved sequence zones which are
proposed to be single-stranded. A clear function for all
of these regions has not yet been defined. To evaluate the
roles of these sequence zones in protein synthesis,
knowledge of their...
The acute phase response (APR) includes an early response of the innate immune
system to an inflammatory agent. Acute phase proteins (APP) are produced at
increased rates in the liver during this response. The APR has been well
characterized in mammals, but it is unclear to what degree teleosts mount...
These studies focused on maximizing the nutrition and safety of raw whole
muscle beef while minimizing quality and shelf-life alteration. The first study used
response surface methodology to predict fluid loss from injected beef strip loins and
steaks as influenced by levels of salt and sodium phosphates (SP) in the...
Streptococcus gordonii is a bacterial species that naturally colonizes the oral cavity of most healthy humans. It resides in the mouth as an adherent to dental surfaces and, with few exceptions, does not cause disease in individuals it inhabits. It possesses qualities that encourage its use as a vector to...
Mitochondria provide energy for biological cells to function, but this process is also a source of oxygen radicals that are capable of damaging nearby proteins. Mitochondrial protein damage can eventually lead to cell death, especially in the case of heart cells, where mitochondria are present in the highest concentrations. As...
Glutathione, a ubiquitous intracellular tripeptide, protects cells by reacting with electrophiles and radicals that can damage cellular macromolecules. However, glutathione conjugation is now recognized as one mechanism by which drugs and xenobiotics can also be converted to reactive intermediates having toxic consequences to cells. The halogenated alkane 1,2-dichloroethane forms S-(2-chloroethyl)...
Because of large declines in abundance of many Eastern Pacific rockfish populations (Genus Sebastes), there has been an increasing effort to improve our understanding of the role of spawning population characteristics and individual reproductive success in recruitment variability and population dynamics. Current methods for assessing sex and maturational status in...
Plants develop a vast array of cell shapes and sizes by selectively modifying their surrounding cell walls to expand in some regions and not in others. This process of morphogenesis requires the delivery of secretory vesicles to specific locations at the cell periphery, where exocytosis adds new membrane and proteins...
Chlamydiae are obligate intracellular bacteria that infect a variety of
eukaryotic hosts and affect normal host processes. Within host cells, their
developmental cycle takes place inside non-acidified vacuoles termed inclusions. An
inclusion membrane composed primarily of secreted chlamydial synthesized proteins
called Incs encloses the inclusion. At this location, Incs have...
The thesis begins with an introduction to signal transduction
and an analysis of current understanding of plant signal
transduction. There are similarities between plants and animals,
but also key differences, including lack of protein kinase C and of a
cAMP signaling pathway in plants, and presence in plants of
calcium...
This thesis involves analyses of 3₂-helices, a-helices and of the change in residual
entropy of a protein upon chemical modification. Part of the long-term goal of
understanding the formation, function and stability of proteins requires that all
conformations be accurately assigned and structurally understood. By a statistical
analysis of 258...
Protein kinases are an abundant class of enzymes which play important roles in numerous signal transduction systems. Arabidopsis TOUSLED kinase is a serine/threonine kinase which is essential for cell-cell communication within the shoot meristem. TOUSLED is encoded by a single gene in Arabidopsis. Recessive mutants in this gene show mild...
Surimi is a seafood product which is used to manufacture restructured products
such as artificial crab and lobster. Surimi is produced from fish fillets by washing to
remove sarcoplasmic proteins and increase the concentration of myofibrillar proteins, and
mixing with cryoprotectants. A valuable attribute of surimi is its ability to...