Storm events can pulse nutrients and carbon from soils and provide an important subsidy to food webs in oligotrophic streams and lakes. Bacterial nutrient limitation and the potential response of stream aquatic bacteria to storm events was investigated in arctic tundra environments by manipulating both water temperature and inorganic nutrient...
Dramatic increases in research in the area of microbial biofuel production coupled with
high-throughput data generation on bioenergy-related microbes has led to a deluge of
information in the scientific literature and in databases. Consolidating this information and
making it easily accessible requires a unified vocabulary. The Gene Ontology (GO) fulfills...
During traditional boat-based surveys of marine megafauna, behavioral observations are typically limited to records of animal surfacings obtained from a horizontal perspective. Achieving an aerial perspective has been restricted to brief helicopter or airplane based observations that are costly, noisy, and risky. The emergence of commercial small unmanned aerial systems...
Whitebark pine (Pinus albicaulis) inhabits an expansive range in western North America, and it is a keystone species of subalpine environments. Whitebark is susceptible to multiple threats – climate change, white pine blister rust, mountain pine beetle, and fire exclusion – and it is suffering significant mortality range-wide, prompting the...
The dataset here is used as a document file for presenting supplementary videos in the dissertation Tao, K. (2018). Investigating protein-lipid-membrane interactions in Plant Cells using Bimolecular Fluorescence Complementation (Doctoral dissertation).
Keywords: Protein, Lipid, Membrane, Plant Cells, Fluorescent Imaging
This dataset is a compilation of all published denudation rates derived from in-situ cosmogenic radionuclides as of 2016. All sites have been geo-referenced (WGS 84). Geo-referenced locations are then used to extract the yearly aridity index for each site from the CGIAR global Aridity dataset (https://cgiarcsi.community/data/global-aridity-and-pet-database/). Denudation rates are used...
The accessions used to create the Snap Bean Diversity Panel (SnAP) were 378 snap bean cultivars and germplasm lines selected from North American and European germplasm. This panel supplements the 150 member Bean CAP snap bean diversity panel (SBDP) with an additional 228 snap bean accessions. The Bean CAP SBDP...
The accessions used to create the Snap Bean Diversity Panel were 150 snap bean (Phaseolus vulgaris) cultivars and breeding lines selected from North American and European germplasm to create a population that is representative of the snap bean market class from a historical and geographical viewpoint. This panel was developed...
Grapevine leafroll disease (GLD) is one of the most important grapevine viral diseases affecting grapevines worldwide. The impact on vine health, crop yield, and quality is difficult to assess due to a high number of variables, but significant economic losses are consistently reported over the lifespan of a vineyard if...
Bud dormancy in grapevine is an adaptive strategy for the survival of drought, high and low temperatures and freeze dehydration stress that limit the range of cultivar adaptation. Therefore, development of a comprehensive understanding of the biological mechanisms involved in bud dormancy is needed to promote advances in selection and...
Mycobacterium abscessus (MAB) is an opportunistic pathogen responsible for infections in immunocompromised or immunosuppressed patients. Even with long-term treatment with multiple antibiotics, MAB pulmonary infections have high rates of treatment failure. Within the host, MAB is phagocytosed by key innate immune cells macrophages. The TLR2 and TLR4 are two critical...
In the study of any developmental process, whether in plants, animals, or other models, it is prudent to consider the developmental position of the subjects being measured. This is generally not taken into consideration in grapevine studies despite a growing body of literature documenting that fruits within clusters develop and...
Zoxamide is the sole β-tubulin inhibitor registered for the control of oomycete pathogens. The current study investigated the activity of zoxamide against Phytophthora sojae and baseline sensitivity was established with a mean EC₅₀ of 0.048 μg/ml. The data is critical for monitoring changes in zoxamide-sensitivity in the field. Three stable...
Full Text:
: 20 May 2016
doi: 10.3389/fmicb.2016.00762
Edited by:
Vijai Kumar Gupta,
National University of
Proteinaceous compounds are abundant forms of organic nitrogen in soil and aquatic ecosystems, and the rate of protein depolymerization, which is accomplished by a diverse range of microbial secreted peptidases, often limits nitrogen turnover in the environment. To determine if the distribution of secreted peptidases reflects the ecological and evolutionary...
Root-knot nematodes (Meloidogyne spp.) are one of the most important global plant pathogens. Recent innovations in molecular techniques have allowed increased study of this important pest, including the ability to investigate associated microbiomes. This study used 16S rDNA metagenomic sequencing to characterize the microbiome of Meloidogyne hapla, the northern root-knot...
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microbiome: looking back and future perspectives. Frontiers in Microbiology, 5. https://doi.org/10.3389/fmicb
Modulation of gene expression through RNA interference is well conserved in eukaryotes and is involved in many cellular processes. In the oomycete Phytophthora, research on the small RNA machinery and function has started to reveal potential roles in the pathogen, but much is still unknown. We examined Argonaute (AGO) homologs...
Important progress in understanding replication, interactions with host plants, and evolution of closteroviruses enabled engineering of several vectors for gene expression and virus-induced gene silencing. Due to the broad host range of closteroviruses, these vectors expanded vector applicability to include important woody plants such as citrus and grapevine. Furthermore, large...
Managers of forest and plantation ecosystems are encountering growing problems involving plant pathogens and the expanding geographic ranges of these pathogens. Historically unexposed stands are exceptionally vulnerable when a non-native pathogen is introduced because these pathogens can cause devastating disease in a host population that lacks co-evolved resistance mechanisms. One...
Neisseria gonorrhoeae is an exquisitely adapted, strictly human pathogen and the causative agent of the sexually transmitted infection gonorrhea. This ancient human disease remains a serious problem, occurring at high incidence globally and having a major impact on reproductive and neonatal health. N. gonorrhoeae is rapidly evolving into a superbug...
The lagoons spanning Alaska’s Beaufort Sea coast provide a unique habitat for arctic wildlife. These lagoons and the food webs they support face extreme seasonality with nine months of ice cover followed by a spring thaw that pulses a large amount of freshwater and nutrients into the lagoons. Bacteria link...
Iron (Fe) and copper (Cu) are essential cofactors for microbial metalloenzymes, but little is known about the metalloenyzme inventory of anaerobic marine microbial communities despite their importance to the nitrogen cycle. We compared dissolved O₂ , NO₃⁻, NO₂⁻, Fe and Cu concentrations with nucleic acid sequences encoding Fe and Cu-binding...
Complex symbioses between animal or plant hosts and their associated microbiotas can involve thousands of species and millions of genes. Because of the number of interacting partners, it is often impractical to study all organisms or genes in these host-microbe symbioses individually. Yet new phylogenetic predictive methods can use the...
While there has been growing interest in the gut microbiome in recent years, it remains unclear whether closely related species and strains have similar or distinct functional roles and if organisms capable of both aerobic and anaerobic growth do so simultaneously. To investigate these questions, we implemented a high-throughput mass...
The vast marine deep biosphere consists of microbial habitats within sediment, pore waters, upper basaltic crust and the fluids that circulate throughout it. A wide range of temperature, pressure, pH, and electron donor and acceptor conditions exists—all of which can combine to affect carbon and nutrient cycling and result in...
To understand mechanisms linking ecosystem processes and microbial diversity in freshwater ecosystems, bacterial productivity and the metacommunity dynamics of species sorting and mass effects were investigated in an 18 ha headwater lake in northern Alaska. On most sampling dates, the phylogenetic composition of bacterial communities in inflowing streams (inlets) was...
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biogeography. Frontiers in Microbiology, 5:82.
doi:10.3389/fmicb.2014.00082
10.3389/fmicb.2014.00082
Previous studies of coral viruses have employed either microscopy or metagenomics, but few have attempted to comprehensively link the presence of a virus-like particle (VLP) to a genomic sequence. We conducted transmission electron microscopy imaging and virome analysis in tandem to characterize the most conspicuous viral types found within the...
Microbial communities are responsible for the bulk of biogeochemical processing in temporary headwater streams, yet there is still relatively little known about how community structure and function respond to periodic drying. Moreover, the ability to sample temporary habitats can be a logistical challenge due to the limited capability to measure...
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headwater streams: a
cross-system comparison. Frontiers in Microbiology, 6, 522.
doi:10.3389/fmicb
Stony corals are the ecosystem engineers of the vital, dynamic, and complex marine ecosystem known as coral reefs. Globally, coral reefs are undergoing degradation from multiple anthropogenic stressors. Coral reef organism holobionts, or the host along with its microbial components, are key to reef ecosystem success and functioning. Marine microbes...
To determine if a storm event (i.e., high winds, large volumes of precipitation) could
alter concentrations of Vibrio vulnificus and V. parahaemolyticus in aquacultured oysters
(Crassostrea virginica) and associated surface water and sediment, this study followed a
sampling timeline before and after Hurricane Irene impacted the Chesapeake Bay estuary in...
Kombucha is a fermented sweet tea beverage of Asian origin which has become popular in the West due to its distinct flavor and possible health benefits. Kombucha is fermented by inoculating sweet tea with yeast and bacteria until a pellicle, referred to as a SCOBY (symbiotic culture of bacteria and...
Grapevine Trunk Diseases (GTDs) are caused by a group of fungal pathogens that attack the xylem tissue of mature grapevines worldwide. In the past 30 years, their incidence has increased, both in emerging grape-producing regions, as well as those with an extended history of viticulture. In the same time span,...
Marine sponges are vital components of benthic and coral reef ecosystems, providing
shelter and nutrition for many organisms. In addition, sponges act as an essential carbon
and nutrient link between the pelagic and benthic environment by filtering large quantities
of seawater. Many sponge species harbor a diverse microbial community (including...
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Microbiology, 5, 581.
doi:10.3389/fmicb.2014.00581
10.3389/fmicb.2014.00581
Frontiers Research Foundation
Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of Johne’s disease, a chronic granulomatous enteritis that plagues domestic and wild ruminants globally. During the silent stages of Johne’s disease, infected animals intermittently shed bacteria for years prior to clinical diagnosis during advanced disease stages. This strategy allows MAP to...
Ubiquitous pathogen Pseudomonas aeruginosa coordinates population-wide activation of virulence genes through quorum sensing (QS), where individuals transition from solo to social behaviors to benefit the population. QS is complex and mechanisms vary from species to species, but the concept is common to the bacterial world and has implications in various...
Volatile organic compounds (VOCs) play an integral role in climate change and as a carbon source for organisms living in a marine environment. These compounds have many sources, but the impacts of biogenically produced VOCs is not fully known. Phaeodactylum tricornutum was studied under axenic ideal growth conditions to determine...
Grapevine red blotch virus (GRBV), the causal agent of red blotch disease (RBD) in grapevine, Vitis vinifera L., is an emerging pathogen of significance to the wine grape industry of Oregon, USA. To address knowledge gaps of GRBV epidemiology, spread of GRBV was evaluated in Oregon vineyards over four years....
Methane (CH₄) is a valuable fuel, constituting 70–95% of natural gas, and a potent
greenhouse gas. Release of CH₄ into the atmosphere contributes to climate change.
Biological CH₄ production or methanogenesis is mostly performed by methanogens, a
group of strictly anaerobic archaea. The direct substrates for methanogenesis are H₂
plus...
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Ontology. Frontiers in Microbiology, 5, 634.
doi:10.3389/fmicb.2014.00634
10.3389/fmicb.2014.00634
Agricultural and companion animals are integral to our way of life as they provide us with nourishment, financial resources, transportation, and companionship. However, pets, managed animals in the agricultural industry, as well as wild populations of food animals are all subject to health and mortality risks. The gut microbiome is...
Seagrasses and coral reefs play important roles in nutrient cycling, coastal protection, and maintaining marine biodiversity. However, these coastal marine organisms are declining globally due to anthropogenic stressors, such as rising ocean temperatures, ocean acidification, and eutrophication. These organisms live in close association with their microbiomes, which can be beneficial...
Mycobacterium avium subsp. hominissuis (MAH) is an environmental bacteria that infects immunocompromised humans. MAH cases are increasing in incidence, making it crucial to gain knowledge of the pathogenic mechanisms associated with the bacterium. MAH infects macrophages and after several days the infection triggers the phagocyte apoptosis. Many of the intracellular...
Agricultural land management, such as fertilization, liming, and tillage affects soil properties, including pH, organic matter content, nitrification rates, and the microbial community. Three different study sites were used to identify microorganisms that correlate with agricultural land use and to determine which factors regulate the relative abundance of the microbial...
Copper and iron are essential micronutrients that are required by marine primary producers for a variety of metabolic processes. Over 99% of both copper and iron are bound within organic complexes in the marine environment, however the molecular identity of many of these complexes remains unknown. The speciation of these...
Septoria canker remains the most important disease of poplars in intensively managed forest plantations. Genetic resistance has long been considered the best way to manage for this disease. Transgenic resistance mediated by RNA silencing against pathogens and pests (HIGS: host-induced gene silencing) has shown promise in other pathosystems but has...
Bacteria are abundant in marine environments. They play important roles in nutrient cycling and form symbiotic interactions with eukaryotes. However, the vast majority of bacterial taxa are difficult to maintain in laboratory cultures, meaning that most microbiological research of the past century has focused on a small subset of bacteria....
The wellbeing of the Willamette basin in Oregon depends on the river ecosystem’s microbial communities, which control primary production and the biological processing of nutrients, pollutants, and organic matter. Yet, only a few studies have investigated the diversity of the microbial communities of the Willamette basin. This paper examines how...
Oregon strawberries are known for their high quality, intense flavor, sweetness, and rich red color. With optimal weather conditions, including rainy and mild seasons, Oregon has one of the best strawberry farming environments. Amongst the factors for a suitable strawberry farming environment, soil significantly influences strawberry growth and quality. The...
Freshwater systems cycle carbon along a spatial and temporal biogeochemical continuum, across which ecosystem processes contribute to transformations of organic matter (OM). Various ecological constraints impact rates OM transformation and production and consumption of the energetic end of respiration, methane. Microbiological processing and complete reduction of carbon substrates to methane...
Listeria monocytogenes is the third most deadly foodborne pathogen in the United States. The young and elderly, as well as pregnant and immunocompromised people are the population most susceptible to serious illness and death from listeriosis infections.
Unlike most foodborne pathogens, L. monocytogenes does not live a solely enteric lifestyle....
The effects of fire on above-ground forest systems have been extensively studied. However, research on fire effects on belowground processes is lacking. While the soil microbiome is understood to be vital to conifer growth and regeneration, the complexity of soils means that research on the topic has largely been accomplished...
Biotic and abiotic processes at continent-ocean interfaces cycle a disproportionate mass of carbon and nutrients relative to their global surface area, and microbial activity is a principal determinant of organic and inorganic matter flux in these transition zones. Most studies using modern high-throughput ‘omics techniques to link microorganisms with costal...