This study was an ethnographic case study of a
Vietnamese unaccompanied minor in a post secondary setting.
There were two guiding questions for the study: (1) How
does the subject perceive language as it relates to
educational experience? (2) What kinds of observable
personality, cognitive, or affective factors have
contributed...
Vaccinia virus infected monkey kidney cells had been previously shown
to have an increased ribonucleoside diphosphate reductase (RR) activity. DNA
from mutant virus resistant to hydroxyurea were digested with restriction
endonucleases and were shown to have substoichiometric amounts of the Hind
III F fragment. Additional information from Southern blotting experiments...
The Thomas Kay Woolen Mill operated between 1890 and 1962. The mill produced predominately woolen blankets and woolen fabrics for outerwear garments. Mission Mill Museum Association purchased the woolen mill after its closure in order to develop the property as a museum. The purpose of this study was to provide...
The Thomas Kay Woolen Mill once manufactured cloth with a
reputation renowned throughout the United States. This study is an
attempt to report the history of the mill. Data are categorized by:
the Kay family; production including plant, machinery, raw materials
used, goods manufactured; management including the effects of
national...
The emerging field of regenerative medicine is mainly approached by two different aspects. First is the use of stem cell based models to generate a suite of differentiated cells for therapeutic applications and the alternative approach is to utilize the non-mammalian models that have the inherent capacity to regenerate their...
The intent of this work is to extend Implicit Monte Carlo Diffusion (IMD)[Gen.
2001] to account for frequency dependence and to incorporate the difference formulation[Szo. 2005] as a source manipulation variance reduction technique. This
work shows the derivation of the probabilities and the associated proofs which
govern the frequency dependent...
Shallow landslides are destructive natural hazards that result in widespread losses, both of infrastructure and lives, throughout the world. To help mitigate the impact of landslide hazards, they are commonly characterized across a regional scale by (1) their location and likelihood using susceptibility maps, (2) their magnitude and frequency using...
Markov chains have long been used to sample from probability distributions and simulate dynamical systems. In both cases we would like to know how long it takes for the chain's distribution to converge to within varepsilon of the stationary distribution in total variation distance; the answer to this is, called...
Lucina floridana is an intertidal clam that contains intracellular, sulfur-oxidizing
symbiotic bacteria in its gills. These bacteria are chemolithoautotrophic, using the energy
from the oxidation of reduced sulfur compounds to power carbon fixation. Although
sulfide is an energy-rich compound, it is also toxic to aerobic metabolism and autooxidizes
in the...
The daily urinary excretion of taurine was studied in six women,
22 to 36 years old. Five subjects collected 24-hour urine specimens
for 10 days, and another for 37 days. The subjects were free from
any known metabolic disorder. They consumed their normal diet.
Urinary taurine was determined by the...
Old women face a unique set of obstacles in their
quest for empowerment. In this study the concept of
empowerment is explored by politicizing issues of personal
safety. The most significant factors that impede an old
woman's sense of safety and control include oppression,
primarily ageism and sexism, along with...
A rapid virus detection method was developed for Infectious Hemotopoietic Necrosis Virus (IHNV). The virus detection method could be applied to Infectious Pancreatic Necrosis Virus (IPNV) under certain conditions. It was possible to detect IHNV to a lower limit of 1.7 X 10⁴ Tissue Culture Infectious Dose for 50% (TCID₅₀)...
This project examines the landscape of the farming community of Beagle, Oregon
prior to and during the Second World War and the effect on it due to
the construction of Camp White, a World War II training facility. The Beagle
landscape is examined through the prism of current theory that...
A sample of 893 Oregon individuals from a Western Regional
Agricultural Experiment Station Project (W-159 "Consequences of Energy
Conservation Policies for Western Region Households") was used to
analyze consumer attitudes toward potentially restrictive energy
conservation regulations. Belief in the seriousness of the energy
problem, a psychological measure of internal control...
Biological organisms, phenomena and strategies, herein referred to as biological systems, provide a rich set of analogies that can be used to inspire engineering innovation. Biologically-inspired, or biomimetic, designs are publicly viewed as creative and novel solutions to human problems. Moreover, some biomimetic designs have become so commonplace that it...
Ribonucleoside diphosphate reductase (RR) from vaccinia virus was recently cloned and overexpressed rn Escherichia coli. The amino acid sequence identities of the small and large subunits between the mouse and the vaccinia virus reductase are approximately 80 and 72 percent, respectively. In addition, vaccinia virus RR displays similar complex allosteric...
The dihydrofolate reductase gene of bacteriophate T4
has been investigated at the structural level to elucide the
unusual regulation of expression of this gene and possible
evolutionary relationships between the ohage and drug resistance
factors-encoded dihydrofolate reductases (DHFR).
A 1.1-kilobase-pair restriction fragment of the T4
multiple mutant dec8 containing frd,...
Ribonucleotide reductase is a remarkable enzyme that catalyzes the rate-limiting
step in the synthesis of the 2'-deoxynucleoside triphosphates. The intent of this project
was to characterize the ribonucleotide reductase encoded by the orthopoxvirus,
vaccinia. The first objective was to study the structural and functional features of the
viral small subunit...
Motivation: Many robots such as legged robots and prosthetic hands/arms are designed to interact with uncertain and time-varying environments to accomplish their tasks.
Observations on humans and animals during their daily tasks suggest that they adapt their leg compliance while traversing with different velocities on different grounds and adjust their...
In order to maintain genetic stability in eukaryotes, tight regulation of the relative sizes of deoxyribonucleoside triphosphate (dNTP) levels inside the cell is essential for optimal fidelity of DNA replication. Ribonucleotide reductase (RNR) is the enzyme responsible for proportional production of DNA precursors. Studies on regulation of this enzyme, the...
It is well known that the mitochondrial genome has a much higher spontaneous mutation rate than the nuclear genome. mtDNA mutations have been identified in association with many diseases and aging. mtDNA replication continues throughout the cell cycle, even in post-mitotic cells. Therefore, a constant supply of nucleotides is required...
The first committed step in DNA biosynthesis occurs by direct reduction of
ribonucleotides. This reduction is catalyzed by ribonucleotide reductase (RNR), an
enzyme which uses a unique radical mechanism to facilitate the transformation. All four
DNA precursors are synthesized by a single enzyme. Therefore, an intricate pattern of
regulation has...
Imbalanced deoxyribonucleoside triphosphate (dNTP) pools are mutagenic for DNA synthesis in both intact cells and cell-free replication systems. Almost certainly, such mutagenesis involves competition between correctly and incorrectly base-paired precursors at replication sites. However, there are certain differences between the intact cell and cell-free systems that do not always allow...
Movement intent decoders, which interpret volitional movement intent from human bioelectric signals, can be incorporated into modern neuroprostheses to offer people living with limb loss or paralysis the potential to regain their lost motor control. Machine learning methods have become the research standard for continuous decoders with high degrees of...
This dissertation describes an investigation of DNA precursor supply for mitochondrial and nuclear DNA synthesis in HeLa cells and Mouse L-cells. HeLa cells were used for the quantitation of cellular and mitochondrial deoxyribonucleoside triphosphate (dNTP) and ribonucleoside triphosphate (rNTP) pools and of changes in pools in response to treatment with...
Significance: Movement intent decoding algorithms can interpret human bioelectrical signals to control prosthetic limbs with many degrees of freedom (DOFs). This work involves decoding volitional movement intent from surface electromyogram (sEMG) signals to control prosthetic arms. To train these algorithms, patients flex their muscles to “follow” a movement prompt, and...
A crucial factor in determining the accuracy of DNA replication is
maintenance of a balanced supply of deoxyribonucleoside triphosphates
(dNTPs) at replication forks. Perturbation of dNTP biosynthesis can
induce dNTP pool imbalance with deleterious genetic consequences,
including increased mutagenesis, recombination, chromosomal
abnormalities and cell death. Using the T4 bacteriophage system,...
The effects of oligoribonucleotides of identical or similar sequences to a
ribosomal protein binding site, specifically S8, on prokaryotic 30S ribosomal assembly
were investigated in Escherichia coli. The oligoribonucleotides were expected to bind to
the target protein S8 and block its incorporation into the 30S subunit, resulting in a nonfunctional...
Vaccinia virus-infected animal cells have been used to
study the interactions between the replication of
deoxyribonucleic acid (DNA) and the biosynthesis of its
nucleotide precursors. Some antimetabolites that inhibit
DNA replication have as their targets enzymes of nucleotide
biosynthesis. Furthermore, the disruption of nucleotide
metabolism can alter the fidelity of...
Deoxyribonucleoside triphosphate (dNTP) concentrations measured in cells are not symmetric. dGTP almost always represents only 5-10% of the total dNTP pools in cells. In an in vitro replication system involving semiconservative replication from an SV 40 origin, the mutation frequency of an M13 phagemid replicated by human cell extracts in...
The ability to faithfully replicate DNA is dependent upon the maintenance
and regulation of its precursors, the deoxyribonucleoside triphosphates.
Enzymes encoded by the bacteriophage T4 have been widely used as models
of biochemical processes. A body of evidence supports the concept that the
bacteriophage T4 enzymes involved in deoxyribonucleotide biosynthesis...
Although the Escherichia coli host has almost all of the enzymes necessary to
synthesize nucleotides needed for bacteriophage T4 DNA replication, phage genes
expressed early in infection encode enzymes for de novo DNA precursor biosynthesis
and salvage from degraded host DNA. Eight early enzymes and two host enzymes
comprise the...
There are nearly two million limb amputees living in the United States of America. Loss of limbs results in profound changes in one's life. However, the underlying neural circuitry and much of the ability to sense and control movements of their missing limb is retained even after limb loss. This...
The seeds of Douglas fir, a representative of the gymnosperm
flora, were studied at six stages of germination to discern the structures
of food reserves and to study ultrastructural changes occurring
during germination in the embryo and resulting seedlings.
The materials were fixed in three fixatives, dehydrated with
ethanol series,...
A set of genes which show developmental and tissue
specificity are expressed late in embryo maturation during
grain formation in wheat (Triticum aestivum). Two members
of this maturation gene set (MGS) are Em and triticin, a 7S
globulin. Results presented here show that during
embryogenesis, the expression of Em mRNA...
Red fir (Abies magnifica) is a high elevation conifer generally growing between an altitude of 1,400 and 2,700 meters. In California, red fir grows in the Sierra Nevada, the Klamath Mountains, the eastern edges of the northern Californian Coast Ranges, and in the southern Cascades. Red fir commonly grows in...
The quality of macromolecular crystal structures depends, in part, on the quality and quantity of the data used to produce them. Here, we review recent shifts in our understanding of how to use data quality indicators to select a high resolution cutoff that leads to the best model, and of...
A study of malolactic bacteria of the Leuconostoc
genus, especially L. oenos, and their bacteriophages was
conducted. A search for the presence of infectious
bacteriophages in grapes, musts, and juices employed in
malo-lactic fermentation (MLF) was conducted; no
evidence, either physical or biological, was found for
the existence of these...
Genomic instability drives tumorigenesis, but how it is initiated in sporadic neoplasias is unknown. In early preneoplasias, alterations at chromosome fragile sites arise due to DNA replication stress. A frequent, perhaps earliest, genetic alteration in preneoplasias is deletion within the fragile FRA3B/FHIT locus, leading to loss of Fhit protein expression....
These studies were conducted to examine the
influence of several variables on the growth hormone binding
protein (GHBP) in serum of pigs. Continuous long term
porcine somatotropin (pST) injections (daily for 6-7 wk)
increased GHBP activity (p < .05). However, periodic short
term pST injections (daily, every second d or...
Growth hormone binding protein (GHBP) may be an important
factor in the regulation of growth as well as an indirect,
less invasive way of predicting the status of growth hormone
receptors. Several factors (age, nutritional status, sex,
and glucocorticoid administration) have been reported to
influence circulating growth hormone (GH) levels,...
This study was conducted to determine the effects of
shipping stress on the immune system in domestic lambs (Ovis
aries) and to determine the potential of the thymic peptide,
thymulin, to reduce those effects of stress on the immune
system. Treatments consisted of no shipping (as unstressed
controls), shipping (as...
The continuation of geoarchaeological investigations at Tseriadun (35CU7) contributes to the understanding of the site's depositional history and the associated environments, thereby identifying the long term geomorphic change in the area and providing the environmental context for local human activity as it is reflected in the archaeological record. Meeting this...