Hop, Humulus lupulus L. is a dioecious perennial species. The female hop plant produces flowers that mature into ‘cones’ which are used as a raw material in the beer brewing process to impart bitterness, flavor, and aroma. The craft beer industry in the current global beer market continues to experience...
Genetic and morphological characteristics of an architectural mutant in common beans were studied. The mutant had shiny, dark green leaves, overlapping leaflets, short petioles and a reproductive terminal bud even though the line did not carry the fin gene. Branching was nearly absent resulting in a single stem vine. This...
An apparatus and method for calibrating planar laser induced fluorescence using a two-dye, two-color method was developed. A laser sheet was expanded from a 473 nm laser to excite dye solutions into higher energy states then fluoresced through spontaneous emission. The two fluorescent dyes selected for research and development were...
The severity of wildfires around the globe is increasing. At the same time, urban development is expanding outward into areas where severe fires occur. There is an increased risk of home loss to fires in areas where severe fires and urban expansion meet. Ignition of homes or nearby fuel is...
Thymidine kinase is a key enzyme in the nucleotide salvage pathway,
catalyzing the production of dTMP from thymidine and ATP. In order to
identify the structural features of the TK protein and/or primary amino
acid sequences which contribute to the catalytic and regulatory activities of
this enzyme, an in vitro...
Background: Body Mass Index (BMI) is used as a quick and inexpensive tool to measure populations at risk for a number of metabolic and chronic conditions. In part due to its simplicity, BMI has been criticized for its inaccurate prediction concerning health outcomes. Due to the diversity in the United...
β-catenin is a multi functional protein that is involved in cell-cell adhesion and cell signaling. In non-stimulated cells, β-catenin is tightly down-regulated by GSK-3β-dependent phosphorylation at Ser and Thr residues, followed by rapid ubiquitination and proteasomal degradation. It is well established that mutations within the regulatory GSK-3β region lead to...
The persistent color (pc) trait in snap bean (Phaseolus vulgaris L.) belongs to a member of the cosmetic stay-green gene family. It is considered very desirable by snap bean breeders for its impacts on pod quality. Persistent color imparts a uniform dark-green color to pods and expresses in seeds as...
Health care providers, including complementary and alternative medical (CAM) practitioners, exert a significant influence on parental pediatric vaccination decisions. Use of CAM therapies is increasing in Oregon. Concomitantly, there has been a decade-long increase in parental vaccine refusal in Oregon, rising from 1 to 5 percent from 2000-2009. For example,...
Advances in technology such as 3D digital scanning and spatial analysis software have provided archaeologists with novel data. Specifically, these methods increase the researcher's ability to measure artifact morphology and past networks of cultural transmission, to potentially track the movement of past peoples and ideas through space and time. This...
Interspecific hybridizations between P. vulgaris and P. acutifolius
and between P. vulgaris and P. lunatus were investigated. Prefertilization
events were similar in inter- and intra-specific crosses. Fertilization
was completed in all crosses and the time of fertilization was
dependent on the maternal parent, reflecting differences in the time of
maturation...
Mutation of repetitive DNA by repeat-induced point mutation (RIP) is a process that occurs in many filamentous fungi of the Ascomycota during the sexual cycle. Concurrently, direct DNA repeats are often deleted by homologous recombination at high frequency during the sexual cycle. Thus, the processes of RIP and deletion compete...
The effects of modifying air temperature, soil temperature,
and soil moisture levels on flowering, fruiting, and chemical composition
of Tendercrop snap beans were studied in experiments in
the field and in the greenhouse during 1961, 1962, and 1963.
High maximum temperatures of 95-105° F. during bloom reduced
the percent set...
Mutation analysis is the gold standard for evaluating test-suite adequacy. It involves exhaustive seeding of all small faults in a program and evaluating the effectiveness of test suites in detecting these faults. Mutation analysis subsumes numerous structural coverage criteria, approximates fault detection capability of test suites, and the faults produced...
Declines in Greater Sage-Grouse (Centrocercus urophasianus, hereafter sage-grouse) populations could be attributed to low chick survival, which may be influenced by the availability of food and cover at sites used by females rearing broods. Habitat attributes important to broods may vary regionally; thus, it is necessary to understand factors affecting...
Maintaining genome integrity is essential for an organism, as mutation
accumulation can lead to cancer, reduced fitness, and heritable diseases in offspring.
Therefore the study of mutations, how they are induced, and how they are prevented is
vital. Biomonitor systems are useful for understanding the relevant biological effects
of a...
Noise induced hearing loss is a subject of growing
concern. Over the past 15 years, several well-documented
studies have been completed which express the relationships
between industrial noise exposure and damage to normal and
pathological ears. This research has provided knowledge of
considerable importance to developing successful hearing
conservation programs....
Mutation analysis is a well-known method for measuring the quality of test suites. However, it is computationally intensive compared to other measures, which makes it hard to use in practice. Choosing a smaller subset of mutations to run is a simple approach that can alleviate this problem. Mutation operator selection...
Mutation analysis is often used to compare the effectiveness of different test suites or testing techniques. One of the main assumptions underlying this technique is the Competent Programmer Hypothesis, which proposes that programs are very close to a correct version, or that the difference between current and correct code for...
The enzymes of dNTP synthesis in T4 infection associate to form a multienzyme complex, the T4 dNTP synthetase complex, facilitating the flow of metabolites en route to dNTPs, and their subsequent flow into DNA. Study of protein-protein interactions helps one to understand how the enzymes in the complex are organized...
This dissertation is an attempt at developing a method for the analysis and estimation of the effects of disruptions due to uncertainties. Such uncertainties may result from design changes and engineering drawing delays in large-scale, complex, research and development, or construction projects.
In order to provide management with a simple...
The accessions used to create the Snap Bean Diversity Panel (SnAP) were 378 snap bean cultivars and germplasm lines selected from North American and European germplasm. This panel supplements the 150 member Bean CAP snap bean diversity panel (SBDP) with an additional 228 snap bean accessions. The Bean CAP SBDP...
A time-dependent, spectral, barotropic model and a similar
two-layer primitive equation model are developed to investigate the
planetary-scale wave responses to various types of large-scale
forcing: vorticity and heat sources, and sea surface temperature (SST)
anomalies. Both models are linearized about the zonal mean states of
January climatology.
The characteristics...
Hepatocellular carcinoma (HCC) is currently one of the top lethal cancers with an increasing trend. Deregulation of MYC in HCC is frequently detected and always correlated with poor prognosis. As the zebrafish genome contains two differentially expressed zebrafish myc orthologs, myca and mycb, it remains unclear about the oncogenicity of...
DNA mismatch repair (MMR) is an evolutionary conserved process that functions to maintain genomic integrity through the correction of mismatches that have escaped proofreading. Mutations in the MMR gene Mlh1 are associated with approximately 50% of all cases of Lynch syndrome, a hereditary predisposition to colorectal cancer, through varying and...
Inheritance of the volatile components in bush snap beans,
O.S.U. 58-110 X 'Romano' cross and reciprocal, was determined
using gas-liquid chromatographic technique with gas-entrainment
on-column trapping. A sample of 10 to 14 pods averaging 5 grams
from a single plant was found to be adequate for F₁, F₂, and backcross...
Rice is an important crop that feeds almost half the world population. As climate change models predict floods, drought and extreme temperatures in rice production areas, the need to better understand the genetic basis of adaptation and tolerance to abiotic and biotic stresses is vital to sustain our food supply....
Published April 1975. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog
Published June 1960. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog
Saccharomyces cerevisiae contains three NAD⁺/NADH kinases, one of which is localized in
mitochondria and phosphorylates NADH in preference to NAD⁺. Strand et al (Euk. Cell
2:809 (2003)) reported that a yeast mutation in POS5, which encodes the mitochondrial
NADH kinase, is a mutator, specific for mitochondrial genes. Because of the...
Genomic instability underlies diseases of unregulated cell growth that result in
cancers and developmental abnormalities in humans. Similar genome destabilizing
mechanisms are used to create genetic variety in crops for use in breeding and trait
development. Errors that occur during DNA replication may cause mutations if
they are not corrected...
The in planta growth of three wild type strains of phytopathogenic bacteria and three nonpathogenic mutant strains was studied to determine if mutations eliminating the ability of the mutant strains to cause disease had affected their growth in leaf tissue. The mutant strains were obtained by Tn5 mutagenesis of Pseudomonas...
The easy picking characteristic, expressed as low pod detachment force (PDF),
offers a way to decrease pod damage in bush green beans mechanically harvested for
processing and fresh market, and makes hand picking easier and faster. This study was
undertaken to examine inheritance in a cross of two green bean...
Though mutation analysis is the primary means of evaluating the quality of test suites, though it suffers from inadequate standardization. Mutation analysis tools vary based on language, when mutants are generated (phase of compilation), and target audience. Mutation tools rarely implement the complete set of operators proposed in the literature,...
For ras gene mutation analysis in the rainbow trout (Oncorhynchus
mykiss) model system, a partial trout ras sequence was identified
using the polymerase chain reaction (PCR). Two synthetic
oligonucleotides based on rat K-ras gene sequence were used as
primers for the PCR procedure. A 90 base pair (bp) sequence,
referred...
An important impact of the genome technology revolution will be the elucidation of mechanisms of cancer pathogenesis, leading to improvements in the diagnosis of cancer and the selection of cancer treatment. Integrated with current well-studied massive knowledge and findings about the role of protein-coding mutations in cancer, demystifying the functional...
An impediment to use of exotic and bioengineered trees in many places is their propensity for spread by pollen and/or seeds. Our laboratory has been using gene editing to induce mutations in floral genes as means to impart stable and reliable genetic containment when this is desirable from social (markets,...
Snap beans are the vegetable form of dry bean (Phaseolus vulgaris L.) with low fiber, stringless and round, succulent pods that are eaten in the immature stage. Seed companies commit significant resources to maintaining purity and uniformity of snap bean cultivars. While some variability may be introduced through outcrossing (beans...
Integrase catalyzes insertion of a retroviral genome into the host chromosome. Following reverse transcription, integrase binds specifically to the ends of the duplex retroviral DNA, endonucleolytically cleaves two nucleotides from each 3'-end (the processing activity), and inserts these ends into the host DNA (the joining activity) in a concerted manner....
Mismatch repair (MMR) system performs mainly three roles to maintain
genomic stability, correct DNA biosynthetic errors, ensure the fidelity of
genetic recombination, and in mammalian cells participate in the cellular
response to some DNA damages. Deficiencies in mismatch repair increase
mutation rates and cancer risks. In eukaryotes, the MMR system...