The transcription process of infectious hematopoietic necrosis virus (IHNV), a rhabdovirus of salmonid fish, was studied both in vivo and in vitro. Polyadenylated RNA from IHNV infected-salmon cells was resolved electrophoretically into five bands, one of which was found to contain two mRNA species. The molecular weights and intracellular molar...
The two segments of double-stranded RNA (dsRNA) from
infectious pancreatic necrosis virus (IPNV) were cloned
into plasmid vector pUC8. Two distinct sets of overlapping
clones were identified by restriction enzyme and Southern
blot analysis. Each of these sets was shown, by Northern
blot analysis, to be exclusively related to either...
Nucleic acid extracted from dormant cane phloem of
grapevines affected by grapevine stem-pitting disease (SP)
formed a high molecular weight (MW) band on 1.0% and 0.8%
agarose gels. The band was unique to diseased vines. Other
bands appeared from samples of both diseased and virus-free
vines, but did not associate...
Some positive-strand RNA plant viruses possess a transfer RNA-like structure (TLS) at the 3'-terminus of their genomic RNAs. The closest mimicry to tRNA is exhibited by the valylatable TLSs from tymoviruses and furo-like viruses, which are able to interact with key cellular tRNA enzymes: [CTP, ATP]:tRNA nucleotidyltransferase (CCA NTase), valyl-tRNA...
Bovine viral diarrhea virus (BVDV) is an enveloped, single-stranded positive-sense RNA virus. The BVDV genome is about 12.5 kb long and consists of a single open reading frame (ORF) flanked by untranslated regions at the 5' and 3' ends (5'-UTR and 3'- UTR, respectively). To investigate the role of cis-sequences...
Beet yellows virus (BYV) is a filamentous, positive-strand RNA virus that belongs to the family Closteroviridae. BYV particles encapsidate a 15.5 kb RNA and posses complex polar architecture. A long virion body is formed by the major capsid protein(CP), whereas the minor capsid protein (CPm) assembles a short tail that...
Three closely related isolates belonging to the A₁ serotype of infectious pancreatic
necrosis virus (IPNV) were selected for comparison, to provide insight into the nature of
variation in the virulence of IPN viruses. Brook trout fry (Salvelinus fontinalis) were
experimentally infected with the three isolates by immersion. Cumulative mortalities over...
Turnip yellow mosaic virus is a monopartite, plus sense RNA virus
infecting the Cruciferae, and is a model system for the study of RNA virus
replication. A cDNA clone (pTYMC) representing an infectious RNA
genome of the European isolate of TYMV was constructed and used to assess
the importance of...
Coreplication experiments have revealed that replication of
turnip yellow mosaic virus (TYMV) RNA in turnip protoplasts is cis-preferential.
Genomes encoding mutant p141 or p66, proteins
essential for virus replication, were inefficiently rescued by a
helper genome. One model for the cis-preferential replication of
TYMV is that p66 and p141 form...
More than half of the recognized genera of positive strand RNA viruses employ polyprotein processing as one of the strategies for their genome expression. Normally, this processing is mediated by virus-encoded proteinases that belong to the trypsin-like or papain-like family. In particular, papain-like, leader proteinases were found in diverse families...
Humans and viral disease are inextricably intertwined. Viral disease plays an immeasurable role in human life, from the disease and economic burden associated with every facet of contending with human viral disease, to managing the consequences of viral disease in organisms important to our food supply, economy, and entertainment. The...
RNA isolated from BAI strain A (Myeloblastosis) Avian Tumor
Virus can be fractionated into two fractions on a sucrose density
gradient, one of higher molecular weight and one of low molecular
weight. The low molecular weight (LMW) virus RNA was found to
chromatograph the same as E. coli tRNA on...
The A segment of infectious pancreatic necrosis virus (IPNV)
is expressed as a polyprotein encoding three primary gene
products, VP2, NS and VP3, from a large open reading frame. The
nucleotide sequence for the A segment of the Sp isolate of IPNV
was determined. The NS protein is the putative...
In this thesis, I studied the translation of dengue virus RNA using a luciferase reporter gene system in Vero cells. The dengue reporter mRNA construct, which harbors 5´-terminal viral nts and 3´-terminal nts, could be translated efficiently compared to an alpha globin reporter construct. The 5´-cap structure and 3´-untranslated region...