Twenty-six Holstein and 18 Jersey multiparous cows in a randomized block design were assigned by calving date and breed to a non-anionic prepartum diet (CTRL) or a treatment diet containing a commercial anionic salt supplement (Animate®). Control and treatment diets were formulated to a dietary anion-cation difference (DCAD) of 21.3...
We hypothesized that calpain activity is elevated in response to muscle damage. To test this hypothesis, we examined the degradation of α-fodrin into its 150 and 145 kDa fragments following either 20 eccentric or isometric contractions. In addition, experiments were performed in the presence or absence of E-64-d, a calpain...
The goal of this research was to characterize thermal habitat requirements for juvenile redband steelhead trout (Oncorhynchus mykiss gairdneri) in the South Fork John Day River (SFJD), Oregon using physiological indicators of fish condition. Physiological indices of fish condition measured were whole body lipid content and heat shock proteins, specifically...
Most vertebrates exhibit seasonality in many life history traits. Such seasonal rhythms are temporally organized via the transduction of environmental cues (e.g., photoperiod, temperature) into appropriate endocrine signals. However, among ectothermic vertebrates that undergo continuous winter dormancy, temperature is the only environmental cue available for synchronizing seasonal rhythms. Most intriguing...
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4.2 Effect of capture stress on (A) corticosterone and (B) melatonin
concentrations of
An experiment was conducted with 27 multiparous Holstein and 27 multiparous Jersey cows to determine if nicotinic acid (NA) supplementation during the prepartum phase of the transition period decreases lipolysis and improves dry matter intake and to determine if Holstein and Jersey cows respond similarly to NA supplementation. Cows were...
Prostate cancer is the most commonly diagnosed cancer in men, but rates around the world vary greatly. Americans have the highest incidence rates in the world, 4 to 5 times those of Spain. The hypothesis of this thesis was that vitamin D metabolism is a factor in prostate cancer development...
Two strains of Escherichia coli were studied to determine the effect of constitutive expression of the SOS DNA damage repair response on the activity of ribonucleotide reductase. Activity of the reductase and cellular deoxynucleoside triphosphate (dNTP) pools were both determined before and after exposure of the cells to ultraviolet radiation....
Sperm mobility is defined as the net movement of a sperm population against resistance. Previous research demonstrated that the cell-permeant Ca2+ indicator rhod-2 AM gave a fluorescent signal predominantly in the head region of fowl sperm that differed between sperm mobility phenotypes. This thesis served to explore the role of...
This summary discusses the risk to human health associated with herbicide
application projects to control noxious weeds and poisonous plants on lands
administered by the USDA Forest Service in the Northern Region. The Northe!n
Region consists of approximately 211.8 million acres of National Forest System
(NFS) land in northern Idaho,...
Purpose:
The xanthophyll carotenoids lutein and zeaxanthin are concentrated in the macula lutea of the retina. It is thought that the two plant pigments may play a role in preventing the development of age-related macular degeneration (AMD). Exposure to damaging light plays a role in retinal deterioration and AMD. The...
Nares Strait is one of three main passages of the Canadian Archipelago that
channels freshwater from the Arctic Ocean to the North Atlantic. There are very few
observations regarding the role of this region on the present day Arctic freshwater budget,
and even less regarding the changes in freshwater fluxes...
Many members of the Phylum Cnidaria are mutualistic with unicellular dinoflagellates belonging to the genus Symbiodinium. Corals are the most widely recognized example of these associations due to their key ecological importance in coral reef ecosystems where they serve as the structural and trophic foundation of these rich ecosystems. Coral...
Two experiments were conducted looking at both acute and sub-acute laminitis. An acute nutritional induction model was used to examine whether blood profiles and gene expression are similar to other studies conducted using an endotoxin induction model. Twelve sheep were randomly assigned to three treatment groups which were control, 4-hour...
Overtly non-toxic doses of organochlorine (OC) insecticides perturb lipid homeostasis
in mice and rats. To increase understanding of how chlordecone (CD), an OC
insecticide, modulates cholesterol (CH) homeostasis, we determined the effect of CD
pretreatment on the tissue and hepatic subcellular distribution of exogenous [¹⁴C]CH. Corn oil alone or CD...
Representing the most common flavonoid consumed in the American diet, the flavanols and their polymeric condensation products, the proanthocyanidins, are regarded as functional ingredients in various beverages, whole and processed foods, herbal remedies and supplements. Their presence in food affects food quality parameters including astringency, bitterness, sourness, sweetness, salivary viscosity,...
In rats, sesame lignans increase plasma γ-tocopherol concentrations and inhibit γ-tocopherol degradation to its metabolite γ-carboxyethylhydroxychroman (γ-CEHC). To test if sesame lignan consumption inhibits γ- tocopherol metabolism in humans, muffins prepared with either corn oil (control) or sesame oil and an equimolar mixture of deuterium labeled d6-α- and d2-γ- tocopheryl...
Glutathione (GSH) is the predominant low molecular weight thiol antioxidant
in liver tissue. GSH plays an important role in maintaining the intracellular thiol redox
ratio as well as detoxification of electrophiles and xenobiotics. Aging leads to a significant decline (35%; P ≤ 0.05) in hepatocellular GSH levels. Using young (2-4...
Exercise increases B-vitamin (B6, B12, folate) dependent metabolic reactions, especially those related to energy production and the rebuilding and repairing of muscle tissue. These same B-vitamins are also important in maintaining low blood levels of homocysteine (Hcy), a cardiovascular disease (CVD) risk factor. Hcy levels rise when the metabolism of...
Polycyclic aromatic hydrocarbons (PAHs) are significant pollutants in aquatic environments. Many are carcinogenic and lead to DNA fragmentation and adduct formation in marine and freshwater organisms. Previous research demonstrates that rainbow trout (Oncorhynchus mykiss) compensate to long-term PAH dietary exposures and reduce the DNA damage.
The aim of this study...
Aerobic organisms have evolved many sensory mechanisms that allow
response to oxidants in the environment. One area of interest is the relationship
between the activity of the tumor suppressor protein p53 and the redox state of
thioredoxin. Human p53 activity is severely compromised in budding yeast lacking
thioredoxin reductase. Evidence...
There have been many studies that describe the protective degradation or metabolism of potentially harmful plant toxins, such as, mimosine from Leucaena leuconcephala, pyrrolizidine alkaloids from tansy ragwort (Senecio jacobaea), oxalate and some mycotoxins by rumen microbes. There are many cases of plant-related toxicoses suffered by ruminant animals where there...
The mechanisms by which the activities of phospholipase C-β (PLC-β) enzymes are negatively regulated have not been well defined. We used cardiac-derived ratmyoblast H9c2 cells to investigate possible modes of regulation of PLC-β3 enzymes,the only endogenous β isoform abundantly found in these cells. The PLC-β3population in these cells was detected...
The results of a review of six decades of existing literature for 50% lethal dose data across a range of phyla are presented. The collected 50% lethal dose data is limited to adult organisms subjected to acute doses of gamma radiation. The data collected is examined to determine whether useful...
Calcium activated proteases, or calpains, are activated in cardiac muscles under conditions of ischemia/reperfusion and hypoxia. Their activity in skeletal muscle under similar conditions is poorly understood. We tested the hypothesis that hypoxia elevates calpain-mediated proteolysis in isolated glycolytic (extensor digitorum longus, EDL) and oxidative (soleus, SOL) mouse muscles studied...
This study was undertaken to determine if strontium chloride could be used to create a trans-generational otolith mark in steelhead (Oncorhynchus mykiss). I completed two strontium injection trials and a survey of juvenile steelhead from various steelhead hatcheries. The two trials measured Sr:Ca ratios in otoliths in response to injections...
High hydrostatic pressure (HHP) affects the pH of weak acids due to an increased degree of acid dissociation while under pressure. The temporary pH reduction by high pressure in such a case may influence the barotolerance of microorganisms. The objective of this study was to determine the impact of weak...
This report presents progress accomplished during the 20-month period from 1 July 1972 through 31 March 1974 as part of the program "Ecological Studies of Radioactivity in the Columbia River Estuary and Adjacent Pacific Ocean". This program is a continuing study supported by the Division of Biomedical and Environmental Research...
Nitrosomonas europaea (ATCC19718) participates in the biological removal of nitrogen from wastewater by oxidizing ammonia to nitrite, the first step in nitrification. This bacterium is sensitive to heavy metals (Zn²⁺, Cd²⁺, Cu²⁺, and Hg²⁺) and cyanide, compounds commonly encountered in wastewater treatment plants. Therefore, we characterized physiological and transcriptional responses...
Zinc is an essential mineral that is integral to many proteins and transcription factors that regulate key cellular functions. The normal prostate accumulates high concentrations of zinc; however, malignant tissues have significantly lower zinc levels. This unique relationship between zinc and the prostate has sparked interest in the role of...
Chlordecone belongs to the class of persistent organochlorine pesticides that are remarkably resistant to environmental degradation. Even though their use was banned in the United States in 1978, these compounds can still be detected in both humans and wildlife throughout the world. Previous work has shown that the pretreatment of...
Summer mortality of farmed Pacific oysters causes financial losses for shellfish growers, and selective breeding to improve survival of offspring is a promising way to reduce losses. This dissertation has two objectives to assist the development of tools for use in selective breeding programs.
Objective 1: Identify candidate genes for...
Heparin was modified and immobilized to surface-activated silica surfaces using two different reaction schemes. End-aminated heparin was reacted with 2-iminothiolane to produce free thiol groups at the terminal ends of the heparin chains. The end-thiolated heparin was immobilized by reaction with a pyridyl disulfide activated poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] triblock...
Recently, changes in human performance following whole-body vibration (WBV) training have been attributed to enhanced neuromuscular function. However, the exact neural and muscular mechanisms responsible for these changes remain less understood. The purpose of this study was to evaluate the acute and chronic effects of whole body vibration on the...
In the presence of internal or external stressors, the body requires sources of energy that aide cells to combat inflammation. Fatty acids are an important source of energy and are vital components of cell membranes. Dietary fatty acids (n-6 and n-3) are of importance in immune function because they are...
Chlorine – 36 (36Cl) is produced naturally, through neutron activation of stable chlorine during nuclear weapons detonation, or from neutron capture on residual chlorine in graphite piles. It has a half-life of 301,000 years and decays by the means of beta decay with an average energy of 236.33 keV. Due...
Acute toxicity bioassays (96-hr TL₅₀) were employed to determine
the tolerance of coho salmon (Oncorhynchus kisutch) to inorganic
chloramines at the alevin (yolk sac fry), yolk absorption, and early
juvenile life stages. Any influence of changes in temperature, pH,
and total alkalinity on the tolerance of juvenile coho for chloramines...
Oxidative stress that occurs during cryopreservation may be reduced by the addition of antioxidants. Vitamin E (Vit E), Vitamin C (Vit C), glutathione (GSH), lipoic acid (LA), glycine betaine (GB) and polyvinylpyrrolidone (PVP) were applied at four steps of the PVS2 vitrification protocol (pretreatment, loading, rinsing, and regrowth). Shoot tips...
Nitrosomonas europaea carries out the first step in the biological nitrogen removal process in wastewater treatment by oxidizing ammonia to nitrite. It has been shown to be sensitive to a wide range of contaminants. The antibiotic oxytetracycline and the heavy metals chromium and silver have been found in wastewater influent....
A series of studies, in three western Oregon Douglas-fir plantations, was conducted to understand the physiological impacts of Swiss needle cast on Douglas-fir physiology. Four aspects of the disease complex were investigated: fungal colonization and assessment, plant-water relations, carbon assimilation and interaction with climate. Several techniques were developed and used...
Adula californiensis, which inhabits mudstone along the open
coast and in Yaquina Bay, Oregon, is subjected to extremely varying
conditions. Laboratory experiments of a factorial design were used
to investigate the effects of temperature and salinity on survival,
growth and respiration during early development.
The rate of development to the...
This dissertation presents a research series demonstrating the use of pharmacokinetic modeling and simulations as tools to assess drug concentration and disposition in patient populations. For drugs requiring therapeutic drug monitoring, these tools are necessary to ensure patients are receiving a safe and effective dose of medication to address their...
This research explores the viability and effectiveness of using an inertial navigation system (INS) to control a beamforming array of microstrip patch antennas with the aim of reducing users' exposure to electromagnetic radiation. The system reduces radiated power directed toward a cellular phone user to below 10% of the total...
Published October 1966. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog
Studies of physiological effects of fungicides on fungi are critical for understanding
modes of action and mechanism of resistance; however, studies are difficult to perform
on filamentous fungi because of the inability to produce homogeneous a biological
system using a mass of mycelium. Moreover, the presence of rigid cell wall...
Published July 1966. Facts and recommendations in this publication may no longer be valid. Please look for up-to-date information in the OSU Extension Catalog: http://extension.oregonstate.edu/catalog
A study on the effects of light quality and periodicity on the germination of Douglas-fir seed and the growth of Douglas-fir seedlings was conducted. Interest was focused on responses that could be measured and observed without biochemical assay. It was found that .Douglas-fir seed,s would respond to red-light treatments by...
Concentrations of twenty-five PCBs, fifteen organochiorine pesticides and mercury were determined in recreational fish from the Willamette River, Oregon during the summer of 2000. Thirty-six fish samples of three fish species including black crappie, smalimouth bass and con-rn-ion carp were analyzed. The data reported here provides new information and recent...
The seasonal distribution of bioavailable organochiorine contaminants in surface
water and the potential environmental factors influencing their bioavailability were
evaluated. The study was carried at the lower Willamette River at Portland Harbor, Oregon
where surface water runoff varied according to season. Bioavailable water concentrations of
DDTs and PCBs were determined...