Microbial activity within elevated CO2 and oxygen depleted environments changes with
density driven stratification overturn in the seasonally anoxic region of Devil’s Hole,
Bermuda. The temperature gradient developed during the summer months creates a
natural laboratory to study bacterial and virus population density and microbial
community dynamics in the anoxic...
Over 100 monthly bacterioplankton DNA samples, from each of the surface and 200 m depths at the Bermuda Atlantic Time-series Study (BATS) site, were analyzed for community assembly processes. Correlation networks, filtered for potential autocorrelation artifacts, were constructed for each depth. Network characteristics for the two depths were remarkably similar...
In recent years, the general public has recognized the dangers of a high fat diet and
are demanding meat with lower fat content. This demand has stimulated research in the
growth and regulation of adipocytes. However, despite much effort, no adipocyte-specific
plasma membrane markers from any species are available as...
Iron (Fe) and copper (Cu) are essential cofactors for microbial metalloenzymes, but little is known about the metalloenyzme inventory of anaerobic marine microbial communities despite their importance to the nitrogen cycle. We compared dissolved O₂ , NO₃⁻, NO₂⁻, Fe and Cu concentrations with nucleic acid sequences encoding Fe and Cu-binding...
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, Stephen J. Giovannoni 7, KevinL. Vergin 7 and Frank J. Stewart 1, 2
1 School of Earth and Atmospheric
Iron (Fe) and copper (Cu) are essential cofactors for microbial metalloenzymes, but little is known about the metalloenyzme inventory of anaerobic marine microbial communities despite their importance to the nitrogen cycle. We compared dissolved O₂ , NO₃⁻, NO₂⁻, Fe and Cu concentrations with nucleic acid sequences encoding Fe and Cu-binding...
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J.
Giovannonig, KevinL. Verging, Frank J. Stewarta,b
*Correspondence: Corresponding Author
Iron (Fe) and copper (Cu) are essential cofactors for microbial metalloenzymes, but little is known about the metalloenyzme inventory of anaerobic marine microbial communities despite their importance to the nitrogen cycle. We compared dissolved O₂ , NO₃⁻, NO₂⁻, Fe and Cu concentrations with nucleic acid sequences encoding Fe and Cu-binding...
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reaction center, L and M subunits 81482 0 0 0 629 122 12 10 6 170 6 11 0
f.21.1.2 Cytochrome b of
Inflammation is one of the first, most important responses of the immune system when presented with infection and is mediated primarily by eicosanoids and cytokines. The goal of this research was to generate monoclonal antibodies against murine IL17C, a poorly characterized cytokine involved in inflammation and linked to inflammatory conditions....
This thesis is a collection of four submitted manuscripts that present
methods to assist forest ecosystem service managers wanting to develop
operational sampling, monitoring, and production plans for a set of
specific quantifiable ecosystem services, which are formulated as a
series of general multi-objective optimization problems. The problems
are solved...
In this investigation, six different endogenous thyroid hormone derivatives—T₄, T₃, TA₄, TA₃, T₁-Amine, and N-Acetylated T₁-Amine—were tested to gain insight into their permeability coefficients and efflux ratios across a monolayer of Madin-Darby Canine Kidney (MDCK II) cells. The results indicate that T₃ and T₄ were unable to traverse the membrane...
This study sought to explore the differences between the varied academic advising models in the College of Science at Oregon State University through the feedback of its undergraduate students and advisors. Using parallel surveys of students and their academic advisors, it found that the decentralized and centralized models each had...
Uveitis is a serious ophthalmological disorder characterized by intraocular infiltration of inflammatory cells. In most cases, uveitis is derived from the adaptive immune response. More specifically, CD4+ T lymphocytes play an important role in the pathogenesis of uveitis by recognizing uveitogenic antigen and orchestrating the immune response. While it is...
A one-mile section of a newly constructed forest road in Northwest Oregon was analyzed for various aspects of subgrade and surface strength and their association with the formation of ruts during the first hauling season. Field and laboratory tests were completed on the road and the road materials to determine...
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Kevin Boston
A one-mile section of a newly constructed forest road in Northwest Oregon was
Approximately five to eight percent of domestic rams demonstrate a sexual attraction
towards other rams, directing no sexual behaviors towards estrous ewes. It has been
observed that these male-oriented rams (MORs) have a greater abundance of prolactin
mRNA than female-oriented rams in the medial preoptic area of the anterior
hypothalamus...
Total US lumber production in 2011 was 77.9 million cubic meters. Its primary use was for housing and construction. There is a growing concern that the structural properties for wood are being reduced as trees are harvested at much younger ages as the wood supply shifts from older to younger...
The USDA Forest Services' actions have come under greater scrutiny by a public that has become increasingly concerned with the legality of their actions. Often, challenges involve the quality of science used in the decision-making. Congress has mandated that the agency use the "best available science" in its decision making...
The pulse-coupled oscillator model is widely used to simulate the dynamics of neural systems. For networks with particular internal (Mirollo-Strogatz) dynamics, I will define three broad patterns of collective behaviors found in literature in terms of inter-spike interval (ISI) statistics. These patterns are i) temporally-regular, characterized by all oscillators in...
Iron (Fe) and copper (Cu) are essential cofactors for microbial metalloenzymes, but little is known about the metalloenyzme inventory of anaerobic marine microbial communities despite their importance to the nitrogen cycle. We compared dissolved O₂ , NO₃⁻, NO₂⁻, Fe and Cu concentrations with nucleic acid sequences encoding Fe and Cu-binding...
Our understanding of nitrogen (N) and phosphorus (P) variability in forested stream systems with minimal disturbance is confounded by the complex interactions of hydrologic and biogeochemical controls. In this thesis, our studies evaluate the variability of stream water N and P in a forested, headwater system in the Oregon Coast...
Forest harvest practices can impact nutrient concentrations and stream temperatures, altering aquatic ecosystems. To better inform future sustainable forest resource management practices, quantifying the impact of current practices on water quality, particularly in headwater streams is important. In this study, I quantified monthly nitrate-nitrite (N) and orthophosphate (P) concentrations, 7-day...
In recent decades, the occurrence of large, high severity wildfires has increased substantially in many parts of the world, including the western United States. These fires have the potential to impact water quality, aquatic ecosystems, and downstream communities for many decades. As such, it is increasingly important to understand the...
Work engagement is demonstrated when employees are involved with, committed to,
enthusiastic, and passionate about their work. The goal of this study was to summarize
relevant information on work engagement, produce a working coding system to identify
evidence of work engagement, and discuss the implications of work engagement on an...
The psychoactive component of marijuana, A9-tetrahydrocannabinol (THC), is known
to have effects in humans that are mediated by the cannabinoid receptor (CB 1). Although a great deal of research has been done to confirm the interaction of exogenous cannabinoids with the CB 1 receptor, little is known about the receptor's...
Current natural product (NP) research is limited by its reliance on bioassay-guided fractionation to identify bioactive compounds in mixtures. Computational approaches may improve NP research by correlating mass spectra (MS) and nuclear magnetic resonance spectra (NMR) of bioactive mixtures to their bioactivity patterns. In this study, I use artificial neural...
The extent and severity of wildfires in forested regions are increasing throughout many regions on the planet, including western North America. High-severity wildfires directly affect soils and vegetation by altering soil hydraulic properties, reducing soil organic matter, exporting carbon and nitrogen, and killing trees and understory vegetation. These impacts can...
The 3-substituded indolic enamide moiety has been found in many marine compounds over the past 20 years. These indolic enamides exhibit various biological properties such as cytotoxic, anthelmintic, antimicrobial and HW-inhibitory activities. Among these indolic enamides, some are (E)-enamides, like coscinamide A and coscinamide B, others are (Z)-configuration in their...
Studies toward the total syntheses of highly potent cytotoxic alkaloids
including the bromopyrrole alkaloids and indole alkaloids were conducted and are
described. Studies carried out in the course of this dissertation consist of five total syntheses of natural products that include bromopyrrole alkaloids nagelamide A, D and agelastatin D as...
Shallow subsurface flow and surface runoff are spatially and temporally variable in forested environments. The location and timing of this runoff depends on both site characteristics (e.g., vegetation, soil texture, geology, and topography) and on time-varying conditions (e.g., soil water content, precipitation type, duration, magnitude, and intensity). Forest management activities...
The epoxide deoxygenation reaction is formally the reverse of theepoxidation reaction. Compared to epoxidation, which has reached its fullmaturity, epoxide deoxygenation has not been as intensively developed.Among the few deoxygenation reagents, a handful are catalytic in a metalcomplex, show high stereospecificity and operate under mild conditions. Acommon feature of all...
In situ, reduction of Cp*ReO₃ by PPh₃ to form (Cp*ReO)₂(μ-O)₂ allows catalytic deoxygenation of epoxides, however, conproportionation between Reⱽ and Reⱽᴵᴵ species to form clusters of ((Cp*Re)₃(μ-O)₆}²⁺(ReO₄⁻)2 and new compound {(Cp*Re)₃(μ²-O)₃(μ³-O⁾³ReO₃}⁺(ReO₄⁻) leads to removal of rhenium from the catalytic cycle and loss of activity, The epoxide deoxygenation mediated by (Cp*ReO)₂(μ-O)₂...
In situ reduction of hydrido-tris-(3,5-dimethylpyrazolyl)borato(trioxo) rhenium(V) with triphenylphosphine or triethylphosphite leads to a reactive rhenium(V) species that catalytically deoxygenates epoxides at 75-105°C. The reaction is stereospecific, except for trans- and cis-butene oxide which formed minor amounts of the opposite isomer. A variety of different functional groups were tolerated and even...
A series of compounds, 7-8 and 20-25, were tested as competitive inhibitors of 2,5-dihydroxyacetanilide epoxidase I (DHAE I) and DHAE II. A Hammett plot was constructed for each enzyme to determine the effect of electron density on inhibition. DHAE I gave a linear, highly correlated plot (r²=0.91) that signifies the...
The rhenium (V) complex Tp'ReO₂ (Tp' = hydrido-tris-(3,5-
dimethylpyrazolyl)borate), generated in situ from PPh₃ reduction of
Tp'Re0₃, reacts with small ring heterocycles such as epoxides and
episulf ides. The strained trans-cyclooctene ring provides an unusual
thermodynamic bias against direct atom transfer, but trans-cyclooctene
oxide still produces substantial amounts of trans-cyclooctene....
The gut microbiome has been previously implemented in a number of diseases. Of note is its substantial role in metabolic syndrome, which encapsulates a group of conditions that increase the risk of cardiovascular disease, type 2 diabetes, and stroke. Understanding the causal relationships between the gut microbiome and host processes...
Timber harvesting practices can increase fine sediment inputs to streams due to increased hillslope soil erosion and mass wasting of roads, hillslopes, and stream channels. Excessive fine sediment depositions have been shown to impact aquatic ecosystems, fish habitat, and downstream community water supply. Despite these deleterious impacts, the influence of...
The rhenium dithiodiolate, Hydrido-tris-(3 ,5-dimethyl- 1 -pyrazol yl) borato(ethane- 1 ,2-dithiodiolato)(oxo)rheni um(V), and Hydrido-tris-(3 ,5-dimethyl- 1 -pyrazolyl)borato(phenylethanedithiodiolato)(oxo)rhenium(V), were synthesized by reductive cyclocondensation of alkane-1,2-dithiols with Tp'Re0₃ in a one pot fashion. The rhemium monothiodiolate, Hydrido-tris-(3 ,5-dimethyl- 1 -pyrazolyl) borato(ethane- 1 ,2-monothiodiolato)(oxo)rhenium(V), and Hydrido-tris-(3 ,5- dimethyl- 1 -pyrazolyl)borato(propanemonothiodiolato)(oxo)rhenium(V), were also synthesized...
Airborne discrete-return lidar is an active remote sensing technology capable of obtaining accurate, fine-resolution three-dimensional measurements over large areas. Discrete-return lidar data produce three-dimensional object characterizations in the form of point clouds defined by precise x, y and z coordinates. The data also provide intensity values for each point that...
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Kevin D. Boston
Airborne discrete-return lidar is an active remote sensing technology
Flow cytometry is a highly-extensible technology for performing rapid multi-parametric particle analysis. The use of flow cytometry has proliferated with the advent of smaller, intuitive, and more affordable instruments, along with a perpetual increase in research and medical applications. However, the technology is still largely inaccessible due to cost and...
In this dissertation, a novel method for analyzing computed transition states (TS) for organic reactions is disclosed. It introduces the use of localization-delocalization matrices as parameters in a linear free energy relationship to determine the transition state interactions responsible for differences in energy between the major and minor TS in...
Current frameworks for analyzing forest carbon offset projects in disturbance-prone western forests often fail to address the dynamic nature of carbon pathways through time. They do not account for the probability of loss due to wildfire, which can influence the prediction of carbon storage at the end of a planning...
Wildfires can affect soil physical properties, resulting in changes in infiltration, hillslope runoff, groundwater recharge, and sediment and dissolved nutrients to streams. In November 2016, the Chimney Tops 2 Fire burned 4,617 ha of Appalachian hardwood forest in eastern Tennessee. A portion of the fire burned through a National Ecological...
The purpose of this study was to investigate the effects of wildfire on soil hydraulic properties of forested hillslopes. The study site was located within the Stouts Creek wildfire, which burned approximately 10,705 ha of forest in southwestern Oregon in summer 2015. Three soil hydraulic properties (soil moisture, unsaturated hydraulic...
Headwater streams represent the majority of the drainage area of a river network and provide many critical functions supporting aquatic and terrestrial ecosystem health. The riparian zones that surround these streams are transitional areas between terrestrial and stream ecosystems, which provide key habitat for aquatic and terrestrial organisms, especially during...
Mountainous headwater streams make up ~80 % of stream length globally and are strongly connected with catchment hillslopes and riparian areas, which can influence water quantity, quality, and availability for downstream uses. Accordingly, effective management of headwater streams and riparian zones to maintain desired ecosystem services downstream is critical, particularly...
Forested headwater streams are critical for the supply of water, sediment, nutrients, and organic matter to downstream water bodies. Nearly half of all headwater streams display temporary flow regimes (i.e., non-perennial), but with climate change they may become more common. Thus, it will be increasingly imperative to adequately represent non-perennial...
Skeletal muscles provide several functions, including, yet not limited to, voluntary body movement and locomotion, posture and body position, and soft tissue support. Skeletal muscles also play a role in metabolism, requiring energy to facilitate contraction. Each skeletal muscle serves a different purpose despite seeming to perform the same task....
Cell counting is a key tool used in biological and clinical laboratories, but at present, commercial cell counters are large and expensive. CMOS technology is a promising platform for creating small, low-cost sensors but currently lacks a robust method of directly interfacing these sensors with the microfluidic networks needed for...