The amino acid acridon-2-ylalanine (Acd) can be a valuable probe of protein conformational change because it is a
long lifetime, visible wavelength fluorophore that is small enough to be incorporated during ribosomal biosynthesis. Incorporation
of Acd into proteins expressed in E. coli requires efficient chemical synthesis to produce large quantities...
Full Text:
Fluorescent Amino Acid for Lifetime and
FRET/LRET Studies
Lee C. Speight,† Anand K. Muthusamy,† Jacob M
The amino acid acridon-2-ylalanine (Acd) can be a valuable probe of protein conformational change because it is a
long lifetime, visible wavelength fluorophore that is small enough to be incorporated during ribosomal biosynthesis. Incorporation
of Acd into proteins expressed in E. coli requires efficient chemical synthesis to produce large quantities...
Full Text:
FRET/LRET Studies
Lee C. Speight,† Anand K. Muthusamy,† Jacob M. Goldberg,† John B. Warner,† Rebecca
Basic biological information is stored in strings of nucleic acids (DNA, RNA) or amino acids
(proteins). Teasing out the meaning of these strings is a central problem of modern biology. Matching and
aligning strings brings out their shared characteristics. Although string matching is well-understood in the
edit-distance model, biological strings...
General circulation models (GCMs) predict that the global hydrological cycle will change in response to anthropogenic warming. However, these predictions remain uncertain, in particular, for precipitation (Intergovernmental Panel on Climate Change, 2013, https://doi .org/10.1017/CB09781107415324.004). Held and Soden (2006, https://doi.org/10.1175/JCLI3990.1) suggest that as lower tropospheric water vapor concentration increases in a...
The potent antitumor agent phorboxazole A was synthesized from six subunits comprising C1-C2 (115), C3-C8 (98), C9-C19 (74), C20-C32 (52), C33-C41 (84) and C42-C46 (85). Tetrahydropyrans B and C containing cis-2,6-disubstitution were fabricated via palladium(II)-mediated intramolecular alkoxycarbonylation which, in the case of tetrahydropyran C, was carried out with catalytic palladium(II)...
Lateral stirring is a basic oceanographic phenomenon affecting the distribution of physical, chemical, and biological fields. Eddy stirring at scales on the order of 100 km (the mesoscale) is fairly well understood and explicitly represented in modern eddy-resolving numerical models of global ocean circulation. The same cannot be said for...
The Generation Challenge programme (GCP) is a global crop research consortium directed toward crop improvement through the
application of comparative biology and genetic resources characterization to plant breeding. A key consortium research activity
is the development of a GCP crop bioinformatics platform to support GCP research. This platform includes the...
Internal gravity waves, the subsurface analogue of the familiar
surface gravity waves that break on beaches, are ubiquitous in
the ocean. Because of their strong vertical and horizontal currents,
and the turbulent mixing caused by their breaking, they affect a
panoply of ocean processes, such as the supply of nutrients...
The Gulf Stream is a major conduit of warm surface water from the tropics to the subpolar North Atlantic. Here we observe and simulate a submesoscale (<20 km) mechanism by which the Gulf Stream exchanges water with subpolar water to the north. Along isopycnals, the front has a sharp compensated...