Bacterial cultures enriched from sheep rumen fluid have demonstrated the ability to detoxify pyrrolizidine alkaloids (seneciphylline and jacobine) in tansy ragwort (Senecio jacobaea). The microbes are difficult to isolate using classical anaerobic techniques, therefore, microbes from two different enrichment cultures demonstrating similar degradation activity were identified using their 16S ribosomal...
Rhodococcus fascians is a Gram-positive, pleiomorphic actinomycete that causes leafy gall formation in a wide range of plant hosts. This particular phytopathogen has been a focus of research at the Oregon State University Plant Clinic, a diagnostic laboratory. Phylogeny of R. fascians isolates obtained from Plant Clinic submissions was determined...
Despite knowledge that concerted evolution of high-copy loci is often imperfect,
studies that investigate the extent of intragenomic polymorphisms and comparisons
across a large number of species are rarely made. We present a bioinformatic pipeline
for characterizing polymorphisms within an individual among copies of a high-copy
locus. Results are presented...
Gymnomyces is an ectomycorrhizal basidiomycete genus in the family Russulaceae estimated to contain less than 50 species worldwide, with 23 species known to occur in the Pacific Northwest. Gymnomyces species are common in Pacific Northwest forests that include Pseudotsuga, Abies, Tsuga, and Quercus. They produce sequestrate (truffle-like) sporocarps with ornamented...
Ramaria is a genus of epigeous fungi common to the coniferous forests of the Pacific Northwest of North America. The extensively branched basidiocarps and the positive chemical reaction of the context in ferric sulfate are distinguishing characteristics of the genus. The genus is estimated to contain between 200-300 species and...
Rhizopogon is a hypogeous fungal genus that forms ectomycorrhizae
with genera of the Pinaceae. The greatest number and species of
Rhizopogon are found in coniferous forests of the Pacific Northwestern
United States, where members of the Pinaceae are also concentrated.
Rhizopogon spp. are host-specific primarily with Pinus spp. and
Pseudotsuga...
Investigators have been studying an aggregate
containing enzymes of deoxyribonucleoside triphosphate
biosynthesis in T4-phage infected bacteria. They suggest
that it behaves as an organized complex, efficiently
channeling DNA precursors to the replication apparatus.
Previous work has suggested that about 10 enzyme
activities remain associated through several fractionation
steps. This multi-enzyme...
This dissertation describes an investigation of DNA precursor supply for mitochondrial and nuclear DNA synthesis in HeLa cells and Mouse L-cells. HeLa cells were used for the quantitation of cellular and mitochondrial deoxyribonucleoside triphosphate (dNTP) and ribonucleoside triphosphate (rNTP) pools and of changes in pools in response to treatment with...
A fluorescent dye, 1-dimethylaminonaphthalene-5-sulfonyl
chloride (dansyl chloride), was covalently bonded to polylysine. The
dansyl tagged polylysine was complexed to DNA by gradient dialysis.
The polarization and intensity of fluorescence of the dansyl and the
turbidity of the complex were measured as a function of salt concentration
for a variety of...