Thiamine (vitamin B₁) is required by all living organisms for carbohydrate metabolism and synthesis of amino acids. Thiamine deficiency is responsible for several related classes of early life stage mortality disorders in salmonines, including Thiamine Deficiency Complex (TDC) in the Laurentian Great Lakes, Cayuga Syndrome in the Finger Lakes, and...
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Scott A. Heppell
Thiamine (vitamin B1) is required by all living organisms for carbohydrate
This work sought to characterize a link between Vitamin C and nitroglycerin at the level of enzymatic bioactivation. Before we could proceed, we needed to develop a method that sensitively and selectively identified the product of bioactivation, nitrite. We successfully adapted traditional diaminonaphthalene derivatization with the use of stable isotope...
We compared a kinetic method (KM) and a colorimetric method (CM) for
measuring erythrocyte aspartate aminotransferase (EAST) activity. Twenty-three
healthy college students including 7 men and 16 women, aged from 22 to 40 years,
participated in this study. Vitamin B-6 status was assessed by EAST activity
coefficient (EAST-AC), the ratio...
The effect of pyridoxine (PN) supplementation on the activities
of erythrocyte alanine aminotransferase (EAlaAT) and aspartate aminotransferase
(EAspAT) was observed in five men, aged 22 to 25 years.
The subjects received a constant diet containing 1.34 mg of vitamin
B-6 Monday through Friday of each week during this five-week study....
The effect of vitamin B-6 (B-6) status on plasma fatty acids (FA) levels and lipid
metabolism was investigated in this metabolic study. Eight female subjects were fed for 28
days. For the first 7 days, they were fed a constant diet containing 2.10 mg of B-6. For the
rest of...
This study examined the effect of vitamin B-6 supplementation and exhaustive
submaximal exercise on plasma catecholamine concentrations, and the relationship
between plasma catecholamines and fuel use, heart rate and oxygen consumption. Five
trained men (age= 18-35 years; V0₂max=53 ml 0₂/kg/min.) participated in two controlled
dietary periods that were identical except...
Trained male cyclists (6 in study 1, 5 in study 2) cycled to exhaustion (EXH) at
75% of VO₂ max twice; once in the non-supplemented (NS) state and once in the vitamin
B-6 (B-6)(20 mg PN) supplemented (S) state. The diet contained 2.3 mg B-6 in study 1
and 1.9...
Serine transhydroxymethylase (STHM), a pyridoxal 5'-
phosphate requiring enzyme is indirectly involved in
pyrimidine deoxynucleotide metabolism. A decrease in the
activity of this enzyme could lead to altered deoxycytidine
(dC) metabolism. This study was undertaken to determine if
a vitamin B-6 deficiency affects dC metabolism. The effect
of a vitamin...