Cryopreservation of adherent cells may be advantageous for cell types that are difficult to
preserve in suspension or when it is necessary to preserve characteristics of the adherent cultured cells. Vitrification is a promising procedure for the preservation of adherent cells that prevents ice crystal formation and the resulting dissociation...
BACKGROUND: Simple and effective cryopreservation of human oocytes would have
an enormous impact on the financial and ethical constraints of human assisted
reproduction. Recently, studies have demonstrated the potential for cryopreservation
in an ice-free glassy state by equilibrating oocytes with high concentrations of
cryoprotectants (CPAs) and rapidly cooling to liquid...
Contact of blood with the surfaces of synthetic materials is associated with spontaneous protein adsorption, initiating platelet aggregation, the coagulation cascade, and the eventual development of a stable clot. Current therapy to inhibit implant-induced thrombosis is life-long administration of systemic anticoagulants. An alternative to the systemic administration of anticoagulant drugs...
Ice-free cryopreservation, known as vitrification, is an appealing approach for banking of adherent cells and tissues because it prevents dissociation and morphological damage that may result from ice crystal formation. However, current vitrification methods are often limited by the cytotoxicity of the concentrated cryoprotective agent (CPA) solutions that are required...
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Cryoprotectant Addition and Removal Procedures for Adherent
Endothelial Cells
AllysonFryDavidson, Cameron
Ice-free cryopreservation, known as vitrification, is an appealing approach for banking of adherent cells and tissues because it prevents dissociation and morphological damage that may result from ice crystal formation. However, current vitrification methods are often limited by the cytotoxicity of the concentrated cryoprotective agent (CPA) solutions that are required...
Full Text:
Endothelial Cells
AllysonFry Davidson1, Cameron Glasscock1, Danielle R. McClanahan1, James
D. Benson2, Adam Z
Ice-free cryopreservation, known as vitrification, is an appealing approach for banking of adherent cells and tissues because it prevents dissociation and morphological damage that may result from ice crystal formation. However, current vitrification methods are often limited by the cytotoxicity of the concentrated cryoprotective agent (CPA) solutions that are required...
This project-based portfolio is the capstone of my work in Oregon State University's College Student Services Administration (CSSA) Master’s in Education program and is composed of three components: (1) an Online Advising Course for students applying to competitive national and international scholarships, (2) a reflective essay that synthesizes my learning...
Vitrification is a promising approach for cryopreservation of adherent cells because it allows complete avoidance of ice formation. However, high cryoprotectant (CPA) concentrations are required to prevent freezing, and exposure to high CPA concentrations increases the risk of osmotic and toxic damage. Although cell membrane transport modeling can be used...
The objective of this study was to compare the effects on eating behavior,
lipids, lipoproteins, lipid peroxidation, and glycemic control in women with type 2
diabetes of a high-monounsaturated fat diet (HM) compared to a high-carbohydrate
diet (HC).
In an outpatient feeding study, ten hypertriglyceridemic postmenopausal type
2 diabetic women...
M. E. Hard defines a mushroom as "a cellular,
flowerless plant, nourished by the mycelium which permeates
the soil or other substances on which the mushroom
grows".
In all probability mushrooms are the most, interesting of all the higher organized fungi. Within the past few years, interest in them has...