Potato Virus Y (PVY) is an enduring problem for potato production worldwide. Potato varieties that resist the “ordinary” strain PVYO were developed by breeders, but in recent years, recombinant strains of PVY have been able to circumvent this strain-specific resistance and evade growers and seed certification officials by causing fewer...
Two research projects comprise this thesis. The first project investigated modifying the hormone dosing strategy traditionally used in superovulating donor cows for an embryo collection to decrease drug costs without decreasing the number of high quality, transferable embryos recovered. The objective of this project was to evaluate the number and...
Bcl11b (B-cell lymphoma/leukemia 11b), also known as Ctip2 (Chicken ovalbumin upstream promoter transcription factor (COUP-TF)-interacting protein 2), is a C2H2 zinc finger transcriptional regulatory protein, which is an essential protein for post-natal life in the mouse and plays crucial roles in the development, and presumably function, of several organ systems,...
Curbing overall disease burden with faster intervention requires early stage and accurate diagnostics. Ultrasound (US) provides a safe, nonionizing, inexpensive, and noninvasive imaging modality that has become commonplace in diagnostics. Paired with the relatively new technology of photoacoustic imaging (PAI), optical level resolution becomes feasible with the deeper signal penetration...
The objective of the present research was to determine, by use of the progesterone receptor antagonist RU-486, whether progesterone by autocrine/paracrine action suppresses phosphatidylinositol hydrolysis, and thus, the production of prostaglandin F2a during the midluteal phase of the estrous cycle in ewes. Two experiments were performed. Experiment 1 consisted of...
Hydrogen peroxide quantification is of broad interest due to the common use of hydrogen peroxide as an oxidizing agent in industrial processing and laboratory research. Hydrogen peroxide assays are also of general importance for biological studies aimed at understanding the role of in situ generated reactive oxygen species. In the...
The spatial and temporal patterning of sequence specific transcription factors (SSTFs) contributes to cell type specification and organ formation during embryogenesis. Homeodomain transcription factors are evolutionally conserved among invertebrate and vertebrate animals. They are responsible for body segmentation and organogenesis. Lbx1 and Pitx2 both are homeodomain transcription factors contributing to...
Molecular genetic and enzymological techniques have been employed to study secondary metabolite biosynthesis. These investigations have focused on two projects: the cloning and heterologous expression of biosynthetic gene clusters from unculturable marine organisms and the characterization of individual enzymes involved in the biosynthesis of the antifungal agent blasticidin S. The...
The immune system is a complex network of cellular processes working in harmony to guard us against pathogenic attack. Of utmost importance is the ability of the immune system to discriminate between “self” and “non-self” and diseased versus healthy. Recognition of antigens complexed to major histocompatibility complex (MHC) molecules is...
Cognitive impairment, or cognitive decline, a noticeable and measurable decline in cognitive abilities (e.g. memory and learning) that exceeds those attributed to normal aging, represents an early symptom of neurodegeneration and increased risk for progression to more severe dementias, such as Alzheimer's disease (AD). While the complex etiology of these...