The human pathogen Chlamydia trachomatis displays different phenotypes between serovars both in vivo and in vitro. The study of chlamydial biology has been hampered by the lack of a tractable genetic system. Here, chlamydial isolates with known phenotypes as well as strains generated though recombination were fully sequenced to address...
Background: Pre-genomic and post-genomic studies demonstrate that chlamydiae actively recombine in vitro and
in vivo, although the molecular and cellular biology of this process is not well understood. In this study, we
determined the genome sequence of twelve Chlamydia trachomatis recombinants that were generated in vitro
under antibiotic selection. These...
The genome of a herpesvirus highly pathogenic to rabbits, leporid herpesvirus 4 (LHV-4), was analyzed using high-throughput DNA sequencing technology and primer walking. The assembled DNA sequences were further verified by restriction endonuclease digestion and Southern blot analyses. The total length of the LHV-4 genome was determined to be about...
The Lecanoromycetes is the largest class of lichenized Fungi, and one of the most species-rich classes in the
kingdom. Here we provide a multigene phylogenetic synthesis (using three ribosomal RNA-coding and two
protein-coding genes) of the Lecanoromycetes based on 642 newly generated and 3329 publicly available
sequences representing 1139 taxa,...