Vibrio marinus MP-1, an obligate psychrophilic marine
bacterium, was severely damaged when heat-shocked in the presence
of nutrients. Thermally induced leakage materials from cells tested
for in the medium were 260 mμ absorbing material (nucleic acids),
orcinol reacting material (RNA), ninhydrin reacting material (amino
acids), protein, malic dehydrogenase, and glucose-6-phosphate...
The physiological responses of populations of a marine
Pseudomonas sp. to nutrient starvation were observed for a period
of 40 days. During the first 25 days of starvation the viability
of the population decreased by 99.9 percent, but thereafter the
cultures maintained approximately 5 x 10⁵ viable cells per ml...
Psychrotrophic Kanagawa positive marine vibrios distinct
from V. parahaemolyticus were isolated from shellfish
collected in Yaquina Bay, Oregon. Sample enrichment in
GSTB was followed by culture isolation on TCBS agar. The
235 vibrio isolates obtained were screened for gram reaction
and morphology, Kanagawa reaction on Wagatsuma agar
and their response...
The starvation-survival patterns of 16 freshly isolated open
ocean bacteria and in a psychrophilic Vibrio sp., Ant-300, were
determined. These isolates displayed three survival patterns as
determined by viable cell count. These were (a) a rapid increase
followed by a decrease to a constant value (pattern of Ant-300 and
7...
Cells of an obligately psychrophilic marine bacterium, Vibrio
marinus, strain MP-1, were grown at 4 C and 15 C and studied for
possible physiological differences. No differences were noted in
sugar fermentation or temperature-shift growth studies; in both cases
adequate nutrients were available. Some differences were noted in
viability retention...
Washed cells of Vibrio marinus MP-1 grown at 15 C (organism's
optimum growth temperature) were employed in this study. These
cells were exposed to heat at and above their maximal growth temperature
(20 C) for one hour and tested manometrically at 15 C for their
ability to take up oxygen....
The addition of catalase to culture medium increased the period
of viability of Beggiatoa from one week to two months. Addition of
catalase also produced a marked increase in cell yield and enzyme
activity. The addition of cysteine or hydrogen sulfide to the growth
medium or the use of semi-solid...
Vibrio marinus MP-1 (ATCC 15381) deaminated nine of seventeen
amino acids tested with L-glutamine being deaminated to the greatest
and L-serine to the second greatest extent in one hour. The optimum
pH for the L-serine deamination was 8.4.
The response of washed cells to temperature on the deamination
of L-serine...