Rat liver microsomes are shown to contain an enzyme system
which converts the cyclodiene insecticides, aldrin, heptachlor and
isodrin to their corresponding epoxides, dieldrin, heptachlor epoxide,
and endrin. The microsomal fraction of 450 milligrams of liver from
male rats epoxidates 80 percent of a 20 to 50 microgram aldrin substrate...
In vitro studies of Mobam (4-benzo(b)thienyl N-methylcarbamate) metabolism have demonstrated that at least three ether soluble metabolites are formed in a microsomal system fortified with reduced triphosphopyridine nucleotide (NADPH). One of the metabolites was identical to 4HBT (4-hydroxy benzothiophene), the phenol of Mobam. The other two metabolites did not possess...
In vitro studies of Mobam (4-benzo(b)thienyl N-methylcarbamate)
metabolism have demonstrated that at least three ether soluble
metabolites are formed in a microsomal system fortified with reduced
triphosphopyridine nucleotide (NADPH). One of the metabolites was
identical to 4HBT (4-hydroxy benzothiophene), the phenol of Mobam.
The other two metabolites did not possess...
Assay conditions and analytical methods established for the
study of epoxidation of the insecticides aldrin and heptachlor, by
rat liver microsomes have been employed to study the same reaction
catalyzed by trout liver microsomes. At 37° C and pH 8.0, rainbow
trout liver microsomes, incubated with 54.8 millimicromoles of
either...
The polyunsaturated fatty acids (PUFA) are known to be required
for larval growth and normal wing development in several
species of Lepidoptera but most of the basic information necessary
for a complete understanding of this nutritional phenomenon has not
been gathered. This includes the role of each of the essential...
Endogenous inhibitors of insect microsomal oxidases are a serious
problem in in-vitro studies of insecticide metabolism.
Their number,
chemical nature, modes of action, and their effectiveness as inhibitors
are unknown.
In the case of house flies, there may be as many as
four inhibitors.
The most important of these is...
The carboxylesterases of three species of diptera, the flesh
fly (Sarcophaga bullata, Park.), the black blow fly (Phormia regina,
Meig.), and the house fly (Musca domestica, L.) were compared in relation
to hydrolytic activity against three substrates, response to
inhibition and induction, isozyme composition, and age-dependent
variation in activity. Similar...