In Vivo Quantification Reveals Extensive Natural Variation in Mitochondrial Form and Function in Caenorhabditis briggsae Public Deposited

http://ir.library.oregonstate.edu/concern/defaults/bc386k30s

This is the publisher’s final pdf. The published article is copyrighted by the Public Library of Science and can be found at:  http://www.plosone.org/home.action.

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • In We have analyzed natural variation in mitochondrial form and function among a set of Caenorhabditis briggsae isolates known to harbor mitochondrial DNA structural variation in the form of a heteroplasmic nad5 gene deletion (nad5 Delta) that correlates negatively with organismal fitness. We performed in vivo quantification of 24 mitochondrial phenotypes including reactive oxygen species level, membrane potential, and aspects of organelle morphology, and observed significant amongisolate variation in 18 traits. Although several mitochondrial phenotypes were non-linearly associated with nad5D levels, most of the among-isolate phenotypic variation could be accounted for by phylogeographic clade membership. In particular, isolate-specific mitochondrial membrane potential was an excellent predictor of clade membership. We interpret this result in light of recent evidence for local adaptation to temperature in C. briggsae. Analysis of mitochondrial-nuclear hybrid strains provided support for both mtDNA and nuclear genetic variation as drivers of natural mitochondrial phenotype variation. This study demonstrates that multicellular eukaryotic species are capable of extensive natural variation in organellar phenotypes and highlights the potential of integrating evolutionary and cell biology perspectives.
Resource Type
DOI
Date Available
Date Issued
Citation
  • Hicks KA, Howe DK, Leung A, Denver DR, Estes S (2012) In Vivo Quantification Reveals Extensive Natural Variation in Mitochondrial Form and Function in Caenorhabditis briggsae. PLoS ONE 7(8): e43837. doi:10.1371/journal.pone.0043837
Academic Affiliation
Series
Keyword
Rights Statement
Funding Statement (additional comments about funding)
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Deborah Campbell(deborah.campbell@oregonstate.edu) on 2013-02-12T18:19:12Z (GMT) No. of bitstreams: 3 license_rdf: 22765 bytes, checksum: 56265f5776a16a05899187d30899c530 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) HoweDanaKZoologyInVivoQuantification.pdf: 701422 bytes, checksum: 0aa08d56628141e042a038a5bfb70776 (MD5)
  • description.provenance : Submitted by Deborah Campbell (deborah.campbell@oregonstate.edu) on 2013-02-12T17:45:00Z No. of bitstreams: 3 license_rdf: 22765 bytes, checksum: 56265f5776a16a05899187d30899c530 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) HoweDanaKZoologyInVivoQuantification.pdf: 701422 bytes, checksum: 0aa08d56628141e042a038a5bfb70776 (MD5)
  • description.provenance : Made available in DSpace on 2013-02-12T18:19:12Z (GMT). No. of bitstreams: 3 license_rdf: 22765 bytes, checksum: 56265f5776a16a05899187d30899c530 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) HoweDanaKZoologyInVivoQuantification.pdf: 701422 bytes, checksum: 0aa08d56628141e042a038a5bfb70776 (MD5) Previous issue date: 2012-08-28

Relationships

In Administrative Set:
Last modified: 07/17/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items