Targeted Sequence Capture Provides Insight into Genome Structure and Genetics of Male Sterility in a Gynodioecious Diploid Strawberry, Fragaria vesca ssp. bracteata (Rosaceae)

Tennessen, Jacob A.; Govindarajulu, Rajanikanth; Liston, Aaron; Ashman, Tia-Lynn

Citeable URL: https://ir.library.oregonstate.edu/concern/articles/f7623d65n

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Creator	Tennessen, Jacob A. Govindarajulu, Rajanikanth Liston, Aaron Ashman, Tia-Lynn
Abstract	Gynodioecy is a sexual system wherein females coexist with hermaphrodites. It is of interest not only because male-sterile plants are advantageous in plant breeding but also because it can be a crucial step in the evolutionary transition to entirely separate sexes (dioecy) from a hermaphroditic ancestor. The gynodioecious diploid wild strawberry, Fragaria vesca ssp. bracteata (Rosaceae), is a member of a clade with both dioecious and cultivated species, making it an ideal model in which to study the genetics of male sterility. To create a genetic map of F. v. ssp. bracteata, we identified informative polymorphisms from genomic sequencing (3-5x coverage) of two outbred plants from the same population. Using targeted enrichment, we sequenced 200 bp surrounding each of 6575 polymorphisms in 48 F1 offspring, yielding genotypes at 98% of targeted sites with mean coverage >100x, plus more than 600-kb high-coverage nontargeted sequence. With the resulting linkage map of 7802 stringently filtered markers (5417 targeted), we assessed recombination rates and genomic incongruities. Consistent with past work in strawberries, male sterility is dominant, segregates 1:1, and maps to a single location in the female. Further mapping an additional 55 offspring places male sterility in a gene-dense, 338-kb region of chromosome 4. The region is not syntenic with the sex-determining regions in the closely related octoploids, F. chiloensis and F. virginiana, suggesting either independent origins or translocation. The 57 genes in this region do not include protein families known to control male sterility and thus suggest alternate mechanisms for the suppression of male function. Keywords: trait mapping, sex chromosome, pollen, translocations, sex determination
Resource Type	Article
DOI	https://doi.org/10.1534/g3.113.006288
Date Available	2013-09-18T18:10:37+00:00
Date Issued	2013-08-01
Citation	Tennessen, J. A., Govindarajulu, R., Liston, A., & Ashman, T. (2013). Targeted sequence capture provides insight into genome structure and genetics of male sterility in a gynodioecious diploid strawberry, fragaria vesca ssp. bracteata (rosaceae). G3 (Bethesda, Md.), 3(8), 1341-1351. doi:10.1534/g3.113.006288
Journal Title	G3: Genes Genomes Genetics
Journal Volume	3
Journal Issue/Number	8
Academic Affiliation	Integrative Biology
权利声明	In Copyright
Funding Statement (additional comments about funding)	This work was supported by the National Science Foundation to T.L.A. (DEB 1020523) and A.L. (DEB 1020271).
Publisher	Genetics Society of America
Peer Reviewed	Yes
Language	English [eng]
Replaces	http://hdl.handle.net/1957/42641

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Targeted Sequence Capture Provides Insight into Genome Structure and Genetics of Male Sterility in a Gynodioecious Diploid Strawberry, Fragaria vesca ssp. bracteata (Rosaceae)

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Targeted Sequence Capture Provides Insight into Genome Structure and Genetics of Male Sterility in a Gynodioecious Diploid Strawberry, Fragaria vesca ssp. bracteata (Rosaceae)

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