High-Throughput Sequencing of Arabidopsis microRNAs : Evidence for Frequent Birth and Death of MIRNA Genes Public Deposited

http://ir.library.oregonstate.edu/concern/defaults/j38607588

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • In plants, microRNAs (miRNAs) comprise one of two classes of small RNAs that function primarily as negative regulators at the posttranscriptional level. Several MIRNA genes in the plant kingdom are ancient, with conservation extending between angiosperms and the mosses, whereas many others are more recently evolved. Here, we use deep sequencing and computational methods to identify, profile and analyze non-conserved MIRNA genes in Arabidopsis thaliana. 48 nonconserved MIRNA families, nearly all of which were represented by single genes, were identified. Sequence similarity analyses of miRNA precursor foldback arms revealed evidence for recent evolutionary origin of 16 MIRNA loci through inverted duplication events from protein-coding gene sequences. Interestingly, these recently evolved MIRNA genes have taken distinct paths. Whereas some non-conserved miRNAs interact with and regulate target transcripts from gene families that donated parental sequences, others have drifted to the point of non-interaction with parental gene family transcripts. Some young MIRNA loci clearly originated from one gene family but form miRNAs that target transcripts in another family. We suggest that MIRNA genes are undergoing relatively frequent birth and death, with only a subset being stabilized by integration into regulatory networks
Resource Type
DOI
Date Available
Date Issued
Citation
  • Fahlgren N, Howell MD, Kasschau KD, Chapman EJ, Sullivan CM, et al (2007) High-Throughput Sequencing of Arabidopsis microRNAs: Evidence for Frequent Birth and Death of MIRNA Genes. PLoS ONE 2(2): e219. doi:10.1371/journal.pone.0000219
Academic Affiliation
Series
Rights Statement
Publisher
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Made available in DSpace on 2011-02-15T02:06:10Z (GMT). No. of bitstreams: 1 pone.0000219.pdf: 703571 bytes, checksum: 842124fe5b128c11dfc234494f9e356a (MD5) Previous issue date: 2007-02
  • description.provenance : Submitted by Mary Phan (mpscanner@gmail.com) on 2011-02-04T18:41:39Z No. of bitstreams: 1 pone.0000219.pdf: 703571 bytes, checksum: 842124fe5b128c11dfc234494f9e356a (MD5)
  • description.provenance : Approved for entry into archive by Sue Kunda(sue.kunda@oregonstate.edu) on 2011-02-15T02:06:10Z (GMT) No. of bitstreams: 1 pone.0000219.pdf: 703571 bytes, checksum: 842124fe5b128c11dfc234494f9e356a (MD5)

Relationships

Parents:

This work has no parents.

Last modified

Downloadable Content

Download PDF

Items