Graduate Thesis Or Dissertation
 

Applied and basic aspects of prerigor pressurization of bovine skeletal muscle

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  • Investigations were conducted to determine the effect of prerigor pressurization (15,000 psi, 37°C, 2 min.) of beef muscle on the subsequent retail/case-life characteristics of the treated meat. In addition, the activity of the calcium activated factor (CAF) of prerigor pressurized (PRP) muscle was also investigated. For fresh beef samples, PRP significantly (P<0.05) improved the lean color of steaks and also showed less discoloration than those of the control (CON). However, the PRP and CON steaks had similar values for off-odor, bacterial counts and weight loss. No significant differences were observed in any of the above traits for the ground beef samples. For beef samples stored at -1.6±1°C for 0, 3, 5, and 7 weeks, lean color scores of the PRP steaks were consistently higher than the CON at all storage intervals. PRP steaks also showed less discoloration and had higher overall desirability ratings at all storage intervals. Although PRP and CON steaks had similar microbial counts after 0 and 3 weeks of storage, the CON steaks had higher microbial counts after 5 and 7 weeks of storage. PRP had no apparant detrimental effects on the storage or case-life characteristics of meat, but rather it beneficially influenced most of the parameters examined. The effect of PRP on CAF activity was studied to further elucidate the mechanism of meat tenderization by PRP. Crude CAF was prepared from PRP and CON muscles at 0, 1, and 3 days of postmortem storage. Throughout these periods, PRP samples had consistently lower total CAF activity than CON, yet, it seems likely that PRP caused a transient increase in Ca⁺⁺ sufficient to activate CAF for an initial brief period of time, because 1) early postmortem, PRP muscle showed detectable levels of a 95,000-dalton component presumably produced by CAF; 2) Z-lines from at-death PRP muscles were highly degraded; and 3) during very early postmortem storage, the SDS-electrophoretic patterns of PRP myofibrils resembled those of CAF-treated myofibrils. CAF autolysis may account for the low CAF activity observed in PRP muscles at later postmortem storage times.
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file details KoohmaraieMohammad1984.pdf 2017-08-10 Pubblico Scaricare