Effects of prostaglandin F₂α on neutrophil populations, uterine health and reproductive performance in dairy cows Public Deposited

Effects of prostaglandin F2alpha on neutrophil populations, uterine health and reproductive performance in dairy cows

http://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/5138jh685

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Incidences of uterine infections in dairy cattle are high between parturition and Day 21 postpartum. Dairy cows with uterine infections are at risk for prolonged periods of days open and multiple services before becoming pregnant. Neutrophils are the first wave of immune system defense against uterine contamination. Neutrophil function seems to be mediated by reproductive hormones and good uterine health is related to properly functioning neutrophils. To elucidate the interaction between reproductive hormones, neutrophils and uterine health in dairy cows the objectives of this research were to evaluate: 1) changes in circulating white blood cell populations during the estrous cycle, 2) the effects of prostaglandin F₂[subscript α](PGF₂[subscript α]) on circulating white blood cell populations and 3) the effects of a two-injection PGF₂[subscript α] regimen on uterine neutrophil and bacterial populations and numbers of days open and services per conception. In the first experiment, the effect of stage of the estrous cycle on plasma neutrophil numbers was evaluated. Neutrophils were observed throughout the entire estrous cycle and numbers were greatest (P<0.05) on Day 14 (mid-cycle), when the corpus luteum was the dominant ovarian structure and plasma progesterone was at its acme. In the second experiment, plasma neutrophil numbers were examined in cows after injections of saline or the PGF₂[subscript α] pharmaceutical product, Lutalyse. Compared to saline, numbers of neutrophils were greater (P<0.05) 4 and 8 hr after Lutalyse injection. In the third experiment, neutrophil numbers were examined after injections of saline, Lutalyse or the PGF₂[subscript α] analog, Estrumate. Compared to saline, numbers of neutrophils did not differ (P>0.10) from cows injected with Lutalyse or Estrumate. In the fourth experiment, uterine bacterial populations and numbers of neutrophils were quantified in cows treated with Lutalyse or saline on Days 0 and 14 or 14 and 28 postpartum. Compared to saline, Lutalyse treatment decreased (P<0.05) total bacteria present in the uterus and increased (P<0.05) the number of uterine neutrophils. In experiment five, numbers of days open and services per conception were evaluated in cows treated with Lutalyse or saline on Days 0 and 14 or 14 and 28 postpartum. Compared to saline, Lutalyse decreased days open (154.7 ± 14.1 vs. 120.1 ± 7.9 days, respectively; P<0.05) and services per conception (3.0 ± 0.4 vs. 2.3 ± 0.2 services, respectively; P=0.09). These results suggest PGF₂[subscript α] treatment can increase neutrophil and depress bacterial cell populations in favor of the dairy cow's uterine health and may explain why fertility is improved when PGF₂α is administered early in the postpartum period.
Resource Type
Date Available
Date Copyright
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Academic Affiliation
Non-Academic Affiliation
Keyword
Subject
Rights Statement
Peer Reviewed
Language
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Laura Wilson(laura.wilson@oregonstate.edu) on 2012-01-10T17:06:42Z (GMT) No. of bitstreams: 1 LulayAdrienneM2012.pdf: 385353 bytes, checksum: 96b462bbd17d2022c19e8fe7bf55fecb (MD5)
  • description.provenance : Submitted by Adrienne Lulay (cautleya@onid.orst.edu) on 2012-01-06T00:22:36Z No. of bitstreams: 1 LulayAdrienneM2012.pdf: 385292 bytes, checksum: 228a44c9d9a1ea2a1b10510cc3287d1d (MD5)
  • description.provenance : Rejected by Julie Kurtz(julie.kurtz@oregonstate.edu), reason: Rejecting to change the spelling on Acknowlegements, and change to List of Tables. Once revised, open the item that was rejected. Replace the attached file with the revised file and resubmit. Thanks, Julie on 2012-01-06T22:33:18Z (GMT)
  • description.provenance : Made available in DSpace on 2012-01-10T17:06:42Z (GMT). No. of bitstreams: 1 LulayAdrienneM2012.pdf: 385353 bytes, checksum: 96b462bbd17d2022c19e8fe7bf55fecb (MD5) Previous issue date: 2011-12-14
  • description.provenance : Approved for entry into archive by Julie Kurtz(julie.kurtz@oregonstate.edu) on 2012-01-09T22:50:06Z (GMT) No. of bitstreams: 1 LulayAdrienneM2012.pdf: 385353 bytes, checksum: 96b462bbd17d2022c19e8fe7bf55fecb (MD5)
  • description.provenance : Submitted by Adrienne Lulay (cautleya@onid.orst.edu) on 2012-01-09T21:19:43Z No. of bitstreams: 1 LulayAdrienneM2012.pdf: 385353 bytes, checksum: 96b462bbd17d2022c19e8fe7bf55fecb (MD5)

Relationships

In Administrative Set:
Last modified: 08/08/2017

Downloadable Content

Download PDF
Citations:

EndNote | Zotero | Mendeley

Items