Graduate Thesis Or Dissertation
 

Effect of pre-rigor ultrapressurization on bovine intramuscular connective tissue

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  • Samples of pre-rigor sternomandibularis muscle from ten steers were pressurized at 15,000 psi for two min at 35°C to investigate the effects of pressurization and incubation time on the thermal stability of collagen. Immediately after pressurization, pH of the treated samples (pH 5.71) was significantly lower (P < 0.05) than the control samples (pH 6.85). The amount of labile collagen from the pressurized samples was 14.16% of the total collagen and was significantly (P < 0.05) higher than the control samples (10.24%). Pressurization apparently accelerated the glycolytic enzymes causing a rapid drop in pH and reduced the strength of collagen fibers to allow for an increase in the thermolabile fraction. Pressurized samples showed a significantly greater (P < 0.05) release of enzymes (β-galactosidase, catheptic collagenase, cathepsin C and elastase) from lysosomes than the control samples. The pH of the pressurized homogenates was significantly lower (P < 0.05) than the control samples. The combined effect of the lysosomal enzymes, β-galactosidase and β-glucuronidase, on the ability of collagenase to solubilize collagen of pressurized muscle and intramuscular connective tissue isolated from pressurized samples was determined. It was found that the incubation of pressurized samples with lysosomal enzymes significantly decreased (P < 0.05) the thermal stability of collagen. The results provide strong evidence that lysosomal enzymes reduce the strength of muscle collagen and that pressurization does alter the configurational structure of connective tissue making it more susceptible to enzymatic attack.
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