Graduate Thesis Or Dissertation
 

Comparisons of calicivirus pathotypes using oligonucleotide patterns from two-dimensional gel electrophoresis

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  • Six serotypes of calicivirus (San Miguel Sea Lion Virus types 4,6,7 and 13, Mink Calicivirus and Reptile Calicivirus) were plaque purified, and two-dimensional gel electrophoresis fingerprints, of the ³²P-labeled Ribonuclease T1 digested viral RNA, were performed for each serotype. Fingerprints revealed several oligonucleotides with similar mobilities, and therefore, possibly similar base composition. Each fingerprint revealed what was believed to be Poly A of homogeneous size, and Poly C which migrated a different distance for each serotype. For those viruses studied, there was a direct relationship between plaque size and the length of the Poly C tract. This correlation between plaque size and Poly C migration may serve as a valid indicator for determining pathogenic potential of caliciviruses.
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