Graduate Thesis Or Dissertation
 

Studies on the methodology of crossing and selfing in Lolium multiflorum

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  • Studies were conducted to determine the effectiveness of a biological pollen barrier of rye, Secale cereale, in preventing out-crossing between Lolium multiflorum genotypes in each of the 300 crossing blocks established in this experiment. Fluorescent tests of seeds of open-pollinated and single-crossed progeny of four annual ryegrass sources were used to determine the effectiveness of the rye barrier in preventing crossing between crossing blocks. The average percentage of fluorescence of open-pollinated progeny in 30 crossing blocks containing supposedly only non-fluorescing plants was 16.3 percent. The open-pollinated and single-crossed progeny in 46 other crossing blocks were highly correlated and did not differ significantly from each other in percent fluorescence. The percent fluorescence of each crossing block was mainly due to intra-crossing block pollen rather than inter-crossing block or foreign pollen. The results indicate that an eight foot barrier of rye is effective in preventing crossing between the annual ryegrass crossing blocks. Self-fertility of Lolium multiflorum was generally low, but self fertility estimates ranged from 0 to 87 percent. The results revealed that self-fertility data obtained by germination rather than seed count may give more accurate self-fertility estimates, especially when individual plants and their differences are being investigated. The average seed set under white parchment bags was not significantly lower than the average seed set obtained with bag-type dialysis tubing. The use of dialysis tubing as a substitute for white parchment bags in bagging plants would be of little, if any, benefit to improve seed set under bag in Lolium multiflorum.
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file details BergmanJeraldW1968.pdf 2017-08-23 Pubblico Scaricare