Honors College Thesis
 

Inclusion development in cells infected with Chlamydia abortus

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https://ir.library.oregonstate.edu/concern/honors_college_theses/1v53k4747

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  • Chlamydia abortus, an intracellular bacterium, spreads through flocks of sheep and goats, causing ovine enzootic abortion (OAE). Chlamydiae develop within a pathogen-modified vacuole in the host cell called the inclusion, which allows the bacteria to acquire host resources. The morphology of the inclusion varies between chlamydial species. Some species fuse multiple inclusions together, others maintain separate inclusions when infected with multiple bacteria. Likewise, some inclusions from different species can fuse in coinfected cells. Our two aims were to determine if C. abortus and closely related C. caviae inclusions can fuse, and if C. abortus inclusions fuse homotypically in single infections. These aims were to determine the fusogenic properties of C. abortus. To address these questions, we singly or doubly infected the immortalized McCoy cell line, then used immunofluorescence and confocal microscopy to determine inclusion membrane properties. We found that C. abortus and C. caviae inclusions fuse greater than 80% of the time. Additionally, C. abortus inclusions appear able to undergo homotypic fusion and homotypic division. Multiple C. abortus inclusions were seen at low multiplicity of infection (MOI), but as MOI increased, so did the proportion of infected cells with multiple inclusions.
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  • Summer Undergraduate Research Experience (SURE)
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  • Pending Publication
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  • 2021-05-02 to 2023-06-03

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