Honors College Thesis
 

Development of Miniature Wire pH Sensors for Real-Time Monitoring of the Streptococcus Mutans Biofilm

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https://ir.library.oregonstate.edu/concern/honors_college_theses/br86bb85m

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  • The interface between a biofilm and the underlying substrate to which it is attached is challenging to study. Currently, available methods of studying this microenvironment have not been able to record data in real-time over a time period in the range of days. To address this challenge, we report the development of 300 μm diameter, potentiometric, and biofouling-resistant pH sensors, which were used to study the biofilm of Streptococcus mutans (S. mutans) over a period of three days. The sensor system employed had a combined drift of less than 0.01 pH unit per day, indicating that the system is usable on a time scale of days to weeks. The pH was found to drop from pH 7.2 at the introduction of S. mutans to 4.25 ± 0.54 (95% CI) after three days, which is acidic enough to cause significant tooth demineralization. This system could be used to study the concentrations of ions in a number of different biofilm-substrate microenvironments.
  • Key Words: Streptococcus Mutans, Biofilm, Potentiometric Sensor, Ion Selective Electrode
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