Honors College Thesis
 

Investigating the effects of harvest time and light exposure on cannabinoid potency in industrial hemp (Cannabis sativa) using UPLC-diode array detection

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https://ir.library.oregonstate.edu/concern/honors_college_theses/g732dh61f

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  • Five varieties of industrial hemp (Cannabis sativa) were grown in the field in Imperial Valley, CA, USA in order to determine whether light conditions or harvest times influence cannabinoid content. A total of 16 cannabinoids were investigated in this study: Delta-9-tetrahydrocannibinol (THC-d9), Delta-9-tetrahydrocannibinolic acid (THC-a), Cannabidiol (CBD), Cannabidiolic acid (CBD-a), Cannabigerol (CBG), Cannabigerolic acid (CBG-a), Cannabivarin (CBDV), Cannabivarinic acid (CBDV-a), Tetrahydrocannabivarin (THCV), Tetrahydrocannabivarinic acid (THCV-a), Cannabinol (CBN), Cannabinolic acid (CBN-a), Cannabichromene (CBC), Cannabichromene acid (CBC-a), Cannabicyclolic acid (CBL-a), and Delta-8-tetrahydrocannibinol (THC-d8). Cannabinoid potency in each sample was determined using UPLC-DAD analysis. Findings indicate that harvest time, but not light intensity, is a significant factor contributing to observed cannabinoid concentrations. Of the cannabinoids that showed a significant response to harvest time, 59% had the highest concentrations observed during the earliest harvest period (day 116 after seeding), while 41% displayed the highest concentrations during the two later harvesting periods (day 133 after seeding and day 152 after seeding). THC concentrations were shown to vary by as much as 0.24% dry weight, with highest THC concentrations consistently occurring during the earliest harvest period. The response of each specific cannabinoid varied considerably between the five varieties, indicating that plant genotype may be the most crucial factor in determining cannabinoid potency, as well as cannabinoidal response to external factors.
  • Key Words: Industrial hemp (Cannabis sativa), cannabinoid potency, light, harvest time, Ultra-Performance Liquid Chromatography (UPLC), Diode array detector (DAD)
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  • Duringer Lab - Environmental & Molecular Toxicology
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  • Funding for this project was provided by the Agricultural Research Foundation, the Continuing Undergraduate Research Support Group, and the Global Hemp Innovation Center.
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