Honors College Thesis
 

Predicting Protein-Protein Interactions in a Cnidarian Model System

Public Deposited

Downloadable Content

Download PDF
https://ir.library.oregonstate.edu/concern/honors_college_theses/gb19ff020

Descriptions

Attribute NameValues
Creator
Abstract
  • The symbiotic sea anemone Exaiptasia diaphana (Aiptasia) is a model organism used to better understand cnidarian-algal symbiosis and coral bleaching (dysbiosis). Many biological processes are hypothesized to be involved in coral dysbiosis, and the innate immunity is a key area of interest. Rab proteins are small proteins with GTPase domains, are involved in innate immunity responses including phagocytosis and endocytosis and have been previously observed to regulate symbiotic interactions in Aiptasia. Since molecular and biochemical assays can be resource intenseive, computational modeling and other in silico methods of biological processes are growing in popularity due to the benefits in reducing resource costs and can be a powerful tool for providing preliminary data. Here I investigate two Rab proteins from Aiptasia, apRab5 and apRab7 along with their hypothesized partner proteins OCRL and RILP, respectively, using Phyre2 homology modeling software and ClusPro protein docking to construct 3-D models and gather preliminary data for an in vitro Split-Luciferase Complementation Assay. Interactions of both protein partners gave promising results – showing that the luciferase fragments could reconstitute if encoded to the N-termini of Rab5 and OCRL and the C-termini of Rab7 and RILP in an in vitro follow up experiment.
Resource Type
Date Issued
Degree Level
Degree Name
Degree Field
Degree Grantor
Commencement Year
Advisor
Committee Member
Non-Academic Affiliation
Subject
Rights Statement
Publisher
Peer Reviewed
Language

Relationships

Parents:

This work has no parents.

In Collection:

Items

Thumbnail Title Date Uploaded Visibility Actions
file details GregoryDylan.pdf 2022-02-14 Public Download