Phenotypic Characterization of Exopolysaccharide Production in Lactococcus Public Deposited

http://ir.library.oregonstate.edu/concern/undergraduate_thesis_or_projects/x346d5951

Descriptions

Attribute NameValues
Creator
Abstract or Summary
  • Lactic acid bacteria (LAB) play a significant role in the production of several food products for human consumption. Humans exploit and maximize the various benefits from LAB characteristics, especially in foods such as cheese, yogurt and other fermented products. More recently, research discovered that certain LAB, such as Lactococcus species, produce unique exopolysaccharides (EPS). Prior research by E. Knoshaug, J. Ahlgren and J. Trempy with the bacterial strain Lactococcus lactis subsp. cremoris Ropy352 shows the production of a unique EPS in 10 w/w% nonfat milk, where it increased the milk’s viscosity to 24 Pa•s after 24 hours. Lyophilized samples of other Lactococcus species were retrieved from the Oregon State University Department of Microbiology Bacterial Strain Collection to investigate if other Lactococcus strains exhibit an EPS phenotypically similar to the EPS produced by L. Lactis subsp. cremoris Ropy352. Sixty Lactococcus strains were successfully cultured, of which 11 strains exhibited EPS phenotypically similar to the EPS produced by L. Lactis subsp. cremoris Ropy352 when inoculated in 2% milk. L. Lactis subsp. cremoris Ropy352 and the 11 Lactococcus strains produced the unique EPS in Lactaid milk, a lactose free milk, indicating lactose was not required in the production of this unique EPS. Further investigation was conducted to identify if any of the EPS producing Lactococcus strains contained the L. Lactis subsp. cremoris Ropy352 genes epsM and epsN required for the production of the unique EPS. Lactococcus strain #223 Lactococcus cremoris 18-1 has been characterized and found to contain the genes epsM and epsN.
Resource Type
Date Available
Date Issued
Non-Academic Affiliation
Keyword
Rights Statement
Language
Digitization Specifications
  • Poster made using Microsoft Powerpoint. PowerPoint file converted to .pdf using Microsoft PowerPoint for Mac 2011, Version 14.2.1.
Replaces
Additional Information
  • description.provenance : Approved for entry into archive by Sue Kunda(sue.kunda@oregonstate.edu) on 2012-05-29T16:52:47Z (GMT) No. of bitstreams: 1 revised Posterprest.pptx: 885215 bytes, checksum: 38ae35b69a1c365912a98cc4d3726032 (MD5)
  • description.provenance : Submitted by Roberto Garcia III (garcirob@onid.orst.edu) on 2012-05-29T04:34:18Z No. of bitstreams: 1 revised Posterprest.pptx: 885215 bytes, checksum: 38ae35b69a1c365912a98cc4d3726032 (MD5)
  • description.provenance : Made available in DSpace on 2012-05-29T16:52:47Z (GMT). No. of bitstreams: 1 revised Posterprest.pptx: 885215 bytes, checksum: 38ae35b69a1c365912a98cc4d3726032 (MD5)

Relationships

In Administrative Set:
Last modified: 07/21/2017 Default
Citations:

EndNote | Zotero | Mendeley

Items