Listeria monocytogenes contamination continues to pose challenges for the food industry and there is demand for effective methods of food preservation and protection that can also be considered clean label. A promising source of antilisterial compounds may be sourced from bacteria that produce novel byproducts. Ribosomally synthesized and post-translationally modified...
Corn oil (CO) and Sterculic foetida oil (SFO) fed rats were
injected with [9, 10-methylene-¹⁴C]sterculic acid. Less than 1%
of the label was expired as carbon dioxide. The majority of the
label was excreted in the urine as short-chain dicarboxylic acids with
an intact cyclopropane ring. The major metabolites for...
The objective of this study was to develop a decision making tool to determine economic feasibility of artisan cheese operations. A survey of current Oregon artisan cheese companies was used to gain knowledge of the fixed and variable costs associated with cheese production and business start-up. The data from this...
Porapak Q, Chromosorb 102, and Tenax GC, three porous
polymers commonly used as adsorbents in headspace analyses were
investigated. The retention times of various low-boiling compounds
relative to water were measured on the collection columns to determine
which compounds would be lost during the water removal step
employed after sampling...
The range of different nonvolatile constituents extracted from hops in highly hopped beers suggests that iso-alpha acids may not be the sole contributor to beers’ bitterness. Among brewers producing hop-forward beer styles there is a growing concern that the Bitterness Unit (BU) is no longer an accurate predictor of beer...
The traditional filter paper assay for saccharifying cellulase
originally described by M. Mandels et al (1976) has been modified to
make possible low activity determinations of Trichoderma
cellulases. The enzymatic activity appears to decline during a
prolonged incubation period if no precautions have been taken. By
means of adding bovine...
Whey is co-product of cheese production and is produced at 9:1 whey to cheese ratio. Whey is broadly classified into two types, sweet and acid whey. Large cheese manufacturers have developed whey processing techniques to convert sweet whey into value added products such as protein powders and emulsifiers. Acid whey...
The quality of wine is highly influenced by the weather.
Temperature, solar radiation and humidity influence the
formation and concentration of aroma-active compounds and
aroma-active precursors in the grapes. Pinot noir grapes require
a slow ripening, under cool temperatures, in order to achieve
their fullest flavor. The main objective of...
The free fatty acids of Cheddar cheese are important components
in the over-all flavor and aroma of the cheese. Although the
more volatile fatty acids probably contribute most, there is reason
to believe that the higher fatty acids may contribute, to some extent,
in imparting typical flavor characteristics. Before the...
In cross-cultural studies, respondents from specific cultures may have different product preferences and scale usage. Combining data from different cultures will result in departures from the basic assumptions of analysis of variance (ANOVA) and loss of power in testing capability of finding product and culture differences. However, the result of...
The problem in green pea grading system by using brine
flotation is loss of some less mature peas (generally
regarded as higher in quality and price) in the sinker
fraction (lower quality). Green peas were sampled from the
production line as follows: (1) blanched but ungraded peas,
(2) floater fraction,...
This work set out to examine the methodologies of dry hopping, compare different hop materials, and look at the extraction behavior of different types of hop compounds. This work consists of two discrete studies, where the first study informed the design of the second.
The first study measured the concentrations...
The DNA repair capabilities of rainbow trout (Salmo gairdneri)
were studied vising the method of autoradiography. Trout were fed a
semi-purified control diet containing 0 ppm, 50 ppm, or 300 ppm
cyclopropenoid fatty acids (CPFA) for 6-9 weeks. Liver slices were
prepared and exposed in vitro to a control treatment,...
This study was conducted to investigate the accumulation of dietary polychlorinated biphenyls (PCB's) by a Mt. Shasta strain of rainbow trout (Salmo gairdneri). A commercial mixture of PCB's (Aroclor 1254) was added to the diet at a level of 15 ppm on a dry weight basis. Accumulation of PCB's was...
The effects of ambient temperature storage on the chemical
and nutritional characteristics of phosphoric and sorbic acid-stabilized
fish autolysates were determined. Variations in sample
composition due to autolysis, deboning, and acidification were also
evaluated.
Autolysates of English sole (Parophrys vetulus), true cod
(Gadus macrocephalus), Dover sole (Microstomus pacificus), and
orange...
Acetaldehyde is known to be responsible for the green
or yogurt-like flavor defect of lactic cultures. This study was undertaken
to extend the knowledge of acetaldehyde production and utilization
by microorganisms normally found in mixed-strain butter cultures.
It is anticipated that the resulting information will contribute
to a more thorough...
Pacific shrimp (Pandalus jordani) was washed repeatedly and the
eluted bacteria were enumerated and identified. Selected isolates
were tested for their adhesive properties.
Washing reduced the microbial load by 3.84 to 42.04%. The bacteria
which most resisted wash-off were Staphylococcus and Pseudomonas
spp. The easiest to wash off was Flavobacterium...
The adsorption kinetics exhibited by selected charge mutants of T4 lysozyme at
silanized silica surfaces were monitored with in situ ellipsometry. Mutant lysozymes were
produced by substitution of lysine (Lys) with glutamic acid (Glu). Each substitution
resulted in a decrease in the net charge of the protein by 2 units....
Cyclopropenoid fatty acids (CPFA) are natural components of
cottonseed oil, a major food oil in the United States. The ability of
CPFA to cause abnormal biochemical and physiological effects when
fed to laboratory and farm animals has prompted an investigation of
their effects on mice.
Between 0.05 and 0.55% CPFA...
The major portion of Pacific whiting (PW) is commercialized in the form of frozen surimi. Alternative products for PW were investigated focusing on fresh surimi and texturized meat from PW mince. Fresh surimi is made without additives and kept refrigerated instead of frozen. Texturized meat is a meat-like product made...
The aroma of Tuber gibbosum, a native Oregon truffle, was
characterized using two distinct techniques. Volatile aroma
constituents were identified by chemical analysis, and sensory
characteristics were determined by examining response of the
general populace to the truffle aroma.
Truffle samples from four maturity ranges, as determined
by microscopic examination...
Blackberries, genus Rubus, are an important Oregon agricultural commodity. In their fresh and processed forms, they offer many health benefits and may be able to help Americans better achieve fruit consumption recommendations because of convenience and pleasant sensory qualities. However, the susceptibility of blackberry products to contamination with bacterial pathogens...
The composition and some qualitative aspects of ground beef
offered for sale in Corvallis, Oregon were studied. Samples were
purchased from six retail markets twice weekly throughout a six
week test period. Three stores were independently owned and operated
and three were chain stores.
Differences in moisture, fat and protein...
Pasteurization is an effective method of reducing risks associated with foodborne pathogens in raw milk. Cheese made from raw milk has a higher potential to harbor harmful bacterial pathogens. However, some cheesemakers prefer to use unpasteurized milk to preserve the natural microflora present in the raw milk and to prevent...
Chilling (2-4°C) (10 min) in a solution containing 10% condensed
phosphate, 5% potassium sorbate, and 0.9% citric acid (pH 6.7)
stabilized round shrimp to microbial spoilage. This treatment procedure
allowed refrigerated storage of round shrimp eliminating the
need for ice and the accompanying deteriorative effects of water
absorption and protein...
A simple process to obtain onion oil by direct solvent extraction
was proposed. Several solvents commonly utilized in the food
industry were tested for their selectivity in the extraction of the
oil of onion. Diethyl ether and methylene chloride appeared to be
the most suitable solvents for the extraction operation....
Raw milk microbiome is important to food safety and security. However, understanding the complexities of how the dairy farm environment may affect the microbiome is difficult. Since there are a variety of operating parameters for dairy farmers to use on their farms, each farming environment is unique. What makes the...
Radish anthocyanin extract has potential as a natural colorant because of
its pigment stability and attractive red hue. Presence of undesirable aroma
compounds could limit its applications in foods. The pungent principle of
radish, 4-methylthio-3-butenyl isothiocyanate (MTBI), is produced
enzymatically upon cell injury from its glucosinolate precursor, 4-methylthio-3-butenyl glucosinolate (MTBG),...
Kappa-casein glycomacropeptide (CMP), a 64-amino-acid peptide, is released from kappa-casein after rennet treatment and is one of the major peptides in whey protein isolate (WPI). CMP has anti-inflammatory and antibacterial activities. CMP has two major amino acid sequences with different modifications including glycosylation, phosphorylation and oxidation. However, no previous work...
Proanthocyanidins (PACs) containing A-type linkages, like those found in cranberry, have been implicated as beneficial phenolic compounds in human health, but much research is still required to better understand their activity fully. One major impediment to progress has been the existence of effective analytical methods for A-type PAC quantification. This...
The German hop Hallertauer, hallertauer is highly
valued because of its "noble aroma", but it has a very low
yield when cultivated in the U.S. Two new crosses of
Hallertauer, U.S.D.A. 21455 and 21459, have high yields
and promising aroma characteristics.
In order to predict sensory properties of beers as...
The literature available on the silica content of herbaceous biomass, and its relative mobility in leaching/extraction systems, includes studies that have employed several different methods for silica quantification. This makes comparison of the data difficult. The objective of this thesis research was to determine the quantitative relationships between the measured...
Anthocyanin degradation in freeze-dried strawberry puree was studied under various relative humidity conditions at 37°C. Moisture was found to be the determining factor in the rate of pigment degradation. Storage at relative humidities below 11% provided good pigment retention in this low-moisture product. Enzymatic degradation of the anthocyanin pigment did...
The pigments of blackberry fruit were investigated by analytical and preparative high performance liquid chromatography (HPLC) to characterize anthocyanin patterns in commercially important blackberry (Rubus eubatus) cultivars and selections. Five anthocyanin pigments were detected in Evergreen blackberry and they were identified as cyanidin-3-glucoside, cyanidin-3-rutinoside, pentosecyanidin derivative and two acylated cyanidin...
Seven red raspberry (Rubus Ideaus L.) samples from Oregon, Washington, British Columbia and New Zealand were processed into juice. These, along with three juice samples supplied from West Germany were analyzed by high performance liquid chromatography, (HPLC), for nonvolatile acid, sugar, anthocyanin and anthocyanidin composition. In
order to quantitate the...
Ample research has demonstrated the largely untapped potential of bioactive peptides (BAPs) for treating systematic chronic inflammation (SCI). Protein hydrolysates are the mixture of peptides resulting from the hydrolytic breakdown of a protein. Numerous hydrolysates contain BAPs and exert beneficial effects in both in vitro and in vivo models. In...
Seaweeds have gained tremendous recognition as a highly nutritious and sustainable crop. The diverse components of seaweeds are known to exhibit a wide range of bioactivities, including anti-inflammatory, anti-diabetic, and cardiovascular health benefits. One such promising seaweed is Pacific dulse (Devaleraea mollis). While it holds great cultivating potential on the...
Varying concentrations of the food preservatives nisin and lysozyme were adsorbed onto glass surfaces chemically modified to exhibit different degrees of hydrophobicity. The antimicrobial activity of the adsorbed preservatives was evaluated by documenting the ability of Listeria monocytogenes to adhere and grow on the glass surfaces. A bioluminescence protocol was...
There have been several epidemiological studies linking alcohol
consumption to a decreased risk of contracting illness from foodborne
bacterial contamination. To study this phenomenon, we examined the
survival of Escherichia coli 0157:H7 and Salmonella typhimurium in grape
juice and wine and then designed a model stomach to investigate the effect...
It has been suggested that cellular damage from oxygen radicals is one of the processes leading to cardio-vascular disease and cancer. Natural antioxidants prevent uncontrolled oxidative reactions by decreasing molecular oxygen levels, scavenging chain-initiating and chain-propagating free radicals, chelating metals, or decomposing peroxides. Beer is rich in antioxidants, derived both...
Surimi is a seafood product which is used to manufacture restructured products
such as artificial crab and lobster. Surimi is produced from fish fillets by washing to
remove sarcoplasmic proteins and increase the concentration of myofibrillar proteins, and
mixing with cryoprotectants. A valuable attribute of surimi is its ability to...
The distribution of total phenolics, antioxidant capacity, monomeric anthocyanin, and ascorbic acid contents in the peel and flesh of Red Delicious, Granny Smith, and Fuji apples during a six-month storage period were determined. In addition, the effect of 1-MCP (1-Methyl cyclopropene) on these parameters of the edible portion of apples...
Vibrio parahaemolyticus is the leading cause of foodborne illnesses associated with seafood consumption. Consuming raw or undercooked seafood contaminated with V. parahaemolyticus can result in development of acute gastroenteritis with symptoms of nausea, vomiting, abdominal cramping and watery diarrhea within 24 hours of infection. Illness is usually self-limited and lasts...
Fish sauce is a fermented condiment made primarily from anchovies and is popularly used in Asian countries as it provides unique salty and umami flavor. Its consumption is no longer limited to Southeast Asian countries, but extended to Europe, United States and Canada as a condiment in their food. Fish...
Pacific oysters (Crassostrea gigas), Alaska pink salmon (Oncorynchus gorbuscha) roe and chum salmon (Oncorynchus keta) fillets were treated with aqueous ozone in both pilot plant and commercial settings to determine its effect on shelf-life and microbial changes. The microbial quality was analyzed by conducting pyschrotrophic and coliform plate counts on...
Vibrio parahaemolyticus is a human pathogen which is prevalent in marine environment. Consumption of raw or undercooked seafood contaminated with V. parahaemolyticus can cause foodborne illness. This study investigated the application of probiotics in depuration for reducing V. parahaemolyticus in raw Pacific oysters (Crassostrea gigas) and the utilization of green...
The fermentation of honey-based musts has been a feature of civilization for millennia. Typically comprised of 75-90% (w/w) simple (i.e. fermentable) sugars, the glucose and fructose present are readily fermented by either pitched or adventitious micro-organisms. Nevertheless, despite its venerable heritage, honey-based fermentations are prone to inconsistency, and it is...