This study was undertaken to compile a comprehensive
review of the literature and to gain a better understanding
of the host-parasite relationship in corynebacterial kidney
disease of salmonid fishes. An indirect fluorescent antibody
technique was developed which allowed differentiation
of intra- and extracellular bacteria. This technique was
used in combination...
This project was initiated in an attempt to control furunculosis
(Aeromonas salmonicida) in a production hatchery rearing coho salmon.
An oral vaccine was prepared and administered to 520,000
juvenile coho salmon (Oncorhynchus kisutch) at the Siletz River Salmon
Hatchery prior to the onset of a predictable naturally occurring
epizootic of...
Experiments were designed to more precisely evaluate the effects of water temperature on the progress of bacterial kidney disease ( BKD) in three species of salmonid fishes. Infections were produced by intraperitoneal injection of suspensions of the causative organism into fish held at seven temperatures ranging from 3.9 to 20.5°C....
The antigens of eight isolates of Renibacterium
salmoninarum were compared serologically using
immunoelectrophoretic techniques. Seven common antigens
were identified by their electrophoretic mobilities in each
antigenic profile. One of these common antigens, designated
Fr 21-30, was partially purified by ammonium sulfate
precipitation and gel filtration. Cross adsorption analysis
showed Fr...
The detection and antigenic nature of the causative Corynebacterium
of bacterial kidney disease and chemotherapy of this disease
in juvenile coho salmon (Oncorhynchus kisutch) were examined.
Each of 207 yearling coho salmon collected from a population
undergoing a severe epizootic of bacterial kidney disease were examined
for the presence of...
The purpose of this study was to find what effect temperature
and immunization has on the phagocytic activity of fish leukocytes
against certain disease causing bacteria. Also proposed was the
isolation and characterization of a leukocytolytic factor produced by
one of these bacteria, Aeromonas salmonicida.
Investigations revealed that temperature had...
The purpose of this project was to study the possibility of controlling
furunculosis in salmonid fish using immunological procedures.
Antiserum with an agglutinating antibody titer of 1:20,480
against Aeromonas salmonicida was produced in a horse. This
hyperimmune serum was used to passively immunize coho salmon,
Oncorhynchus kitsutch, (jacks). However, this...
An additional layer, exterior to the outer membrane cellwall
layer, was found on Aeromonas salmonicida cells from aggregating
strains. Nonaggregating strains were found to lack this layer.
The nonaggregating strains were all avirulent (LD₅₀
greater than
1 X 10
⁸ CFU/fish) while the majority of the aggregating strains
were virulent...