Mycoplasma haemolamae is associated with mild to marked anemia in stressed, immune-suppressed, and debilitated animals, and may be found in low numbers in healthy animals. The continued presence of the organism, detectable by polymerase chain reaction (PCR)-based assay, may be associated with an underlying problem, such as stress or immune-suppression...
Sweet onions are valued for their unique flavor and praised for their health and dietary character. Onion sweetness is perceived as the degree of pungency decreases. Pungency can be quantitatively determined in onions by measuring enzymatically produced pyruvic acid. The onion industry quantifies a sweet onion as one having a...
Perfluorinated chemicals have been widely used in industry for their superior surfactant qualities. Because of their widespread usage and resistance to photolytic and biodegradation mechanisms, as well as their ability to undergo long-range atmospheric transport, these compounds have become ubiquitous in the world-wide water supplies. Of particular interest are perfluorooctanoic...
Tumor necrosis factor alpha (TNF α) and interferon gamma (IFN γ) were initially recognized as inflammatory mediators produced by immune cells in response to infectious agents or foreign material. (1,5) However, over the past decade much research in mammalian species has indicated that these proteins also play key roles in...
Antibiotic resistance is an increasing problem in many species of bacteria today, with pathogens an important focus. Fecal contamination of shellfish is already a concern due to potential pathogens. This report examines the possibility of resistance gene transfer between microbes, due to fecal bacteria, within the oyster. In this study,...
Agricultural areas can be inhospitable to the attraction and maintenance of beneficial insects like natural enemies and pollinators and this can result in a decrease in the ecosystem services of pollination and pest control that these insects supply. If habitat can be created and maintained on-farm, an increase in the...
Pesticides are ubiquitous, with more than one billion tons of pesticide products used in the United States annually. These compounds are characterized by their toxic effects to the target organism; however, pesticides are also well known for their deleterious effects to non-target species. Because pesticides have ecological and human health...
Cancer is class of diseases that has great diversity and unfortunately is one of the leading causes of death in the United States. Chemotherapy remains an important cancer treatment modality, and superior chemotherapeutic drugs are constantly being sought. Gemcitabine is an anti-tumor chemotherapeutic drug and it is the first line...
Chitosan based hemorrhage control dressings provide effective external hemorrhage control while having few side effects. Known residual contaminants present in chitosan formulations include endotoxins and proteins known to stimulate gene expression in the liver. This study investigated gene expression in the liver using rat bio-compatibility models to gain knowledge regarding...
Renibacterium salmoninarum is the causative agent of bacterial kidney disease in both
wild and farmed salmonid species worldwide. The genome of this pathogen has
significant synteny to the ubiquitous, soil-dwelling Arthrobacter spp. though it is 1.9 Mb
smaller, suggesting that reductive evolution has occurred. Recently, our group finished
sequencing and...
The ability of soils to store large quantities of carbon (C) makes them an important factor in the global C cycle. Small changes within soil C can lead to large changes in atmospheric C. Land management practices can influence the amount of C that is stored within the soil and...
The goal of this project was to develop and test non-herbicidal techniques that remove nonnative plant or weed seeds from the top 2 inches of the soil. The Nature Conservancy (TNC) is currently restoring prairies in the Willamette Valley. Some areas of the Pacific Northwest have already seen loss of...
Methane hydrates and biohydrogen are two disparate subjects that are of interest in the growing field of alternative energy research. However, before commercial use becomes a viable option, each has challenges that need to be overcome. Methane hydrates require large investments of time and capital to locate. In this thesis,...
Organophosphate insecticides may produce sublethal, adverse effects on salmonid
growth, reproduction, maturation, swimming, and feeding. Chlorpyrifos, an
organophosphate insecticide, was detected by the Oregon Department of Environmental
Quality in the Hood River and tributaries during March 1999 at levels exceeding state
water quality standards in the range of 0.011-0.482 μg/L....
Port-Orford-cedar (POC) (Chamaecyparis lawsoniana (A. Murl.) Parl) is an
important tree species native the coastal ranges of southern Oregon and northern
California. The tree is a false-cedar in the cypress (Cupressaceae) family and is used for
timber and planted as an ornamental. The tree in most of its native range...
Polycyclic aromatic hydrocarbons (PAH) are common pollutants of aquatic systems in urban environments. Due to their lipophilicity, persistence, toxicity and carcinogenicity, they are chemicals of considerable concern. The Portland Harbor includes a large industrial section with numerous current and historic sources for PAH contamination. Runoff from a large metropolitan area...
Deforestation, intensive agriculture and chemical use all can cause long-lastingn negative impacts on soil health. With all the vital functions that soils perform and the potential for further soil degradation, it is important that standards of soil quality or soil health be implemented. Soil aggregation is considered to be a...
Five new anti-zebrafish cytochrome P450 (CYP) antipeptide polyclonal antibodies were tested for tissue specificity using immunohistochemistry. The antibodies are for the zebrafish CYPs IA, 2K6, 2K7, 3A65 and 3C1. Three of these antibodies were also compared to existing antibodies made against CYP isozymes in rainbow trout (Oncorhynchus mykiss). Individual staining...
The cabbage maggot, Delia radicum (L.) (Diptera: Anthomyiidae) is a serious
pest of cruciferous crops (i.e. broccoli, cabbage, turnip, rutabaga) in North America and
Europe (Coaker and Finch, 1971; Whistlecraft et al., 1985). The adult flies lay eggs
around the base and in cracks at the soil surface around wild...
Northwest cherry cultivars including Prunus avium 'Bing', Prunus avium 'Rainier', Prunus avium 'Royal Anne' and Prunus cerasus 'Montmorency' were separated into epidermal tissue, flesh and pit and cryogenically milled with liquid nitrogen. These samples as well as whole samples from each variety were extracted with acetone followed by a chloroform...
Little is known about Frankia, a gram-positive, nitrogen-fixing bacterium, because of the difficulty associated with isolating pure cultures. This nitrogen-fixing symbiosis involves Frankia and the roots of actinorhizal host plants belonging to eight plant families and 25 genera. These plants are important pioneer species traditionally found in nitrogen-poor soils and....
Little is known about upland agricultural grassland soil invertebrate composition and its contribution to ecosystem functioning. Soil-dwelling organisms play a central role in soil formation, plant nutrition, and are significant food contributors for organisms in several trophic levels. Information is needed about mesofauna seasonal population and composition trends throughout the...
Plasminogen activators (PAs) have been implicated in: ovulation, oocyte maturation, fertilization, implantation, and many other reproductive activities. Three pathways have been implicated in the activation of the PA regulatory system. These include the conventional PKC and cAMP-dependent signaling pathways, and also an independent pathway thought to be regulated by protein...
Saturated hydraulid conductivity (Ks) is a central soil physical property in determining water movement and contaminant transport through soils; hence modeling this system requires an accurate determination of K. Decimeter-scale KS measurements employing cores or infiltrometers are used frequently, but field-scale KS values are required to encompass the full range...
A set of flour samples with protein content ranging from 9.7 - 13.3 % was used for this study to evaluate the effectiveness of HMW-GS, SEHPLC and mixograph characteristics as screening tools for noodle texture. The sample set had a large variation in HMW-GS composition with Payne score ranging from...
An organisms' genotype may not be reflected in its phenotype. For example, epistasis - the interaction of non-allelic genes can cause deviations from expected phenotypic ratios. The epistatic interaction between the hooded (Kap) and short-awned (lks2) loci in barley (Hordeum vulgare) precludes the localization of the Kap locus, based on...
Horticultural and nursery crops are the leading agricultural commodity in Oregon. Among currently grown nursery plants, Verbena x hybrida is a staple ornamental. It is grown and shipped worldwide, thus requiring a high level of certainty concerning the plants’ health and potential viability. V. x hybrida cultivars have been infected...
Cucurbits are grown in production agriculture in Oregon as well as in smallholder agriculture in tropical South America. Experimental research was performed at the OSU Research Farm in 2001 with direct-seeded cucurbits, and literature reviews were conducted to develop a characterization of smallholder farming in tropical South America. The two...
Gaps in the forest canopy allow more light to reach the understory. Since
light in the understory is heterogeneous, understanding the physiological
processes in gaps and non-gaps will improve estimates of carbon balance. This
research outlines an approach for defining carbon balance for Salal (Gaultheria
shallon Pursh.), a dominant understory...
Soil CO2 efflux is a major contributor of CO2 to the atmosphere. The rate that CO2 is respired out of the soil is called the soil flux (Fs). Many variables alter Fs. These variables differ drastically in the variety of ecosystems found on the earth. It is important to understand...
The ionotropic glutamate receptors are ligand-gated ion channels that mediate the majority of excitatory neurotransmission in the brain. The three classes of ionotropic glutamate receptors are alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), N-methyl-D-aspartate (NMDA), and kainite. These three classes have unique subunits. NMDA receptors play important roles in development, forms of synaptic plasticity,...
Chlamydiae are obligate intracellular bacterial pathogens with a unique biphasic life cycle that consists of an infectious but non-dividing form, called elementary bodies (EB), and a noninfectious but metabolically active form, called reticulate bodies (RB). Chlamydia has an unusual process of cell division of which still remains uncharacterized subject matter;...
The acorn woodpecker (Melanerpes formicivorus) is among the most common primary cavity nesters of the Oregon white oak (Quercus garryana) woodlands. Understanding their selection of granary sites is important in evaluating habitat quality and management actions. I hypothesized that granary locations would be consistent with the optimal foraging theory. Site...
COUP-TF interacting protein 1 (CTIP1) is a novel C2H2 zinc finger protein that mediates transcriptional repression of ARP1, a member of the COUP-TF subfamily of orphan nuclear receptors [1]. Here we show that CTIP1 interacts with heterochromatin-associated protein 1 (HP1) in vitro and in cells. This
interaction suggests the existence...
Grain protein concentration of wheat is influenced by numerous factors. When determining a wheat variety's ability to produce protein, the plant's ability to uptake and translocate nitrogen is critical. A greenhouse study was conducted to assess differences in nitrogen use among different wheat varieties. The objective was to determine if...
Various vineyard practices influence the quality of a wine. In 1999, three levels of nitrogen (N), two levels of tillage and two levels of irrigation were applied to Pinot noir grape cultivars at Benton Lane Vineyards in the Southern Willamette Valley. N treatments were zero, soil and foliar applications. Every...
Crystallizing a virus can be difficult and not always successful. Forty-eight salts, buffers, and precipitating agents were used in the hanging drop preparations in attempts to crystallize the San Miguel sea lion virus type 5 (SMSV-5). The first attempt resulted in 22 of the 48 solutions forming a precipitate. The...
Indole-3-carbinol (13C), a metabolite of glucobrassicin, is found in cruciferous vegetables. It is able to act as an inhibitor or promoter of carcinogenesis depending on the species, the carcinogen and when it is given in relation to the carcinogen. This is probably due to the fact that it reacts in...
The timber and Christmas tree industries are important components of the Pacific Northwest economy. A goal of both industries is to produce high quality trees with high growth rates. Accomplishing this requires increased genetic quality of the trees grown. Tissue culture provides the most promise for propagating these superior genotypes....
Age-related decline in the capacity of endogenous antioxidant systems
complicates pharmaceutical use by the elderly by potentially increasing the toxicity of
some compounds. Of particular interest is chemical detoxification via glutathione (GSH)
and attendant GSH-requiring detoxification enzymes, e.g. GSH peroxidases (GPX) and
GSH S-transferase (GST). These enzymes have been shown...
Kincaid's lupine (Lupinus sulphureus ssp. kincaidii [Smith] Phillips) is a federally listed Threatened plant of Willamette Valley prairies. Both low reproduction, due to seed coat dormancy, and competition from invasive plants may threaten the longterm viability of this species. In separate studies, I investigated seed germination and an invasive species...
An analytical method was adapted to extract semi-volatile organic compounds (SOCs) from conifer needles. Needles from Abies concolor
(white fir) and Pinus contortas (lodgepole pine) from Sequoia National Park (SNP) were collected during the summer of 2003, and analyzed for SOCs. The goal of this research was to determine differences...
Previous research suggests a link between litter size in ewes and differential excretion of fecal hormones. An accurate test for fetal number would be useful to sheep producers and a fecal hormone response could be utilized in such a test. Fifteen previously categorized fecal hormones were analyzed within fecal samples...
The nursery industry plays a critical role in Oregon's economy. An increase in wood-boring insect, or borer, activity has impacted the industry. The goal of this research is to determine key borer species in the nursery industry, flight times so targeted spray schedules can be established, and assess grower knowledge...
A genetic selection study to determine the effects on egg hatchability and subsequent chick performance of Japanese quail eggs incubated at a 38.9° C (102° F) dry bulb temperature (Selected, S) when compared to 37.8 C (100°F) (Control, C) was conducted for four consecutive generations. Eggs from a randomly mated...
Raspberry bushy dwarf virus (RBDV) infects red raspberry and black raspberry worldwide. Infection causes reduced vigor and drupelet abortion leading to crumbly fruit and reduced yields. While this crumbly fruit can still be used in juice, jam, and preserves, it cannot be used for the more valuable fresh and individually...
The diageotropica (dgt) mutation has been proposed to affect either auxin
perception or responsiveness in tomato plants. Auxins and cytokinins are plant
growth regulators known to interact with each other via a variety of mechanisms.
Here, we report the interactions of auxins and cytokinins in VFN8 wild-type and
dgt mutant...
