Index Catalog // ScholarsArchive@OSU

Internal pH control starter media: use in culture preservation and for multiple strain growth

Creator:: Shigeno, Debra S.
Abstract:: Several bulk starter media, consisting of two experimental blends and four commercial preparations, were examined for each medium's ability to grow, preserve, and/or recover lactic streptococcal starter cultures. Nonfat milk was used as a control. Results showed that cultures which had the greatest survival rate in frozen storage had been...
Resource Type:: Masters Thesis
Full Text:: Privacy Dr. William E. Sandine Several bulk starter media, consisting of two experimental blends and

Ecological study of aquatic myxobacteria in a woodland stream

Creator:: Carlson, René Virginia
Abstract:: Most studies of bacteria in water have concerned public health; little attention has been given to organisms which are indigenous to the aquatic environment. Myxobacteria are known to occur in soil, in the marine environment, and several have been studied in relation to diseases of salmonid fishes. However, little information...
Resource Type:: Masters Thesis
Full Text:: A WOODLAND STREAM Abstract approved: Redacted for Privacy kobert E. Pacha Most studies of

Characterization of thermophilic rod and coccus starter strains used in mozzarella cheese manufacture

Creator:: Faessler, Patrick Charles
Abstract:: The present investigation was undertaken to characterize a number of strains of Lactobacillus bulgaricus and Streptococcus thermophilus intended for use by a commercial starter supply company. Thorough characterization of each culture was required in order to combine compatible strains so that their usefulness in Mozzarella cheese manufacture would be maximized....
Resource Type:: Masters Thesis
Full Text:: Redacted for Privacy Abstract approved: Dr. William E. Sandine The present investigation was

Characterizing Mycobacterium avium subspecies hominissuis (MAH) Microaggregate Induction of Innate Immunity

Creator:: Keefe, Bailey F.
Abstract:: Bacterial aggregation is a strategy employed by many pathogens to establish infection. Mycobacterium avium subsp. hominissuis (MAH) undergoes a phenotypic change, microaggregation, when exposed to the respiratory epithelium. This aggregation is an important step in the pathogenesis of the infection, laying the foundation for biofilm formation. We therefore compared how...
Resource Type:: Masters Thesis
Full Text:: . Emerg Infect Dis 17:1760–1761. 9. Winthrop KL, McNelley E, Kendall B, Marshall-Olson A, Morris C

Effects of propionibacterial metabolites on spoilage and pathogenic bacteria in dairy products

Creator:: Teo, Alex Yeow-Lim
Resource Type:: Masters Thesis
Full Text:: Metabolites On Spoilage and Pathogenic Bacteria in Dairy Products Abstract approved by: William E. Sandine

Lactic streptococci: growth characteristics and plasmid profiles of drug- and bacteriophage-insensitive mutants

Creator:: Wulf, John J.
Abstract:: Fast acid-producing streptomycin-resistant mutants for several strains of lactic streptococci currently used in commercial Cheddar cheese manufacture were isolated. Their isolation involved use of a recently developed differential plating medium that allows identification of fast acid-producing colonies. Both parents and mutants were characterized with regard to growth, acid production, proteolytic...
Resource Type:: Masters Thesis
Full Text:: : Redacted for Privacy William E. Sandine Fast acid-producing streptomycin-resistant mutants for several

Studies on the inhibition of spoilage organisms in Cottage cheese by lactic streptococcus organisms

Creator:: Hauser, Bruce Allen
Abstract:: A report in the literature suggesting that the use of cottage cheese dressing cultured with S. diacetilactis would afford enhanced flavor and shelf life in the finished product prompted this study to examine reasons for its effectiveness. The method used involved addition of a culture of S. diacetilactis 18-16 to...
Resource Type:: Masters Thesis
Full Text:: Redacted for Privacy Abstract approved Dr. W. E. Sandine A report in the literature suggesting that

A taxonomic study on myxobacteria isolated from fish

Creator:: Porter, Sook Hee
Abstract:: While the genus Cytophaga has been defined and classified on a morphological basis, knowledge of fresh water members of this group of myxobacteria is scanty. In the present study cultural and morphological characteristics were investigated in an attempt to classify a group of fresh water cytophaga. Microscopic examination revealed all...
Resource Type:: Masters Thesis
Full Text:: Redacted for privacy Abstract approved: Dr. R. E. Pacha While the genus Cytophaga has been defined and

Isolation and characterization of a bacteriophage for Cytophaga succinicans (RL-8)

Creator:: Rust, Patricia Joy
Resource Type:: Masters Thesis
Full Text:: : fIlDr. R. E. Pacha Although bacteriophages have been recognized for a number of years, little

Growth of lactococci relative to antibiotic and quaternary ammonium compounds

Creator:: Valladao, Marilin
Abstract:: The work presented in this thesis is concerned with the effect of several antibiotics and quaternary ammonium sanitizers upon growth of lactic acid bacteria. Section I reports the purification of beta-lactamase from Lactococcus cremoris PR-108, by ion exchange chromatography, using the chromogenic substrate pyridine-2-azo-p-dimethylaniline (PADAC) as the enzymatic indicator. Section...
Resource Type:: Masters Thesis
Full Text:: AMMONIUM CrOUNDS Redacted for PrivacyAbstract approved: William E. Sandine The work presented in this

Evaluation of peptide phosphorodiamidate morpholino oligomers as a novel antibiotic therapy

Creator:: Kaller, Margo
Abstract:: Peptide-phosphorodiamidate morpholino oligomers (PPMOs) are synthetic DNA mimics that bind and silence gene targets. Through designing PPMOs that silence essential or resistance genes in pathogens, these antisense oligomers could be utilized as novel antimicrobials. Towards this end, my thesis employed minimum inhibitory concentration assays (MICs) to identify PPMOs capable of...
Resource Type:: Masters Thesis
Full Text:: inhibitory concentration assays (MICs) to identify PPMOs capable of inhibiting in vitro growth of E. coli

Molecular characterization of crustacean parasite Nadelspora canceri

Creator:: Amogan, Harold
Abstract:: Investigations into the phylogeny, genome size, and karyotype of microsporidian Nadeispora canceri were initiated to further characterize the organism. Isolates of N. canceri spores were obtained from both Dungeness (Cancer magisrer) and red rock crabs (Cancer productus). Analysis of the ssu rDNA sequence from spore isolates of the two crab...
Resource Type:: Masters Thesis
Full Text:: would also like to thank Dr. Robert Olson for his help in specimen collection and the OSU Central

Analysis of Bacteroidales 16S rRNA gene sequences from a geographically isolated human population

Creator:: Petersen, Carolyn A.
Abstract:: Standard methods of measuring fecal pollution in water do not distinguish between human and non-human sources. Molecular technology enabled the development of host-specific markers that distinguish fecal sources. Human specific PCR primers, HF183F and HF134F, were designed based on phylogenetic analyses of partial 16S rRNA gene sequences from the Bacteroidales...
Resource Type:: Masters Thesis
Full Text:: 13 2.6.1. Coliform-specific PCR... 14 2.6.2. E. coli toxin gene-specific PCR

Bacterial 16S ribosomal DNA analysis of pyrrolizidine alkaloid detoxifying enrichments from the ovine rumen

Creator:: Gray, Diane R.
Abstract:: Bacterial cultures enriched from sheep rumen fluid have demonstrated the ability to detoxify pyrrolizidine alkaloids (seneciphylline and jacobine) in tansy ragwort (Senecio jacobaea). The microbes are difficult to isolate using classical anaerobic techniques, therefore, microbes from two different enrichment cultures demonstrating similar degradation activity were identified using their 16S ribosomal...
Resource Type:: Masters Thesis
Full Text:: chart of methods 42 2.3 Components of TYM and E media used to grow enrichment cultures 44 2.4

Evolution of a streamlined cooperator in Pseudomonas aeruginosa quorum sensing

Creator:: Huie, Jessica L.
Abstract:: Social behavior leading to the production of common goods is prone to exploitation. One such behavior in Pseudomonas aeruginosa is quorum sensing (QS), by which the bacteria produce signals to regulate extracellular common goods. Exploitation comes in the form of cheaters which have a mutation in the central quorum sensing...
Resource Type:: Masters Thesis
Full Text:: pJHlasR5, pJHpsdR1, and pJHabcB1. The plasmids were transformed into E. coli DH5α Max Efficiency cells

DNA vaccines encoding the glycoprotein genes of spring viremia of carp virus, snakehead rhabdovirus, or infectious hematopoietic necrosis virus induce protective immunity in rainbow trout (Oncorhynchus mykiss) against an infectious hematopoietic necrosis virus lethal challenge

Creator:: Drennan, John D.
Abstract:: Recent advances in DNA vaccine technology has brought about a promising strategy for the control of viruses that contain surface membrane glycoproteins. This type of vaccine involves the intramuscular injection of a bacterial plasmid containing a gene encoding a viral protein. The strategy uses eukaryotic processing of the protein as...
Resource Type:: Masters Thesis
Full Text:: isotype switching. (Lobb & Olson, 1988; Killie et al., 1991) The absence of affinity maturation is

Studies on cellulase production from annual ryegrass straw by Trichoderma reesei

Creator:: Orberg, Paulo K.
Resource Type:: Masters Thesis
Full Text:: Dr. R. R. Becker and Dr. W. E. Sandine for kindly revising the manuscript, and to Dr. D. L. Willis

Establishment and characterization of three new cell lines from grass carp (Ctenopharyngodon idellus)

Creator:: Lu, Yuanan
Resource Type:: Masters Thesis
Full Text:: suggestions, and encouragement. His humor, criticism, and friendship were appreciated. To Dr. Robert Olson

Comparison of Renibacterium salmoninarum by antigenic analysis, antimicrobial assay and growth

Creator:: Getchell, Rodman Guy
Abstract:: The antigens of eight isolates of Renibacterium salmoninarum were compared serologically using immunoelectrophoretic techniques. Seven common antigens were identified by their electrophoretic mobilities in each antigenic profile. One of these common antigens, designated Fr 21-30, was partially purified by ammonium sulfate precipitation and gel filtration. Cross adsorption analysis showed Fr...
Resource Type:: Masters Thesis
Full Text:: to Dr. J. E. Sanders for reviewing this manuscript, Dr. J. Leong for her assistance, Rich Holt for

Suppression of cellular immune responses by Candida albicans

Creator:: Rivas Espinoza, Victor G.
Abstract:: Experimental infection of mice with Candida albicans leads to significant suppression of cell mediated immune responses. We have characterized the nature of this candida-induced suppression with formalin killed Candida albicans (FKCA) in the yeast and mycelia' phase. Mice (BALB/c or C57B1/6J) treated with FKCA exhibited a reduction in the T-cell...
Resource Type:: Masters Thesis
Full Text:: manuscript. The Universidad Veracruzana and Asociacion Nacional de Universidades e Institutos de Enzeflanza

Immune responses of juvenile chinook salmon (Oncorhynchus tshawytscha) to p,p-̓DDE and tributyltin

Creator:: Misumi, Ichiro
Abstract:: In this thesis, we examined the effects of the exposures to anthropogenic pollutants on the fish, primarily juvenile chinook salmon, immune system using newly and recently developed immune assays. In addition, we developed a new assay for measuring immunocompetence of fish. In the first chapter, the Alamar Blue assay was...
Resource Type:: Masters Thesis
Full Text:: and Kruskal-Wallace test). 2.6 2.4 a) 0 2.O 1.8 C', E 1.6 1.4 1.2 1.0 2.4 2.2 >< a) -o

Hematology and histopathology of Coho salmon (Oncorhynchus kisutch) infected with Flexibacter psychrophilus

Creator:: Kanchanakhan, Somkiat
Resource Type:: Masters Thesis
Full Text:: ProfessoKand Chairm the Department of Microbiology in charge of major Redacted for Privacy Dean of the Grad e

Analysis of segregants of F'gal heterogenotes in Escherichia coli

Creator:: Kurahara, Carole Mahaney
Abstract:: The purpose of this study was to isolate and characterize any segregants of the heterogenotes formed from sexduction by an F' donor. Following the mating of F' W4520, carrying an F' gal₁⁺ gal₂⁺ factor, with W3350, a gal₁⁻ gal₂⁻ recipient, a number of recombinant clones were picked and examined for...
Resource Type:: Masters Thesis
Full Text:: , 1967 Typed by Donna Olson for Carole Mahaney Kurahara ACKNOWLEDGMENTS What we easily learn, we

Stable isotope probing of the ovine rumen for RDX degrading microorganisms

Creator:: Mitchell, Edward A.
Abstract:: The anaerobic and highly reductive conditions found in the ovine rumen are considered favorable for the degradation of the nitroaromatic explosives such as hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). In this study, we used stable-isotope-probing to determine the bacteria responsible for the degradation of RDX in the rumen. Results indicate 10 μg mL⁻¹ (45...
Resource Type:: Masters Thesis
Full Text:: differentiate the labeled and unlabelled DNA. To obtain 13C-DNA, E. coli DH5α was grown on uniformly labeled

A bacterial disease of the American shad (Alosa sapidissima)

Creator:: Rothman, Stanley
Abstract:: For the past six years a bacterial infection has been the cause of large losses of adult, spawning, American shad (Alosa sapidissima) in the Coos, Millicoma and Smith Rivers of Oregon. There was a sizable commercial fishery for shad in these rivers and losses of fish due to this infection...
Resource Type:: Masters Thesis
Full Text:: Olson for Stanley Rothman Redacted for Privacy Redacted for Privacy Redacted for Privacy

Microbiology of basalts targeted for deep geological carbon sequestration : field observations and laboratory experiments

Creator:: Lavalleur, Heather J.
Abstract:: With rising concentrations of CO₂ in the Earth's atmosphere causing concern about climate change, many solutions are being presented to decrease emissions. One of the proposed solutions is to sequester excess CO₂ in geological formations such as basalt. The deep subsurface is known to harbor much of the microbial biomass...
Resource Type:: Masters Thesis
Full Text:: : A Handbook of Physical Restraints, American Geophysical Union. Boyd E, Cummings D, Geesey G

Growth rate effects during starvation-survival of a marine psychrophilic vibrio

Creator:: Moyer, Craig Lee
Abstract:: Cell populations of the marine bacterium ANT-300, from either batch culture or continuous culture with dilution rates ranging from D = 0.170 h⁻¹ (fast) to D = 0.015 h⁻¹ (slow) were monitored for 98 days under starvation. Viability (CFU), acridine orange direct counts (AODC), and optical density were measured. DNA,...
Resource Type:: Masters Thesis
Full Text:: population; the vertical bars represent the standard error of the mean. 7.00 6.00 ro E 5.00 `--1 4.00 D

High resolution digital imaging of bacterial cells

Creator:: Siebold, William A.
Abstract:: The most abundant clone found in ribosomal RNA clone libraries obtained from the world's oceans belongs to the SAR11 phylogenetic group of environmental marine bacteria. Imaging and counting SAR11 bacterial cells in situ has been an important research objective for the past decade. This objective has been especially challenging due...
Resource Type:: Masters Thesis
Full Text:: random target 30.0 V 0 E 20.0 0 0a 0 0.0 0 four million base-p aIr genome - one million base-pair

Construction of a model organism for performing calcium carbonate precipitation in a porous media reactor

Creator:: Kaufman, Megan J.
Abstract:: Aquifers are an important storage location and source of fresh groundwater. They may become polluted by a number of contaminants including mobile divalent radionuclides such as strontium-90 which is a byproduct of uranium fission. A method for remediating such divalent radionuclides is sequestration through co-precipitation into calcium carbonate. Calcium carbonate...
Resource Type:: Masters Thesis
Full Text:: E. coliDH5α(pURE14.8) were as follows:…………………………………………………………………... 99 LIST

Methionine recycling in fruit studies on the metabolism and function of selected intermediates

Creator:: Gander, Lise Marie K.
Abstract:: This research has focussed upon both the in vitro and in vivo characterization of the recycling pathway leading from 5'-deoxy-5'-methylthioadenosine to methionine in fruit. In addition to contributing towards polyamine biosynthesis and other important cellular functions, in plant tissues these events also serve to generate the hormone ethylene. A broad...
Resource Type:: Masters Thesis
Full Text:: ). The effect also has been observed in LDC mutants of E. coli (49), suggesting two general paths for

The exploitation of methylthioadenosine phosphorylase deficiency in cancer therapy

Creator:: Lindburg, Katherine L.
Abstract:: Methylthioadenosine (MTA) is a naturally occuring nucleoside formed during the synthesis of polyamines in mammalian cells. MTA is rapidly catabolised by MTA phosphorylase (MTAase), eventually yielding methionine and adenine. MTAase has been shown to be active in all normal cells, but deficient in some cells and tissues of malignant origin....
Resource Type:: Masters Thesis

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