Animals must manage interactions with beneficial as well as detrimental microbes. Immunity therefore includes strategies for both resistance to and tolerance of microbial invaders. Transforming growth factor beta (TGFβ) cytokines have many functions in animals including a tolerance-promoting (tolerogenic) role in immunity in vertebrates. TGFβ pathways are present in basal...
An important question in the study of Riemannian manifolds of positive sectional curvature is how to distinguish manifolds that admit a metric with non-negative sectional curvature from those that admit one of positive curvature. Surprisingly, if the manifolds are compact and simply connected, all known obstructions to positive curvature are...
Many industrially important reactions use immobilized enzymes in non-aqueous, organic
systems, particularly for the production of chiral compounds such as pharmaceutical precursors.
The addition of a spacer molecule (“tether”) between a supporting surface and enzyme often
substantially improves the activity and stability of enzymes in aqueous solution. Most “long”
linkers...
Raman spectra distributed temperature sensing (DTS) by fiber-optic cables has
recently shown considerable promise for the measuring and monitoring of surface and
near-surface hydrologic processes such as groundwater–surface water interaction,
borehole circulation, snow hydrology, soil moisture studies, and land surface energy
exchanges. DTS systems uniquely provide the opportunity to monitor...
Climate models differ in their responses to imposed forcings, such as increased greenhouse gas concentrations, due to different climate feedback strengths. Feedbacks in NCAR’s Community Atmospheric Model (CAM) are separated into two components: the change in climate components in response to an imposed forcing and the “radiative kernel,” the effect...
Abstract Endogenous levels and signaling of gibberellin plant hormones such as
gibberellic acid (GA) have been genetically down-regulated to create semi-dwarf
varieties of poplar. The potential benefits of semi-dwarf stature include reduced risk of
wind damage, improved stress tolerance, and improved wood quality. Despite these
benefits, modification of growth traits...
Hypoxia is increasing in coastal zones worldwide, with acute effects on demersal fish and benthic invertebrate communities in shallow coastal and estuarine habitats. Less studied are the effects of hypoxia on planktonic larvae of open coastal habitats. Climate change projections suggest intensified hypoxia in open coast upwelling systems, such as...
To explain the dynamic behavior of the matric potential at the wetting front of gravity
driven fingers, we take into account the pressure across the interface that is not continuous
and depends on the radius of the meniscus, which is a function of pore size and the dynamic
contact angle...
Real-time PCR assays using locked nucleic acid (LNA) probes and high resolution melt (HRM) analysis were developed for molecular differentiation of Pseudoperonospora cubensis and P. humuli, causal agents of cucurbit and hop downy mildew, respectively. The assays were based on a previously identified single nucleotide polymorphism (SNP) in the cytochrome...
The majority of social science research is cross-sectional in nature, with data collected at a single point in time. However, social systems are dynamic and many of the variables of interest to social scientists may change over time. Longitudinal research methods enable data collection at two or more points in...
In the mid-2000s the U.S. west coast oyster industry experienced several years of significant production failures. This industry has been referred to as the “canary in a coal mine” for ocean acidification (OA). Industry-led collaboration with university and government scientists identified a relationship between elevated carbon dioxide in seawater and...
Desferrioxamine B (DFOB) was reversibly and covalently conjugated to polystyrene microspheres via N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP) crosslinker. DFOB retained post-immobilization activity with at least 89% able to bind Fe(III)-citrate under pH 7.4 and a citrate:iron molar ratio = 100:1. Comparing iron capture by DFOB-activated beads and sulfhydryl (−SH) control beads demonstrated...
The relationship of running biomechanics, footwear, and injury has been studied extensively in adults. There has been little research on the effects of footwear on running biomechanics in youth.
Groundwater–surface-water (GW-SW) interactions in streams are difficult to quantify because of heterogeneity in hydraulic and reactive processes across a range of spatial and temporal scales. The challenge of quantifying these interactions has led to the development of several techniques, from centimeter-scale probes to whole-system tracers, including chemical, thermal, and electrical...
Phytophthora infestans, the cause of the devastating late blight disease of potato and tomato, exhibits a clonal reproductive lifestyle in North America. Phenotypes such as fungicide sensitivity and host preference are conserved among individuals within clonal lineages, while substantial phenotypic differences can exist between lineages. Whole P. infestans genomes were...
Aims:
To design and validate a colorimetric loop-mediated isothermal amplification assay for rapid detection of Phytophthora infestans DNA.
Methods and Results:
Two sets of loop-mediated isothermal amplification (LAMP) primers were designed and evaluated for their sensitivity and specificity for P. infestans. ITSII primers targeted a portion of the internal transcribed...
n situ soil moisture monitoring networks are critical to the development of soil moisture remote sensing missions as well as agricultural and environmental management, weather forecasting, and many other endeavors. These in situ networks utilize a variety of sensors and installation practices, which confounds the development of a unified reference...
Ecological restoration programs often attempt to maintain or enhance ecosystem services (ES), but fine-scale maps of multiple ES are rarely available to support prioritization among potential projects. Here we use agency reports, citizen science, and social media as data sources to quantify the spatial distribution of five recreational elements of...
Phytophthora infestans, the causal agent of late blight disease, has been reported in North America since the mid-nineteenth century. In the United States the lack of or very limited sexual reproduction has resulted in largely clonal populations of P. infestans. In 2010 and 2011, but not in 2012 or 2013,...
