Salmonid B lymphocytes were stimulated both in vivo and in vitro
and shown to produce antibody specific for the immunogen. A passive
hemolytic plaque assay was employed to demonstrate B cells producing
specific antibody. Characterization of plaque forming cells (PFCs)
involved sequential inhibition with a series of graded concentrations
of...
The in vitro effects of cortisol in regulating salmonid B cell functions were
investigated. B cells at three distinct stages of differentiation were examined for
cortisol sensitivity. B lymphocyte responses were examined 1) during the early stage
of the precursor, 2) during the intermediate stage of differentiation associated with
clonal...
The concentration of ascorbic acid in the plasma was determined on
four levels of ascorbic acid intake for seven day periods in 1946-47 and
on three levels of ascorbic acid intake for ten day periods in 1947-48.
The subjects, eight girls and eight boys, were 16 to 19 year old...
Currently little is known about the mechanisms and locations of
lymphocyte development in teleosts. In this study several aspects of the
underlying factors which govern B lymphocyte development in trout
were investigated which included: the isolation and characterization of
immunoglobulin heavy chain (IgH) genes, the recombination activating
genes 1 and...
Ascorbic acid, or vitamin C, is well known as a co-factor for proline-hydroxylase and as an anti-oxidant. However, it is also capable of forming covalent bonds, particularly in the role of a nucleophile, henceforth termed 'ascorbylation'.
The ascorbylation of electrophiles can occur under physiological conditions. Furthermore, there are a number...
Phospholipase C-β (PLC-β) isozymes are key effectors in G protein-coupled
signaling pathways. Prior research suggested that some isoforms of PLC-β may exist
and function as dimers, but little is known about dimerization of PLC-β. Data from coimmunoprecipitation
assays of differentially-tagged PLC-β constructs and sizeexclusion
chromatography of native PLC-β support homodimerization...
Phospholipase C-Jβ (PLCβ) is an important effector enzyme in G-protein-coupled
signaling pathways. Activation of PLCβ by Gα and Gβy subunits has been fairly well
characterized, but little is known about other protein interactions that may also
regulate PLCβ function. A yeast two-hybrid screen of a mouse brain cDNA library
with...
The purpose of this study of the life and career of Dr. Carl
Leonard Anderson was to recount his professional contributions to
the field of health and relate these to public health and health education. The major emphasis of this study concerned Dr. Anderson's
professional life.
The accomplishment of the...
Chronic hepatitis B disproportionately affects Asian Americans. Although Asians make up only 4.2% of the total United States (U.S.) population, they account for more than half of those infected with hepatitis B. If left untreated, chronic hepatitis B could lead to cirrhosis, liver damage, and liver cancer. Studies show that...
Vitamin C is an essential component of the human diet. Uptake of vitamin C in the cell is regulated by the function of sodium-dependent vitamin C transporters (SVCTs). SVCT1 (encoded by the SLC23A1 gene) is expressed in the colon, liver and kidney, where it plays a vital role in determining...
L. C. Dunn (1893-1974) spent most of his scientific career conducting research in
developmental genetics as a member of the Zoology Department at Columbia
University in the City of New York. He had an accomplished scientific career
researching mutations in mice, which earned him respect from other geneticists and
scientists....
Oxidative stress is the major driving force behind the aging process and many age-related diseases. However, direct experimental evidence of whether antioxidants, such as ascorbate (AA) and lipoic acid (LA) can slow the progression of aging process and/or reduce risks of developing degenerative disease is largely absent. This suggests a...
Previous studies have found that smoking may have a negative effect on
vitamin B-6 indices and have demonstrated a possible association between smoking
and depressed plasma pyridoxal-5'-phosphate (PLP) concentration. Individuals with
plasma PLP values below the adequate level of 30 nmoles/L might benefit from
consumption of vitamin B-6 supplements, but...
Although much has been written describing the
various reactions to the origin of Species in America, the Mormon reaction to Darwinism has been largely ignored. This dissertation will recount the history of this reaction as exemplified by the life and works of B. H. Roberts. Roberts's intellectual pursuits early in...
DNA microarray technology is a powerful tool for analyzing patterns in gene expression data for thousands of genes. Due to a number of systematic variations in microarray experiments, the raw gene expression data is often obfuscated by undesirable technical noises. Various normalization techniques were designed in an attempt to remove...
Bromoalkoxylation of dihydropyran with bromine and 1-borneol, followed by dehydrobromination and epoxidation, afforded trans-2-1- bornyloxy-3,4-epoxytetrahydropyran as a major product. This was treated with sodium phenylselenide to give a 1:1 mixture of (2R,3R,4R)- 2-1-bornyloxy-3-hydroxy-4-phenylselenotetrahydropyran and its (2S,3S, 4S) diastereomer. Elimination of the selenoxide, derived from oxidation of the former with hydrogen...
Amphidinolide B₁ is one of the most potent cytotoxic compounds in the
amphidinolide family. Its bioactivity and intriguing structure has drawn
attention from synthetic chemists. Although numerous synthetic efforts toward
the total synthesis of amphidinolide B₁ have been published, it still remains an
unconquered synthetic target.
One of the unique...
Spatially explicit maps of habitat relationships have proven to be valuable tools for conservation and management applications including evaluating how and which species may be impacted by large scale climate change, ongoing fragmentation of habitat, and local land-use practices. Studies have turned to remote sensing datasets as a way to...
A continuous process for the production of ceramic materials has been studied.
This method reacted metal and oxidizer in a diffusionally controlled process,
demonstrated by reacting a magnesium particle stream and hot water vapor. Many small
rich hydrogen/air diffusion flames provide an atmosphere of hot water vapor, hydrogen,
and nitrogen...
Calls for reform within engineering education frequently cite the need to better prepare the future workforce for the imminent challenges that they will face. The focus in preparing students for the workforce is often on the educational system and not on understanding the actual workplace. But if we are to...
The use of Landsat data has historically been constrained to spectral and spatial information derived from a carefully selected image or set of images. However, free and open access to Landsat imagery combined with advances in data storage and computing are revolutionizing how the Landsat temporal domain is used to...
The purpose of this study was to investigate the impact of youth
gang awareness training on school personnel. Middle and high school level
teachers, counselors, and school administrators in a rural Oregon school
district were the focus of this study.
Six specially designed modules were created by the researcher and...
Racial integration of the Louisiana public school system had a devastating
effect on its number of Black teachers. The state has yet to recover from this
reduction, as fewer Black college students pursue education degrees. This study
reports on whether or not the lack of Black educators has influenced high...
The objective of this study was to compare the effects on eating behavior,
lipids, lipoproteins, lipid peroxidation, and glycemic control in women with type 2
diabetes of a high-monounsaturated fat diet (HM) compared to a high-carbohydrate
diet (HC).
In an outpatient feeding study, ten hypertriglyceridemic postmenopausal type
2 diabetic women...
A spectral investigation of the molecular vibration region of
the potassium sulfate crystal system was undertaken. This study was
initiated to establish the feasibility of using this crystal as a
host matrix for ammonium sulfate. Since these two systems are isomorphic
and have unit cells of nearly equal volume the...
Fluid-structure interaction (FSI) is an interesting and challenging interdisciplinary area comprised of fields such as engineering- fluids/structures/solids, computational science, and mathematics. FSI has several practical engineering applications such as the design of coastal infrastructure (such as bridges, levees) subjected to harsh environments from natural forces such as tsunamis, storm surges,...
Leadership, organizational structure, and communication/interpersonal relations are key functions studied. Evaluation of the Interior Columbia Basin Ecosystem Management Project (Project) considers how well those factors were managed by Forest Service and Bureau of Land Management in a bioregional assessment during a period from November, 1994 to November, 1995. Exploration of...
Aflatoxins B₁, Q₁ and aflatoxicol, R [subscript o] F, but not B₂ were activated in the presence of NADPH by the 105,000 x g pellet from rainbow
trout (Salmo gairdneri), Mt. Shasta strain, liver to products lethal to
Bacillus subtilis GSY 1057 (metB4, hisAl, uvr-1). Electron microscopy
confirmed the microsomal...
Atlantic menhaden, compared to gulf menhaden, exhibit
a life history pattern that appears to be adapted to a more
unpredictable reproductive environment. From experiments
on laboratory-reared eggs and larvae, Atlantic menhaden have
larger eggs with larger yolk volume. They are larger at
hatching, utilize their yolk faster, begin feeding at...
The structural architecture present in marine toxin azaspiracid - 20 stereocenters, 9 rings, 3 separated spirocenters - has attracted considerable synthetic attention. Our efforts toward the synthesis of azaspiracid have led to the completion of both C₁-C₂₆ northern and C₂₇-C₄₀ southern halves. Herein, the
synthesis of southern FGHI ring system...
This investigation consisted of two parts: A. stability, B.
bioavailability of vitamin B₆ in wheat. Three variables; whole wheat
flour (WHW), white flour (W) and W enriched with vitamin B₆ (WB₆)
were tested.
Stability of vitamin B₆ during bread making and storage of
bread and flour was determined. Bread was...
The vitamin B₁₂ concentrations in both the water and sediments
of Klamath Lake were found to vary significantly during a
one-year period. The patterns of B₁₂ distribution were remarkably
similar among the three main sites. In all cases there was an extremely
sharp gradation in concentration in relation to depth...
Serine transhydroxymethylase (STHM), a pyridoxal 5'-
phosphate requiring enzyme is indirectly involved in
pyrimidine deoxynucleotide metabolism. A decrease in the
activity of this enzyme could lead to altered deoxycytidine
(dC) metabolism. This study was undertaken to determine if
a vitamin B-6 deficiency affects dC metabolism. The effect
of a vitamin...
Child care stability affects child and family outcomes. Stability reflects the time dimension of a child care arrangement. Although stability does not guarantee positive outcomes, instability appears to decrease the likelihood of achieving them. Some level of stability is a necessary, although not sufficient, characteristic of care that meets children's...
Global increases in ultraviolet-B radiation (UVBR:290-
320 nm) resulting from decreases in stratospheric ozone
concentration have the potential to alter marine primary
production and affect global climate and marine trophic
dynamics. Effects of UVBR on phytoplankton carbon fixation
were determined from open ocean exposure studies conducted
off the coast of...
The purposes of the research presented in this thesis were: (1)
to determine the precision with which it is possible to measure
changes in vitamin B6 compounds in the blood and urine following
oral doses of levels of vitamin B6 (as pyridoxine) which are in the
range of the normal...
Current dietary recommendations have placed increasing emphasis on dietary fat
as an important element to decrease risk of cardiovascular disease (CVD). Although total
fat and the fatty acid composition of diets influence the risk of CVD, the optimal amounts
of different fatty acids are not well defined, especially if n-6...
Four studies were conducted to evaluate the effect of varying levels of vitamin
B-6 (B6), protein and pyridoxine glucoside (PNG) on B6 status and requirements of
women. In the first two studies, women were fed a constant protein diet and vitamin
B-6 intakes of 0.84 to 2.39 mg/d during 10-...
Fire is the dominant disturbance process in western U.S. forests, and although effects of fire in upland forests are relatively well-studied, there is little information about fire effects on riparian forests, critical areas of the landscape for both habitat and water quality. This dissertation examines different aspects of fire effects...
The mangrove ecosystem has served as a life-support system to large populations of coastal dwellers in Ecuador for many generations. Diverse communities comprised of multi-racial and multi-ethnic groups have formed along the edge of the mangrove forests throughout the Ecuadorian coast. These groups self-identify as being part of an “ancestral”...
A series of experiments was designed to investigate differential
flood-tolerance and the phenomenon of flood-induced stomatal closure
of several fruit tree species. The first two experiments were conducted
with potted plants submerged outdoors 5-10 cm above the soil line.
Plant morphology, growth, leaf conductance (cL), and soil oxygen diffusion
rate...
Prostate cancer risk is significantly lower in Asian countries compared to the US, which has prompted interest in the chemo-preventative action of dietary components such as soy and green tea commonly found in Asian diets, such as soy and green tea. Studies have suggested that soy isoflavones and green tea...
The competition or rivalry for the use of water resources
among economic sectors of the Pacific Northwest and among geographical
regions of the western United States has intensified in recent
years. This rivalry and the long run prospects for water shortages
have increased the demand for research concerning the productivity...
Prostaglandin F₂α (PGF₂α), a stimulatory hormone of luteal oxytocin
secretion, is known to activate protein kinase C (PKC); however, the
intracellular signals that promote exocytosis of oxytocin remain to be
elucidated. Myristoylated alanine-rich C kinase substrate (MARCKS), a
protein specifically phosphorylated by PKC, crosslinks actin filaments
associated with the inner...
The competition or rivalry for the use of water resources
among economic sectors of the Pacific Northwest and among geographical
regions of the western United States has intensified in recent
years. This rivalry and the long run prospects for water shortages
have increased the demand for research concerning the productivity...
Studies were directed toward the formulation of a selective
Aeromonas medium that would permit the isolation of Aeromonas
species in the presence of other common bacterial flora. The final
composition of this medium, PBG Agar, in grams per liter, is:
Bacto-peptone, 10; Bacto-beef extract, 10; glycogen, 4; NaCl, 5;
sodium...
Invasive plants have the potential to reduce the diversity of species in plant and animal communities. I examined the negative effect of two invasive wetland plants, purple loosestrife and reed canary grass, on the species richness and diversity of plant and moth communities within 24 wetland study sites in the...
School districts across the nation are engaged in an ongoing attempt to bring about
change. Educational researchers strive to initiate change in the classroom with students as
well as the teachers of these students.
The evaluation of a change effort is a key element in any implementation model; as
the...
Consider the c- sample testing problem with null hypothesis H[subscript]o : F₁(x) = ... = F[subscript]c(x), where F[subscript]j(x), j = 1 ..., c, represent
absolutely continuous distribution functions for failure distributions.
Furthermore, consider arbitrarily (right) censored samples, which
may arise from putting the (censored) experimental units on test at
different...
Chlordecone belongs to the class of persistent organochlorine pesticides that are remarkably resistant to environmental degradation. Even though their use was banned in the United States in 1978, these compounds can still be detected in both humans and wildlife throughout the world. Previous work has shown that the pretreatment of...
Rainbow trout cystatin C cDNA has been isolated from
trout liver. The full-length cystatin cDNA (674 bp)
included the 5'untranslated region and the polyadenylation
signal sequence AATAAA in the 3' region. Translation of
the cDNA defines 132 amino acid residues. Comparison of
the amino acid sequence with those of family...
In situ, reduction of Cp*ReO₃ by PPh₃ to form (Cp*ReO)₂(μ-O)₂ allows catalytic deoxygenation of epoxides, however, conproportionation between Reⱽ and Reⱽᴵᴵ species to form clusters of ((Cp*Re)₃(μ-O)₆}²⁺(ReO₄⁻)2 and new compound {(Cp*Re)₃(μ²-O)₃(μ³-O⁾³ReO₃}⁺(ReO₄⁻) leads to removal of rhenium from the catalytic cycle and loss of activity, The epoxide deoxygenation mediated by (Cp*ReO)₂(μ-O)₂...
Early behavioral regulation has been identified as an important component of school readiness, yet few easy-to-administer measures exist that directly assess this skill in young children outside of the United States (U.S.). In this dissertation, two studies were conducted to examine the reliability and validity of a direct measure of...
Effects of tanoak (Lithocarpus densiflorus) on early
growth, morphology, and physiology of Douglas-fir
(Pseudotsuga menziesii) were studied to determine
developmental characteristics of young stands and
mechanisms of competition. Growth, leaf area, and biomass
of Douglas-fir, tanoak, and shrubs / herbs were monitored
for six years after establishing a gradient of...
The dissertation uses state-of-the-art structural engineering theories, in parallel with the modern and still-developing control theory of electrical engineering to mitigate the vibratory responses of a double-stayed bridge subjected to strong earthquake ground motions.
First, a nonlinear finite element model of the double-stayed bridge subjected to non-uniform (multi-support) earthquake excitations...
Despite recognition in the literature that adolescence
represents a relatively high-risk developmental period for
health-risk behaviors, only limited attention has been given
to the subjective meanings adolescents assign to such
behaviors. One potentially fruitful avenue to explore in
understanding the adolescent perspective on health-risk
behaviors is the use of word...
The species composition of soil microfungal populations in-adjacent
stands of red alder, conifers, and mixed alder conifer correlated
strongly with the dominant vascular vegetation. A total of 92
species were isolated: 55 from the alder stand; 45 from the conifers;
and 46 from the mixed alder-conifer, with few species (16,...
Randomized trials are the gold standard for the clinical assessment of a new treatment
compared to a placebo or standard of care. Often in clinical trials, patients are
accrued sequentially rather than all at once. Thus, the data from such a trial becomes
available sequentially to the researcher. Monitoring and...
The Earth’s surface is experiencing unprecedented change. Humanity’s growing population, expanding land-use footprint, and increasing global emissions of atmospheric greenhouse gases affect a vast number of species on Earth and the functioning of virtually all ecosystems. Given the vital interactions and feedbacks between the Earth’s land surface and climate, measurements...
The problem of the study was to determine whether university students
enrolled in a pre-service teacher training course and exposed to a group
counseling procedure having as its major emphasis education in the affective
domain could demonstrate a significant change in self-actualization
when compared with similarly enrolled students who had...
Purpose of the Study
The purpose of this thesis is to survey the current practices in
guidance services in the public secondary schools of British Columbia
and on the basis of the survey findings, together with a review of the
literature of guidance, to present recommendations for a feasible
program...
Voltage controlled oscillator (VCO) based ADC is an important class of time-domain ADC that has gained widespread acceptance due to their several desirable properties. VCO-based ADCs behave like an open-loop continuous time ΔΣ modulator and achieve excellent resolution by first order noise shaping the quantization error. However, the SNDR of...
Two studies were conducted to determine the effect of varying vitamin B-6 (B- 6) status on lymphocyte mitogenic response and pyridoxal 5'-phosphate (PLP) concentration in peripheral blood mononuclear cells (PBMC) in young women. In the first study, women were fed 1 mg/d for the first week and 1.5, 2.1 and...
Trained male cyclists (6 in study 1, 5 in study 2) cycled to exhaustion (EXH) at
75% of VO₂ max twice; once in the non-supplemented (NS) state and once in the vitamin
B-6 (B-6)(20 mg PN) supplemented (S) state. The diet contained 2.3 mg B-6 in study 1
and 1.9...
The numerous different types of nanomaterials and paucity of reliable in vivo information on toxicity has resulted in enduring uncertainties associated with health and safety risks, thereby slowing progress in nanotherapeutic assessment and development. This dissertation explores how the embryonic zebrafish model can be applied to prioritize commonly used nanomaterial...
The importance of physical activity is well documented (Haskell et al., 2009a; McReynolds & Rossen, 2004; Steinbeck, 2001). Despite physical activity’s benefits, many individuals are not meeting current weekly physical activity guidelines recommending ≥ 150 minutes of moderate or ≥ 75 minutes of vigorous aerobic physical activity and 2 or...
The spread of nonnative species across the globe has contributed to biodiversity loss and changes in ecosystem structure and function. Monitoring the introduction, naturalization, and spread of introduced species is critical in abating negative impacts wrought by species invasions. However, providing basic information concerning the presence or spread of many...
Pacific sardines (Sardinops sagax) are an economically and ecologically important forage fish which transfer energy from planktonic primary producers and secondary consumers to upper trophic predators. Previous genetics studies of Pacific sardine suggested a panmictic population with a shallow genetic structure. However, more than one subpopulation within the Central California...
Wave dispersion and fracture are phenomena that involve. mechanisms triggered at the scale of a material's internal structure. Experiments were performed with a candidate material to judge the hypothesis that wave dispersion measurements can be used to nondestructively predict fracture strength. Wood-based particleboard was chosen as the candidate material because...
A study consisting of three parts was undertaken to investigate how variation in species interactions, population genetic structure, epidemiological parameters, and plant breeding system may influence the ecology and biological control of an apomictic invasive plant, Chondrilla juncea (Asteraceae). Interactions between natural enemy species may modify their net effect on...
Premature birth interrupts the typical development of the human fetus, leaving the infant born with underdeveloped gastrointestinal and immune systems. Preterm infants have reduced stomach acidity, reduced digestive protease activity, more permeable intestinal membranes, impaired innate and adaptive immune response, and poor microbiome development. Due to these factors, preterm infants...
The (nonlinear) response behavior of a piecewise-linear system is investigated under
both deterministic and stochastic excitations in this study. A semi-analytical procedure is
developed to predict the system behavior by evaluating the joint probability density functions
of the Fokker-Planck equations of the stochastic piecewise-linear system under random
excitations via a...
Complex nonlinear and chaotic responses have been recently observed in various
compliant ocean systems. These systems are characterized by a nonlinear mooring
restoring force and a coupled fluid-structure interaction exciting force. A general class
of ocean mooring system models is formulated by incorporating a variable mooring
configuration and the exact...
Ever since composite laminate technology was introduced into the aerospace and automotive industry, there has been a need to fully understand the damage progression experienced by composite laminated plates in the presence of a notch. While numerous research studies have been conducted on this matter when subjected to in-plane loads,...
Wheat sharp eyespot (SES), caused by the soil-borne fungus Rhizoctonia cerealis is a common stem disease of wheat globally. The disease caused a severe and extensive epidemic throughout the Willamette Valley of Oregon in 2014 and has remained one of the most important wheat diseases in this region. However, litte...
A simulation method using a hybrid computer is developed in
this dissertation to enhance the study of wind-driven ocean circulation.
The simulation is based on the mathematical model developed by
Ekman. Pure digital simulation is not economical due to excessive
computer time required for the solution of the partial differential...
Studies were initially conducted to determine the action of
intravenous serotonin on perfused segments of human umbilical
cords in vitro. Intravenous serotonin, via bolus injection or
continuous infusion, was found to elevate venous perfusion pressure,
while intra-arterial serotonin failed to alter venous pressure.
Norepinephrine, administered to the venous perfusate, was...
Dietary protein intake has been observed to influence the induction
of cancer in animals exposed to carcinogens, presumably by affecting the
activities of enzyme systems which metabolize them. One such carcinogen
is aflatoxin B₁ (AFB₁) a mold metabolite and frequent contaminant of
foodstuffs. Variations in the levels of dietary proteins...
To investigate the responses of healthy adult women to two
levels of vitamin B-6, a seven week two-part experiment was conducted
with five young adults and eight middle-aged subjects. During the
four unsupplemented weeks, dietary vitamin B-6 was 2.3-2.4 mg/day.
In the final three weeks, the same diet was supplemented...
The question of how the presence of nucleosomal
packing of DNA modifies carcinogen interaction at
specific sites cannot be answered by studies on whole
chromatin or bulk nucleosomes because of the
heterogeneity of DNA sequences in the particles. This
problem was circumvented by constructing nucleosomes that
are homogenous in DNA-histone...