Fungi are capable of growth on a wide variety of carbon sources, both living and dead. They can produce an arsenal of enzymes and transporters for harvesting sugars, polysaccharides, amino acids, lipids and micronutrients from their environments [1]. Within the nucleus of a cell, transcription factors (TF) control whether genes...
Flaxseed is a rich source of alpha-linolenic acid (ALA). Feeding laying hens flax seed can increase n-3 fatty acids content (FA) in eggs, thus increasing human intake of n-3 FA. However, non-starch polysaccharides (NSP), which decreases digestibility of dietary lipids and proteins. Addition of carbohydrase enzymes to flax-based layer diets...
In broiler chickens, hepatic lipid metabolism plays a significant role in whole body and muscle fatty acid (FA) incorporation. Flax seed is a rich source of α-linolenic acid and can be used to increase n-3 FA in poultry meat. Previous studies have shown that flax seed in combination with carbohydrase...
Nonalcoholic fatty liver disease (NAFLD) is characterized by the accumulation of excess neutral lipids within the parenchymal cells of the liver. The primary etiology is diet-induced and most often exists as a comorbidity with obesity, metabolic syndrome, and/or Type 2 Diabetes. The development of excess liver lipid can be self-limiting...
Flax seed is a rich source of alpha-linolenic acid (ALA). Feeding broiler birds flax seed can increase n-3 fatty acids (FA) in meat tissues and can increase human intake of n-3 FA. However, non-starch polysaccharides (NSP) in flax seed decrease digestibility of lipids and proteins and have a negative impact...
Although fish are a nutritious food source, they also are the main source of methylmercury exposure in U.S. populations. This research examined the risks from methylmercury and benefits from omega-3 fatty acids and selenium from fish consumption. The first study provided the first region-specific quantitative risk/benefit analysis for nine commonly...
The effects of different nitrogen sources on the primary and secondary pathways of Azotobacter vinelandii cells were studied by means of the radiorespirometric method. C¹⁴ labeled glucose and several key amino acids related to the tricarboxylic acid cycle, such as glutamic acid, aspartic acid, alanine, serine and glycine were used...
Nutritional studies with Acetobacter suboxydans indicated that
when isoleucine, lysine, methionine, serine and arginine were singly
omitted from a complete amino acid mixture, no growth was obtained.
However, the organism could grow on single amino acids like glutamic
acid, histidine and proline. No growth was obtained with ammonium
sulfate as...
Comparatively little work has been done on bacterial lipids.
There are profound differences between bacterial lipids in general
and lipids of the higher forms of life in such respects as the absence
of sterols, phospholipids low in nitrogen but high in inositol
and carbohydrate, the presence of large proportions of...
This investigation was undertaken to develop a procedure
by which the fatty acids could be separated from the
hydroxy fatty acids of bark waxes. The specific hexane-insoluble, benzene-soluble wax used in this research was obtained
from the bark of white fir, Abias concolor, (Lindl.
and Gord.).
The acids were removed...
In this study subcutaneous, inter- and intramuscular fats from 42
Hereford steers were analyzed for their fatty acid composition using
gas-liquid chromatography. The same tissues were analyzed from one
pair each of identical twins of Angus and Shorthorn breeding. Muscle
and liver lipids from these latter four animals were fractionated...
Routine studies of bacterial fatty acid and lipid composition,
made possible by recently developed techniques, have brought about
an increasing interest in the physiological significance of these
compounds. The purpose of this study was to characterize the fatty
acids of the vegetative cells of Clostridium botulinum 33A, as the
first...
The difficulties encountered in working with micro-quantities
of lipids have been overcome by the advent of new and improved
procedures in chromatography which have made possible better
fractionation and identification. Bacterial lipids because of their
different lipid pattern from other life forms have stimulated much
interest. Studies have been made...
Female Sprague-Dawley rats (220-240 g) were ovariectomized
and seven days later were either treated as controls or received a
single injection of either 0.1 μg, 100 μg, or 2 mg of estradiol-17β dissolved in 0.1 cc propylene glycol. These rats were autopsied
either 1, 3, 6, 24, 48, or 72...
The transport response of four marine psychrophilic bacteria
to a variety of substrates was determined and related to environmental
conditions. Studies on pressure and temperature effects on glutamic
acid transport and utilization indicated that high pressures and
low temperatures inhibit glutamate transport, while permitting glutamate
respiration. Similar results were obtained...
Developing steelhead trout eggs and sac fry were examined for
wet, dry and lipid weights and for fatty acid composition over the
period from fertilization until the disappearance of the yolk sac, 69
days after fertilization. The yolk sac and embryo or fry were
separated shortly before hatching, and the...
Non-alcoholic fatty liver disease (NAFLD) is defined as excess hepatic lipid accumulation, in the absence of excess alcohol consumption and chronic liver disease. NAFLD can range in severity from simple fatty liver (steatosis) to non-alcoholic steatohepatitis (NASH). NASH is defined as hepatic steatosis with inflammation and hepatic injury and describes...
During the 21 day incubation period, the fertile egg provides nutrients such as fatty acids for energy and polyunsaturated fatty acids (PUFA) for membrane synthesis to the developing chick. The hypothesis tested in the present study is that the type of PUFA fed to the breeder hen can alter tissue...
We have previously shown that diets enriched with (n-3) fatty acids reduced the delayed-type hypersensitivity (DTH) skin reaction to keyhole limpet haemocyanin (KLH) in geriatric-Beagles. Although the amount of α-tocopheryl acetate in diets of the previous study exceeded requirements, plasma α-tocopherol concentration was significantly lower in dogs fed the high...
The vascular system transports components essential to the survival of the individual and acts as a barrier to substances that may injure the organism. Atherosclerosis is a dynamic, lesion producing disease of the arterial system that compromises the functioning of the organ by occlusive and thrombogenic processes. This investigation was...
Type 2 diabetes mellitus (T2DM) is a disease of multi-complications
affecting more than 20 million US adults. Hyperglycemia is the classic clinical feature of diabetes, and uncontrolled hyperglycemia leads to deadly health complications. Thus, control of blood glucose represents a major goal for diabetics. Human and rodent studies revealed another...
Three experiments were conducted to study the influence of dietary fatty acids on the production performance and immune response of chickens. In experiment I, forty day-old broiler chicks were fed diets containing 5% of either animal fat + conjugated linoleic acid (CLA) (Diet I), sunflower oil (Diet II), flax oil...
Arachidonic acid (ARA) and docosahexaenoic acid (DHA) are polyunsaturated fatty acids required for proper embryonic development, specifically neurodevelopment. However, little is known regarding their conversion to other metabolites during embryogenesis. The oxidation of ARA gives rise to the biologically active eicosanoids and the oxidation of DHA gives rise to the...
Inflammation is the body's response to injury and is characterized by pain, swelling, redness, and heat. Eicosanoids are lipid mediators of inflammation. Leukotrienes (LT) are 20-carbon eicosanoids produced from arachidonic acid (AA), an n-6 fatty acid (FA), and eicosapentaenoic acid (EPA), an n-3 FA. LT produced from AA are proinflammatory...
The effect of pyridoxine (PN) supplementation on the activities
of erythrocyte alanine aminotransferase (EAlaAT) and aspartate aminotransferase
(EAspAT) was observed in five men, aged 22 to 25 years.
The subjects received a constant diet containing 1.34 mg of vitamin
B-6 Monday through Friday of each week during this five-week study....
Three studies were designed to evaluate the promoting
effect of cyclopropenoid fatty acids (CPFA) on avian myeloblastosis
virus (AMV) infection in White Leghorn chickens.
In the first study effects on growth parameters, blood serum
and erythrocyte lipid composition of White Leghorn chickens
were investigated at 100 ppm and 300 ppm...
Although it is well-established that the fatty acid profile of breast milk will
reflect the dietary fatty acids, the response time with which this occurs is not
known. We hypothesized that fatty acids from a given meal would be transferred
acutely from chylomicrons into breast milk. To test this hypothesis,...
Numerous dietary factors have been shown to influence the fatty acid profiles (FAP)
in breast milk from lactating women. However, few studies have evaluated the effect of trace
minerals on milk FAP. Consequently, the purpose of this study was to determine the effect
of selenium status on the FAP in...
Studies were conducted to determine the effects of cyclopropenoid
fatty acids (CPFA) on the microsomal membrane of livers of rainbow
trout (Salmo gairdneri). Slab and tube gel electrophoresis of
microsomes from trout fed a CPFA diet (CPFA-microsomes) for varying
time periods showed a decrease in the number of protein bands...
The effect of vitamin B-6 (B-6) status on plasma fatty acids (FA) levels and lipid
metabolism was investigated in this metabolic study. Eight female subjects were fed for 28
days. For the first 7 days, they were fed a constant diet containing 2.10 mg of B-6. For the
rest of...
Intrinsic characteristics of Pacific sardines were determined. The lipid content
in sardines was initially low (6.79%) in the beginning ofthe season, increased to
22.95% in mid-August, and decreased slightly by the end of September. An inverse
correlation (R² = -0.90) was found between lipid and moisture content. Analysis
showed that...
Cyclopropenoid fatty acids (CPFA), which are a group of
fatty acids produced by plants of the order Malvales, are known
to induce adverse physiological effects when administered to a
variety of animal species. A structurally strained cyclopropene
ring is present in all CPFA and is believed responsible for the
toxic...
Dietary cyclopropenoid fatty acids (CPFA) have long
been known to cause pronounced physiological disorders in
both farm and laboratory animals. Past work has shown
CPFA to be a powerful promoter of carcinogenesis in trout,
rats and mice. Sterculic and malvalic acids (CPFA's) are
found in seed lipids of plants from...
The effect of different blanching treatments and
packaging materials on the enzymatic (lipoxygenase and
peroxidase) activity and fatty acid stability of two
different varieties of sweet corn on the cob (Jubilee and GH
2684) was evaluated during nine months of frozen storage at
-23.3°C.
The initial moisture content in the...
The DNA repair capabilities of rainbow trout (Salmo gairdneri)
were studied vising the method of autoradiography. Trout were fed a
semi-purified control diet containing 0 ppm, 50 ppm, or 300 ppm
cyclopropenoid fatty acids (CPFA) for 6-9 weeks. Liver slices were
prepared and exposed in vitro to a control treatment,...
Cyclopropenoid fatty acids cause several physiological disorders
in rainbow trout and rats. Rainbow trout appear to be more sensitive
than rats to the effects of cyclopropenoid fatty acids. Carbon-14
labeled sterculic acid with label in the methylene position of the
cyclopropene ring was injected into the stomach of rats and...
Epidemiological studies have suggested that the consumption
of fish may reduce the risk of cardiovascular disease.
Compared to the number of studies using fish oils, few
studies have used fish itself. Those which have used fish
have generally used fattier fish such as mackerel and salmon
as part of an...
Cyclopropenoid fatty acids (CPFA) are natural components of
cottonseed oil, a major food oil in the United States. The ability of
CPFA to cause abnormal biochemical and physiological effects when
fed to laboratory and farm animals has prompted an investigation of
their effects on mice.
Between 0.05 and 0.55% CPFA...
In this five-week feeding study male New Zealand rabbits were
fed diets containing cyclopropene fatty acids (CPFA), diets containing
cholesterol, and diets with both CPFA and cholesterol added to test
the effect of CPFA on cholesterol metabolism and atherosclerosis
induction. When CPFA-fed animals were compared with control
animals, they tended...
The cyclopropene fatty acids, sterculic and malvalic, occur
naturally in a major food lipid, cottonseed oil, and have been implicated
in several physiological disorders. Rainbow trout in this
study were fed semipurified diets containing 200 or 300 ppm methyl
sterculate, and the effects on hepatic lipid and glycogen levels,
lipid...
Rainbow trout were fed semi-purified diets containing cyclopropenoid
fatty acids, and the biological effects of these acids were
investigated. These fatty acids occur naturally in cottonseed oil and
have been implicated in a large number of physiological disorders.
Included in this study was an investigation of the pattern of deposition...
The variation in fatty acid composition between the "bound" and
"free" lipid in stored anchovy meal was determined using gas-liquid
chromatographic analysis. A model system of fresh anchovy oil on
powdered lactalbumin was also studied to determine the changes in
extractability and fatty acid composition which occur during oxidation.
All...
The two naturally occurring cyclopropene fatty acids, sterculic
and malvalic, have been synthesized. The cyclopropene ring was
made by inserting a methylene unit into an acetylenic linkage.
The synthesis of methyl sterculate was repeated using diethyl
aluminum chloride, diazomethane, and methyl stearolate.
Methyl malvalate was totally synthesized. Diethyl malonate
was...
The purpose of this investigation was to modify the procedure
of Bills, Khatri and Day for use in the development of a suitable
method for determining the quantitative distribution of the free fatty
acids (FFA) in normal, heated, and rancid milk and milk fractions
The method consists of extracting the...
Two methods for the estimation of non-esterified fatty acids in
plasma were evaluated. An attempt was made to obtain a reliable
micro-technique to estimate these acids in blood fractions.
The titrimetric method of Trout et al. was satisfactory in the
analyses of pure fatty acids and fatty acids added to...
Current dietary recommendations have placed increasing emphasis on dietary fat
as an important element to decrease risk of cardiovascular disease (CVD). Although total
fat and the fatty acid composition of diets influence the risk of CVD, the optimal amounts
of different fatty acids are not well defined, especially if n-6...
Three studies were conducted to determine the effects of
cyclopropenoid fatty acids (CPFA) on the membrane components of livers
of rainbow trout (Salmo gairdneri). In the first study, ¹⁴C-sterculic
acid was administered by intraperitoneal injection into rainbow trout
and the trout maintained for 72 hours. The labelled sterculic acid
was...
There is growing public concern about the high proportion of saturated fatty acids in milk fat; however, feed intake, energy partitioning toward milk synthesis, and milk fat concentrations can decrease when cows are fed high concentrations of unsaturated lipids. The objective of this study was to identify the optimal rate...
Effect of diets varying in amount of cholesterol and
polyunsaturated to saturated fatty acid ratio (P/S) on plasma lipid
levels and lecithin:cholesterol acyltransferase (LCAT) activity was
determined in fourteen young adult male subjects during a 13-week
experimental period in a crossover design.
Control diet was fed for two weeks prior...
Medium chain fatty acids (MCFAs) are important industrial feedstocks for
soaps, detergents and surfactants. Interspecific lines derived from Cuphea lanceolata
and Cup hea viscosissima are being domesticated as a temperate source of MCFAs.
These species also serve as model organisms for understanding the biosynthesis of
MCFAs in seeds. Various markers...
In the presence of internal or external stressors, the body requires sources of energy that aide cells to combat inflammation. Fatty acids are an important source of energy and are vital components of cell membranes. Dietary fatty acids (n-6 and n-3) are of importance in immune function because they are...
Three experiments were conducted in broiler hens to study the influence of dietary n-3 polyunsaturated fatty acids (PUFA) on egg quality, antioxidant status in progeny, and eicosanoid production in tissue. The objective of experiment 1 was to determine the effect of hen age and dietary n-3 PUFA on egg quality...
Exercise increases B-vitamin (B6, B12, folate) dependent metabolic reactions, especially those related to energy production and the rebuilding and repairing of muscle tissue. These same B-vitamins are also important in maintaining low blood levels of homocysteine (Hcy), a cardiovascular disease (CVD) risk factor. Hcy levels rise when the metabolism of...
This research was designed to evaluate the Fatty Acid Signature (FAS) technique as a non-lethal alternative to more traditional, and sometimes destructive, methods of studying the diet composition of piscivorous birds. Specifically we tested the technique with Caspian terns (Hydroprogne caspia) which currently nest in large numbers in the Columbia...
A method based on fatty acid (FA) analysis is used to profile microbial community structure (MCS). Various extraction protocols are available, which alter the types of FAs extracted from soils. The more time consuming but widely used protocol extracts only FAs from phospholipids (PLFA). This technique is desirable because PLFAs...