Index Catalog // ScholarsArchive@OSU

Post-translational modifications of vaccinia virus proteins : molecular analysis of the myristylated L1R gene produc

Creator:: Ravanello, Monica P. G.
Resource Type:: Dissertation
Full Text:: L1R Ge Abstract approved: .6'17 Dr. Dennis E. Hruby Co-translational covalent attachment of

Pathogenic and molecular characterization of three closely related isolates of infectious pancreatic necrosis virus (IPNV)

Creator:: Bruslind, Linda D.
Abstract:: Three closely related isolates belonging to the A₁ serotype of infectious pancreatic necrosis virus (IPNV) were selected for comparison, to provide insight into the nature of variation in the virulence of IPN viruses. Brook trout fry (Salvelinus fontinalis) were experimentally infected with the three isolates by immersion. Cumulative mortalities over...
Resource Type:: Dissertation
Full Text:: committee members: Dr. Paul Reno, my major professor, Dr. Jo-Ann Leong, Dr. Rich Holt, Dr. Robert Olson

Distribution of Clostridium botulinum type E in fish, shellfish and the marine environment of the Pacific Northwest, and protein patterns of the toxigenic and non-toxigenic stains

Creator:: Craig, James Morrison
Abstract:: Interest in the distribution of Clostridium botulinum type E was heightened by the sudden outbreak of human botulism from smoked whitefish chubs and canned tuna fish in 1963. The question arose as to how widely the organism is distributed among fish and shellfish in the Northwest and what potential hazard...
Resource Type:: Dissertation
Full Text:: CLOSTRIDIUM BOTULINUM TYPE E IN FISH SHELLFISH AND THE MARINE ENVIRONMENT OF THE PACIFIC NORTHWEST, AND

Transmission of disseminated neoplasia in the soft shell clam, Mya arenaria

Creator:: House, Marcia
Abstract:: Disseminated neoplasia (DN) is a proliferative cell disorder that occurs in the circulatory system of bivalves. The condition is progressive and lethal. At least 15 species of bivalves over a wide range of geographic locations have been reported to contract DN. Prevalence levels of disseminated neoplasia can reach up to...
Resource Type:: Dissertation
Full Text:: privacy ACKNOWLEDGMENT I would like to thank the members of my committee, Dr. Paul Reno, Dr. Bob Olson

Development of bacteriophage inhibitory bulk starter medium for the cultivation of thermophilic lactic acid bacteria

Creator:: Rajagopal, S. N.
Abstract:: Internally-pH-controlled, phosphate containing and non-phosphate containing Italian bulk starter media were compared to reconstituted nonfat dry milk and commercial bulk starter media for their ability to support the growth and activities of commercially frozen thermophilic lactic acid cultures. Cultures grown in internally-pH-controlled media demonstrated superior acid-production capability. The cheese made...
Resource Type:: Dissertation
Full Text:: Bacteria Redacted for Privacy Abstract approved: William E. Sandine Internally-pH-controlled, phosphate

Studies on growth metabolites produced by Propionibacterium shermanii

Creator:: Salih, Mohammed Ali
Abstract:: The ability of Propionibacterium shermanii to produce growth metabolites which were inhibitory to food spoilage indicator organisms was investigated. Asperqillus niqer, a Pseudomonas species and Kluyveromyces fraqilis were the spoilage indicators chosen to represent molds, gram negative psychrotrophic bacteria and yeasts, respectively. Supplementation of milk or whey based media was...
Resource Type:: Dissertation
Full Text:: SHERMANII Abstract approved: Redacted for Privacy William E. Sandine The ability of Propionibacterium

Internal pH control starter media: use in culture preservation and for multiple strain growth

Creator:: Shigeno, Debra S.
Abstract:: Several bulk starter media, consisting of two experimental blends and four commercial preparations, were examined for each medium's ability to grow, preserve, and/or recover lactic streptococcal starter cultures. Nonfat milk was used as a control. Results showed that cultures which had the greatest survival rate in frozen storage had been...
Resource Type:: Masters Thesis
Full Text:: Privacy Dr. William E. Sandine Several bulk starter media, consisting of two experimental blends and

Studies on the inhibition of food-borne pathogens and spoilage bacteria by lactic acid starter cultures

Creator:: Daly, Charles
Abstract:: The ability of Streptococcus diacetilactis to inhibit a variety of food-borne pathogens and spoilage organisms in milk and broth cultures was demonstrated. Test organisms inhibited included Pseudomonas and Alcaligenes species, Eschericia coli, Salmonella, Staphylococcus aureus, Clostridium perfringens and Vibrio parahaemolyticus. In general, approximately 99.0% and 99.9% inhibition was observed in...
Resource Type:: Dissertation
Full Text:: STARTER CULTURES Abstract approved: Redacted for Privacy Dr. W. E. Sanctine The ability of

Studies to improve lactic acid bacterial starter cultures

Creator:: Khosravi, Lewa
Abstract:: Three commercial sausage starter cultures were grown in an internal-pH-controlled medium (PHASE 4) and in a soluble phosphate-buffered medium. The cultures were tested by comparing viable counts before and after freezing at -40 C or freeze-drying and subsequent storage for 3 months in the frozen or dried state. Strains varied...
Resource Type:: Dissertation
Full Text:: approved: Redacted for privacy William E. Sandine Three commercial sausage starter cultures were grown

Ecological study of aquatic myxobacteria in a woodland stream

Creator:: Carlson, René Virginia
Abstract:: Most studies of bacteria in water have concerned public health; little attention has been given to organisms which are indigenous to the aquatic environment. Myxobacteria are known to occur in soil, in the marine environment, and several have been studied in relation to diseases of salmonid fishes. However, little information...
Resource Type:: Masters Thesis
Full Text:: A WOODLAND STREAM Abstract approved: Redacted for Privacy kobert E. Pacha Most studies of

Characterization of thermophilic rod and coccus starter strains used in mozzarella cheese manufacture

Creator:: Faessler, Patrick Charles
Abstract:: The present investigation was undertaken to characterize a number of strains of Lactobacillus bulgaricus and Streptococcus thermophilus intended for use by a commercial starter supply company. Thorough characterization of each culture was required in order to combine compatible strains so that their usefulness in Mozzarella cheese manufacture would be maximized....
Resource Type:: Masters Thesis
Full Text:: Redacted for Privacy Abstract approved: Dr. William E. Sandine The present investigation was

Characterizing Mycobacterium avium subspecies hominissuis (MAH) Microaggregate Induction of Innate Immunity

Creator:: Keefe, Bailey F.
Abstract:: Bacterial aggregation is a strategy employed by many pathogens to establish infection. Mycobacterium avium subsp. hominissuis (MAH) undergoes a phenotypic change, microaggregation, when exposed to the respiratory epithelium. This aggregation is an important step in the pathogenesis of the infection, laying the foundation for biofilm formation. We therefore compared how...
Resource Type:: Masters Thesis
Full Text:: . Emerg Infect Dis 17:1760–1761. 9. Winthrop KL, McNelley E, Kendall B, Marshall-Olson A, Morris C

Effects of propionibacterial metabolites on spoilage and pathogenic bacteria in dairy products

Creator:: Teo, Alex Yeow-Lim
Resource Type:: Masters Thesis
Full Text:: Metabolites On Spoilage and Pathogenic Bacteria in Dairy Products Abstract approved by: William E. Sandine

Lactic streptococci: growth characteristics and plasmid profiles of drug- and bacteriophage-insensitive mutants

Creator:: Wulf, John J.
Abstract:: Fast acid-producing streptomycin-resistant mutants for several strains of lactic streptococci currently used in commercial Cheddar cheese manufacture were isolated. Their isolation involved use of a recently developed differential plating medium that allows identification of fast acid-producing colonies. Both parents and mutants were characterized with regard to growth, acid production, proteolytic...
Resource Type:: Masters Thesis
Full Text:: : Redacted for Privacy William E. Sandine Fast acid-producing streptomycin-resistant mutants for several

Studies on the inhibition of spoilage organisms in Cottage cheese by lactic streptococcus organisms

Creator:: Hauser, Bruce Allen
Abstract:: A report in the literature suggesting that the use of cottage cheese dressing cultured with S. diacetilactis would afford enhanced flavor and shelf life in the finished product prompted this study to examine reasons for its effectiveness. The method used involved addition of a culture of S. diacetilactis 18-16 to...
Resource Type:: Masters Thesis
Full Text:: Redacted for Privacy Abstract approved Dr. W. E. Sandine A report in the literature suggesting that

A taxonomic study on myxobacteria isolated from fish

Creator:: Porter, Sook Hee
Abstract:: While the genus Cytophaga has been defined and classified on a morphological basis, knowledge of fresh water members of this group of myxobacteria is scanty. In the present study cultural and morphological characteristics were investigated in an attempt to classify a group of fresh water cytophaga. Microscopic examination revealed all...
Resource Type:: Masters Thesis
Full Text:: Redacted for privacy Abstract approved: Dr. R. E. Pacha While the genus Cytophaga has been defined and

Isolation and characterization of a bacteriophage for Cytophaga succinicans (RL-8)

Creator:: Rust, Patricia Joy
Resource Type:: Masters Thesis
Full Text:: : fIlDr. R. E. Pacha Although bacteriophages have been recognized for a number of years, little

Characterization of microaggregate formation by Mycobacterium avium subspecies hominissuis

Creator:: Babrak, Lmar
Abstract:: Mycobacterium avium subsp hominissuis (MAH) is an opportunistic environmental pathogen that causes respiratory and gastrointestinal illness in immunocompromised persons such as those with chronic respiratory diseases or AIDs, respectively. In recent years, there has been a significant increase in the incidence of nontuberculous mycobacterial (NTM) lung infections, including in cystic...
Resource Type:: Dissertation
Full Text:: : ______________________________________________________ Luiz E. Bermudez Mycobacterium avium subsp hominissuis (MAH) is an opportunistic

Growth of lactococci relative to antibiotic and quaternary ammonium compounds

Creator:: Valladao, Marilin
Abstract:: The work presented in this thesis is concerned with the effect of several antibiotics and quaternary ammonium sanitizers upon growth of lactic acid bacteria. Section I reports the purification of beta-lactamase from Lactococcus cremoris PR-108, by ion exchange chromatography, using the chromogenic substrate pyridine-2-azo-p-dimethylaniline (PADAC) as the enzymatic indicator. Section...
Resource Type:: Masters Thesis
Full Text:: AMMONIUM CrOUNDS Redacted for PrivacyAbstract approved: William E. Sandine The work presented in this

Development of internally pH-controlled bulk starter media for the propagation of lactic acid bacteria

Creator:: Willrett, Douglas L.
Resource Type:: Dissertation
Full Text:: for privacy Abstract approved: William E. Sandine Various pH-controlled bulk starter media were

Identification and analysis of vaccinia virus acylproteins

Creator:: Hansen, Scott G.
Abstract:: Vaccinia virus (VV) encodes at least six proteins that are modified by the addition of a 14-carbon saturated fatty acid through an amide linkage and at least eight proteins that are modified post-translationally by the addition of a 16-carbon saturated fatty acid through linkage to cysteine residues. These post-translational modifications...
Resource Type:: Dissertation
Full Text:: of Vaccinia Virus Acylproteins Abstract approved: Dennis E. Hruby Vaccinia virus (VV) encodes at

Impacts of Environmental Change on a Newly-characterized Symbiosis between a Novel Bacterial Parasite, Genus Aquarickettsia, and the Coral Acropora cervicornis

Creator:: Klinges, J. Grace
Abstract:: Coral reef ecosystems continue to be significantly altered by disease epizootics, but why some host populations remain resistant while others succumb to outbreaks remains unknown. Research across diverse animal and plant host systems has revealed that disease severity is strongly influenced by host genetics and by environmental influences on both...
Resource Type:: Dissertation

Morphologic, molecular and antigenic characteristics of Bacteroides nodosus

Creator:: Gradin, Joseph Lloyd
Resource Type:: Dissertation
Full Text:: also extended to my committee members: Dr. John L. Fryer, Dr. Stephen L. Kaattari, Dr. Donald E

Survival and persistence of Bacteroidales human and ruminant specific fecal markers and occurrence with fecal pathogens

Creator:: Walters, Sarah P.
Abstract:: Aquatic fecal contamination from non-point sources impairs environmental health and serves as a vehicle for transmission of waterborne disease, resulting in economic losses worldwide. Accurate methods of diagnosing fecal pollution and its source are needed to prevent human exposure, remediate pollution, and reduce economic impacts. In order to obtain this...
Resource Type:: Dissertation
Full Text:: predictors of pathogen exposure, we evaluated co-occurrence of pathogenic E. coli O157:H7, Salmonella spp

Evaluation of peptide phosphorodiamidate morpholino oligomers as a novel antibiotic therapy

Creator:: Kaller, Margo
Abstract:: Peptide-phosphorodiamidate morpholino oligomers (PPMOs) are synthetic DNA mimics that bind and silence gene targets. Through designing PPMOs that silence essential or resistance genes in pathogens, these antisense oligomers could be utilized as novel antimicrobials. Towards this end, my thesis employed minimum inhibitory concentration assays (MICs) to identify PPMOs capable of...
Resource Type:: Masters Thesis
Full Text:: inhibitory concentration assays (MICs) to identify PPMOs capable of inhibiting in vitro growth of E. coli

Fecal Bacteroidetes host distributions and environmental source tracking

Creator:: Dick, Linda K.
Abstract:: Contamination of recreational and shellfish waters with fecal pollution is a major water quality issue with associated economic impacts and human health risks. Reliable fecal source identification and rapid, quantitative analyses are essential components of risk assessment. Enteric bacteria that are endemic to specific hosts have a potential role as...
Resource Type:: Dissertation
Full Text:: rapid than the E. coli assay. PCR source identification and enumeration of fecal Bacteroidetes 1 6S

Molecular characterization of crustacean parasite Nadelspora canceri

Creator:: Amogan, Harold
Abstract:: Investigations into the phylogeny, genome size, and karyotype of microsporidian Nadeispora canceri were initiated to further characterize the organism. Isolates of N. canceri spores were obtained from both Dungeness (Cancer magisrer) and red rock crabs (Cancer productus). Analysis of the ssu rDNA sequence from spore isolates of the two crab...
Resource Type:: Masters Thesis
Full Text:: would also like to thank Dr. Robert Olson for his help in specimen collection and the OSU Central

Arsenic methylation by marine cyanobacteria Prochlorococcus and Synechococcus

Creator:: Lee, Mikaela
Abstract:: Our experiments aimed to investigate responses of the marine cyanobacteria Prochlorococcus and Synechococcus to toxic arsenic species, such as arsenate, and to understand cellular mechanisms that contribute to arsenic tolerance. We used two strains of Prochlorococcus, a high-light adapted MED4 and low-light adapted MIT9313, both axenic, for cultivation experiments. For...
Resource Type:: Honors College Thesis
Full Text:: , Synechococcus, Arsenic, methylation Corresponding e-mail address: leemik@oregonstate.edu

Supplemental Description of Myxobolus squamalis (Myxozoa) & Epidemiology of M. squamalis at Two Oregon Hatcheries

Creator:: Polley, Tamsen M.
Abstract:: Myxobolus squamalis is a Myxozoan parasite of salmonids, which contributes to lowered fitness of hatchery and wild fish in the Pacific Northwest of North America. The only GenBank DNA sequence of M. squamalis is from Oncorhynchus tschawytscha (Chinook), but is not linked to a published morphological description. There is a...
Resource Type:: Honors College Thesis
Full Text:: . Corresponding e-mail address: polleyt@onid.orst.edu

Analysis of Bacteroidales 16S rRNA gene sequences from a geographically isolated human population

Creator:: Petersen, Carolyn A.
Abstract:: Standard methods of measuring fecal pollution in water do not distinguish between human and non-human sources. Molecular technology enabled the development of host-specific markers that distinguish fecal sources. Human specific PCR primers, HF183F and HF134F, were designed based on phylogenetic analyses of partial 16S rRNA gene sequences from the Bacteroidales...
Resource Type:: Masters Thesis
Full Text:: 13 2.6.1. Coliform-specific PCR... 14 2.6.2. E. coli toxin gene-specific PCR

Studies on glucose isomerase from Streptomyces flavogriseus

Creator:: Chen, Wen-Pin
Resource Type:: Dissertation
Full Text:: . Y. W. Han for many helpful suggestions and assist- ance. Dr. W. E. Sandine, Dr. M. W. Montgomery

Biophysical properties of the turnip yellow mosaic virus explored by coat protein mutagenesis

Creator:: Powell, Joshua D.
Abstract:: Plant viruses have been instrumental in our understanding of the biophysical properties pertaining to non-enveloped icosahedral virus particles. A substantial amount of research has been performed over five decades on Turnip yellow mosaic virus (TYMV), arguably one of the most extensively studied icosahedral plant viruses and the type-member of the...
Resource Type:: Dissertation
Full Text:: two mammalian viruses 31 Figure 1.7. The dual expression system for CP expression in E. coli

Bacterial 16S ribosomal DNA analysis of pyrrolizidine alkaloid detoxifying enrichments from the ovine rumen

Creator:: Gray, Diane R.
Abstract:: Bacterial cultures enriched from sheep rumen fluid have demonstrated the ability to detoxify pyrrolizidine alkaloids (seneciphylline and jacobine) in tansy ragwort (Senecio jacobaea). The microbes are difficult to isolate using classical anaerobic techniques, therefore, microbes from two different enrichment cultures demonstrating similar degradation activity were identified using their 16S ribosomal...
Resource Type:: Masters Thesis
Full Text:: chart of methods 42 2.3 Components of TYM and E media used to grow enrichment cultures 44 2.4

Evolution of a streamlined cooperator in Pseudomonas aeruginosa quorum sensing

Creator:: Huie, Jessica L.
Abstract:: Social behavior leading to the production of common goods is prone to exploitation. One such behavior in Pseudomonas aeruginosa is quorum sensing (QS), by which the bacteria produce signals to regulate extracellular common goods. Exploitation comes in the form of cheaters which have a mutation in the central quorum sensing...
Resource Type:: Masters Thesis
Full Text:: pJHlasR5, pJHpsdR1, and pJHabcB1. The plasmids were transformed into E. coli DH5α Max Efficiency cells

Characterization of Quorum-Sensing Diversity in Environmental Pseudomonas aeruginosa Populations

Creator:: Grellmann, Elise Kaylene
Abstract:: While the quorum-sensing (QS) diversity of the opportunistic pathogen Pseudomonas aeruginosa has been well established in cystic fibrosis (CF) lung infections and in vitro experiments, little is known about its QS-diversity in natural environments. One significant QS-deficient behavior is social cheating. Social cheaters contain mutations that prevent them from producing...
Resource Type:: Honors College Thesis
Full Text:: Corresponding e-mail address: grellmae@onid.orst.edu Copyright by Elise K

Study on the Evolution and Expression of the Pyoverdine Receptor FpvA inPseudomonas aeruginosa

Creator:: Singh, Amandip
Abstract:: Pseudomonas aeruginosa is a ubiquitous gram-negative bacterium that is capable of causing infections in people who are immune compromised. The bacterium can live in a variety of environments due to the variety of virulence factors it can produce. It is well adapted to living in iron-stringent conditions because it can...
Resource Type:: Honors College Thesis
Full Text:: , pyocin, evolution Corresponding e-mail address: singha@oregonstate.edu 2

Impacts of multispecies parasitism on coho salmon (Oncorhynchus kisutch) in Oregon

Creator:: Ferguson, Jayde A.
Abstract:: Diseases caused by parasites are recognized as significant sources of mortality in wild fish populations. I assessed the impacts of multispecies parasitism on a threatened stock of juvenile coho salmon (Oncorhynchus kisutch). A crucial prerequisite to this research was proper identification of parasites, which can be difficult for species lacking...
Resource Type:: Dissertation
Full Text:: . ................................................................................................... 157 Figure 5.2. Histological sections (H&E) of muscle parasites in coho salmon (Oncorhynchus

Quorum sensing gene regulation in Pseudomonas aeruginosa

Creator:: Gupta, Rashmi
Abstract:: Pseudomonas aeruginosa is an opportunistic human pathogen that infects immunocompromised individuals such as those suffering from burns or the genetic disorder cystic fibrosis. This organism utilizes a cell-cell communication mechanism known as quorum sensing (QS) to coordinate virulence gene expression and biofilm formation. It has three interconnected QS systems, namely...
Resource Type:: Dissertation
Full Text:: Effect of ectopic pqsA-E expression on colony morphology ............................83 3.9 Colony

DNA vaccines encoding the glycoprotein genes of spring viremia of carp virus, snakehead rhabdovirus, or infectious hematopoietic necrosis virus induce protective immunity in rainbow trout (Oncorhynchus mykiss) against an infectious hematopoietic necrosis virus lethal challenge

Creator:: Drennan, John D.
Abstract:: Recent advances in DNA vaccine technology has brought about a promising strategy for the control of viruses that contain surface membrane glycoproteins. This type of vaccine involves the intramuscular injection of a bacterial plasmid containing a gene encoding a viral protein. The strategy uses eukaryotic processing of the protein as...
Resource Type:: Masters Thesis
Full Text:: isotype switching. (Lobb & Olson, 1988; Killie et al., 1991) The absence of affinity maturation is

Isolation and characteristics of the DNA from nuclear polyhedrosis virus

Creator:: Carnegie, John William
Abstract:: The genome of the nuclear polyhedrosis virus which infects the larvae of the tussock moth, the lepidopteran, Hemerocampa pseudotsugata, was isolated, identified as deoxyribonucleic acid, and characterized in terms of its physical and biological properties. An isolation procedure for the viral DNA was established which consisted of the following steps:...
Resource Type:: Dissertation
Full Text:: Date thesis is presented /t//0? Typed by Donna L. Olson for John William Carnegie ACKNOWLEDGMENTS I

The effects of single and multiple pathogen and parasite infections on juvenile chinook and coho salmon during early marine residency

Creator:: Sandell, Todd A.
Abstract:: Nearly 3000 juvenile Chinook (Oncorhynchus tshawytscha) and coho (O. kisutch) salmon captured in nearshore waters off the coasts of Washington and Oregon from 1999-2004 were tested for infection by Renibacterium salmoninarum, Nanophyetus salmincola, and skin metacercariae. First, three quantitative PCR primer/probe sets were compared for detection of R. salmoninarum; amplification...
Resource Type:: Dissertation
Full Text:: phylogenetic study of the Digenea (Platyhelminthes: Trematoda) helped clarify the situation (Olson et al

Rapid, sensitive methods for enumeration and enterotoxin assay for Staphylococcus aureus

Creator:: Chugg, Lee Russon
Abstract:: A selective, differential plating medium for the enumeration of coagulase-positive staphylococci was developed. The ingredients of this medium were analysed for their effect on normal, heat treated and frozen staphylococcal cells. The nutrient base consisted of 10 gm Tryptone, 5 gm Beef Extract, 1 gm Yeast Extract and 17 gm...
Resource Type:: Dissertation
Full Text:: Graduate School Date thesis is presented / Typed by Donna L. Olson for Lee Russon Chugg

Studies on cellulase production from annual ryegrass straw by Trichoderma reesei

Creator:: Orberg, Paulo K.
Resource Type:: Masters Thesis
Full Text:: Dr. R. R. Becker and Dr. W. E. Sandine for kindly revising the manuscript, and to Dr. D. L. Willis

Establishment and characterization of three new cell lines from grass carp (Ctenopharyngodon idellus)

Creator:: Lu, Yuanan
Resource Type:: Masters Thesis
Full Text:: suggestions, and encouragement. His humor, criticism, and friendship were appreciated. To Dr. Robert Olson

Vitamin B₁₂ production and depletion in a naturally eutrophic lake

Creator:: Gillespie, P. A.
Abstract:: The vitamin B₁₂ concentrations in both the water and sediments of Klamath Lake were found to vary significantly during a one-year period. The patterns of B₁₂ distribution were remarkably similar among the three main sites. In all cases there was an extremely sharp gradation in concentration in relation to depth...
Resource Type:: Dissertation
Full Text:: Donna L. Olson for Paul Albert Gillespie ACKNOWLEDGMENTS This thesis has been made a reality through

Distribution, decay, and quantification of fecal source-tracking markers

Creator:: Green, Hyatt C.
Abstract:: Waterborne fecal contamination poses serious risks to human health and can disrupt aquatic ecosystems. Molecular marker methods are widely used to identify and, in some cases, quantify the sources of contamination and guide management decisions regarding water resource protection. However, methods to detect some likely sources of fecal contamination such...
Resource Type:: Dissertation
Full Text:: contaminant type where current monitoring methods (e.g., culturable E. coli and enterococci) fall short. The

Aspects of ergosterol biosynthesis in Saccharomyces cerevisiae

Creator:: Monner, David Anthony
Abstract:: The regulation of ergosterol biosynthesis in yeast has been studied. The system has been examined for the presence of an end-product inhibition. Both anaerobically and aerobically grown cells have been examined for this effect. A feedback inhibition of sterol synthesis has been shown, but it appears to be a secondary...
Resource Type:: Dissertation
Full Text:: under anaerobic conditions. At the same time Olson, et al. (1957) showed that molecular oxygen is

Culturing uncultured environmental microorganisms

Creator:: Connon, Stephanie A.
Abstract:: Research on natural environments, over the last decade, is replete with microbial diversity studies that used culture-independent approaches. The cloning and sequencing of the 16S rRNA genes has been the driving force in the expansion of awareness about the great diversity of previously undiscovered microorganisms. Well-known uncultured groups of microorganisms...
Resource Type:: Dissertation
Full Text:: , R. Goericke, R. J. Olson, B. Palenik, J. B. Waterbury, L. West-Johnsrud and E. R. Zettler (1992

Comparison of Renibacterium salmoninarum by antigenic analysis, antimicrobial assay and growth

Creator:: Getchell, Rodman Guy
Abstract:: The antigens of eight isolates of Renibacterium salmoninarum were compared serologically using immunoelectrophoretic techniques. Seven common antigens were identified by their electrophoretic mobilities in each antigenic profile. One of these common antigens, designated Fr 21-30, was partially purified by ammonium sulfate precipitation and gel filtration. Cross adsorption analysis showed Fr...
Resource Type:: Masters Thesis
Full Text:: to Dr. J. E. Sanders for reviewing this manuscript, Dr. J. Leong for her assistance, Rich Holt for

Studies on purification and characterization of an autolytic enzyme from cell walls of Bacillus subtilis

Creator:: Brown, Willie Claiborne
Abstract:: The purpose of this investigation was to purify and characterize the autolytic enzyme from cell walls of Bacillus subtilis 168. The crude enzyme was obtained by autolysis of purified cell walls in buffer at 37° C. Two purification methods were developed. The first involved fractional precipitation with ammonium sulfate. The...
Resource Type:: Dissertation
Full Text:: epartment of Microbiology Dean of Graduate School Date thesis is presented ; Typed by Donna Olson for

Effects of physiological state upon nitrosoguanidine-mediated allelic recombination in Saccharomyces cerevisiae

Creator:: Buettner, Michael John
Abstract:: Saccharomyces cerevisiae strain XS-380, a. heteroallelic diploid strain, carries six genetically analyzed loci on the left arm of chromosome VII. Nitrosoguanidine (NMG) at low concentrations was observed to cause allelic recombination (either mitotic recombination or gene conversion) of the heteroallelic loci with low lethality. The NMG-inducible heteroallelic system was used...
Resource Type:: Dissertation
Full Text:: different strains of E. coli, established the sequence and direction of replication by differing methods

Suppression of cellular immune responses by Candida albicans

Creator:: Rivas Espinoza, Victor G.
Abstract:: Experimental infection of mice with Candida albicans leads to significant suppression of cell mediated immune responses. We have characterized the nature of this candida-induced suppression with formalin killed Candida albicans (FKCA) in the yeast and mycelia' phase. Mice (BALB/c or C57B1/6J) treated with FKCA exhibited a reduction in the T-cell...
Resource Type:: Masters Thesis
Full Text:: manuscript. The Universidad Veracruzana and Asociacion Nacional de Universidades e Institutos de Enzeflanza

Immune responses of juvenile chinook salmon (Oncorhynchus tshawytscha) to p,p-̓DDE and tributyltin

Creator:: Misumi, Ichiro
Abstract:: In this thesis, we examined the effects of the exposures to anthropogenic pollutants on the fish, primarily juvenile chinook salmon, immune system using newly and recently developed immune assays. In addition, we developed a new assay for measuring immunocompetence of fish. In the first chapter, the Alamar Blue assay was...
Resource Type:: Masters Thesis
Full Text:: and Kruskal-Wallace test). 2.6 2.4 a) 0 2.O 1.8 C', E 1.6 1.4 1.2 1.0 2.4 2.2 >< a) -o

Quantifying Carbon Production in Marine Ecosystems Using Expressions of Phytoplankton Physiology

Creator:: Penta, William Bryce
Abstract:: Phytoplankton initiate the marine carbon cycle by fixing carbon dioxide into biologically available compounds. These abundant single celled organisms mediate carbon flux from the atmosphere to the deep ocean and are the base of the marine food web, supplying new carbon to higher trophic levels. Phytoplankton are highly diverse and...
Resource Type:: Dissertation

Hematology and histopathology of Coho salmon (Oncorhynchus kisutch) infected with Flexibacter psychrophilus

Creator:: Kanchanakhan, Somkiat
Resource Type:: Masters Thesis
Full Text:: ProfessoKand Chairm the Department of Microbiology in charge of major Redacted for Privacy Dean of the Grad e

Nucleic acids associated with MC29 tumor virus

Creator:: Weber, George Harding
Resource Type:: Dissertation
Full Text:: School Date thesis is presented nti ,16)f)1 Typed by Cheryl E. Curb for George Harding Weber

Analysis of segregants of F'gal heterogenotes in Escherichia coli

Creator:: Kurahara, Carole Mahaney
Abstract:: The purpose of this study was to isolate and characterize any segregants of the heterogenotes formed from sexduction by an F' donor. Following the mating of F' W4520, carrying an F' gal₁⁺ gal₂⁺ factor, with W3350, a gal₁⁻ gal₂⁻ recipient, a number of recombinant clones were picked and examined for...
Resource Type:: Masters Thesis
Full Text:: , 1967 Typed by Donna Olson for Carole Mahaney Kurahara ACKNOWLEDGMENTS What we easily learn, we

Anaerobic biodegradation of the cyclic nitramines, RDX and HMX, by ovine ruminal microbes

Creator:: Eaton, Hillary L.
Abstract:: Cyclic nitramines released into all environmental compartments through anthropogenic activities are toxic and possibly carcinogenic and mutagenic. Soils on military ranges, located throughout the world in various climatic regions and close to human activities, are especially susceptible to cyclic nitramine contamination. The properties of soils on military ranges will directly...
Resource Type:: Dissertation
Full Text:: quadrupole mass spectrometry LOD limit of detection LNB low nitrogen basal medium ME medium E

The combined effect of herpes simplex virus type 2 and a polychlorinated biphenyl (Arochlor 1242) on human chromosomes : induction of sister chromatid exchanges

Creator:: Nicholas, Ann Harriet
Resource Type:: Dissertation
Full Text:: especially grateful to Dr. Herman E. Wyandt, Division of Medical Genetics, University of Oregon Health

Interactions between microbial dynamics, water flow, and solute transport in unsaturated porous media

Creator:: Yarwood, Rockie R.
Abstract:: Bioremediation in the vadose zone is unpredictable because of poor understanding of factors influencing microbial growth in this environment. A lab-scale experimental system was developed to examine, noninvasively, interactions between microbial growth, water flow, and solute transport in unsaturated porous media. Measurements of microbial colonization, and its impact on hydrology,...
Resource Type:: Dissertation
Full Text:: , germicidal lamp; B, influent drip emitters; C, siphon tube; D, glass sheets; E, spacer; F, 0-ring seal; G

Pasteurella multocida infection in turkeys : pathogenesis and immunity

Creator:: Tsuji, Masayoshi
Abstract:: Pathogenesis and immunity involved in fowl cholera were studied with the turkey, one of the most susceptible natural hosts. The causative agent, Pasteurella multocida, was analyzed in terms of its virulence and immunogenic factors, using a highly virulent, encapsulated strain P-1059. Protective immunity was induced by lipopolysaccharide (LPS)- protein complex...
Resource Type:: Dissertation
Full Text:: the somatic antigens. Employing these methods, five capsular types (A, B, D, E, and F) and 16 somatic

Stable isotope probing of the ovine rumen for RDX degrading microorganisms

Creator:: Mitchell, Edward A.
Abstract:: The anaerobic and highly reductive conditions found in the ovine rumen are considered favorable for the degradation of the nitroaromatic explosives such as hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). In this study, we used stable-isotope-probing to determine the bacteria responsible for the degradation of RDX in the rumen. Results indicate 10 μg mL⁻¹ (45...
Resource Type:: Masters Thesis
Full Text:: differentiate the labeled and unlabelled DNA. To obtain 13C-DNA, E. coli DH5α was grown on uniformly labeled

A bacterial disease of the American shad (Alosa sapidissima)

Creator:: Rothman, Stanley
Abstract:: For the past six years a bacterial infection has been the cause of large losses of adult, spawning, American shad (Alosa sapidissima) in the Coos, Millicoma and Smith Rivers of Oregon. There was a sizable commercial fishery for shad in these rivers and losses of fish due to this infection...
Resource Type:: Masters Thesis
Full Text:: Olson for Stanley Rothman Redacted for Privacy Redacted for Privacy Redacted for Privacy

Microbiology of basalts targeted for deep geological carbon sequestration : field observations and laboratory experiments

Creator:: Lavalleur, Heather J.
Abstract:: With rising concentrations of CO₂ in the Earth's atmosphere causing concern about climate change, many solutions are being presented to decrease emissions. One of the proposed solutions is to sequester excess CO₂ in geological formations such as basalt. The deep subsurface is known to harbor much of the microbial biomass...
Resource Type:: Masters Thesis
Full Text:: : A Handbook of Physical Restraints, American Geophysical Union. Boyd E, Cummings D, Geesey G

Development of monoclonal antibodies against the myxosporean, ceratomyxa shasta, and characterization of the salmonid host response

Creator:: Bartholomew, Jerri Lee
Abstract:: Monoclonal antibodies (Mabs) were produced against the myxosporean parasite, Ceratomyxa shasta, and were characterized by indirect immunofluorescent techniques, western blot analysis, and immunoelectron microscopy. The resulting Mabs reacted specifically with the trophozoite and sporoblast stages of the parasite, but not with the mature spore, indicating that certain antigens are stage-specific....
Resource Type:: Dissertation
Full Text:: LaPatra, Tony Amandi, Rich Holt, John Kaufman, Cathy Lannan, Bob Olson, Leslie Smith, Harriet Lorz and

Growth rate effects during starvation-survival of a marine psychrophilic vibrio

Creator:: Moyer, Craig Lee
Abstract:: Cell populations of the marine bacterium ANT-300, from either batch culture or continuous culture with dilution rates ranging from D = 0.170 h⁻¹ (fast) to D = 0.015 h⁻¹ (slow) were monitored for 98 days under starvation. Viability (CFU), acridine orange direct counts (AODC), and optical density were measured. DNA,...
Resource Type:: Masters Thesis
Full Text:: population; the vertical bars represent the standard error of the mean. 7.00 6.00 ro E 5.00 `--1 4.00 D

High resolution digital imaging of bacterial cells

Creator:: Siebold, William A.
Abstract:: The most abundant clone found in ribosomal RNA clone libraries obtained from the world's oceans belongs to the SAR11 phylogenetic group of environmental marine bacteria. Imaging and counting SAR11 bacterial cells in situ has been an important research objective for the past decade. This objective has been especially challenging due...
Resource Type:: Masters Thesis
Full Text:: random target 30.0 V 0 E 20.0 0 0a 0 0.0 0 four million base-p aIr genome - one million base-pair

Construction of a model organism for performing calcium carbonate precipitation in a porous media reactor

Creator:: Kaufman, Megan J.
Abstract:: Aquifers are an important storage location and source of fresh groundwater. They may become polluted by a number of contaminants including mobile divalent radionuclides such as strontium-90 which is a byproduct of uranium fission. A method for remediating such divalent radionuclides is sequestration through co-precipitation into calcium carbonate. Calcium carbonate...
Resource Type:: Masters Thesis
Full Text:: E. coliDH5α(pURE14.8) were as follows:…………………………………………………………………... 99 LIST

Characterization of a rhabdovirus isolated from the snakehead fish (Ophicephalus striatus)

Creator:: Kasornchandra, Jiraporn
Resource Type:: Dissertation
Full Text:: , Leslie Smith, Craig Banner, Dr. Jim Bertolini, Dr. Bob Olson, Dr. Paul Reno, and Prudy-Caswell Reno, for

Methionine recycling in fruit studies on the metabolism and function of selected intermediates

Creator:: Gander, Lise Marie K.
Abstract:: This research has focussed upon both the in vitro and in vivo characterization of the recycling pathway leading from 5'-deoxy-5'-methylthioadenosine to methionine in fruit. In addition to contributing towards polyamine biosynthesis and other important cellular functions, in plant tissues these events also serve to generate the hormone ethylene. A broad...
Resource Type:: Masters Thesis
Full Text:: ). The effect also has been observed in LDC mutants of E. coli (49), suggesting two general paths for

In vitro regulation and cultivation of salmonid lymphocytes

Creator:: DeKoning-Loo, Jenefer
Abstract:: The foundation of the research compilation presented here began with the derivation of an improved method for obtaining optimal in vitro mitogenic responses in salmonid lymphocyte cultures by utilizing autologous or homologous plasma as the primary serum supplement. It was observed that lymphocytes which were previously unresponsive to mitogenic challenge...
Resource Type:: Dissertation

Effects of Anthropogenic Stressors on Seagrass and Coral Microbiomes

Creator:: Wang, Lu
Abstract:: Seagrasses and coral reefs play important roles in nutrient cycling, coastal protection, and maintaining marine biodiversity. However, these coastal marine organisms are declining globally due to anthropogenic stressors, such as rising ocean temperatures, ocean acidification, and eutrophication. These organisms live in close association with their microbiomes, which can be beneficial...
Resource Type:: Dissertation
Full Text:: preparation Deron E. Burkepile: Study design and conception, fieldwork, data analysis, manuscript edits

The exploitation of methylthioadenosine phosphorylase deficiency in cancer therapy

Creator:: Lindburg, Katherine L.
Abstract:: Methylthioadenosine (MTA) is a naturally occuring nucleoside formed during the synthesis of polyamines in mammalian cells. MTA is rapidly catabolised by MTA phosphorylase (MTAase), eventually yielding methionine and adenine. MTAase has been shown to be active in all normal cells, but deficient in some cells and tissues of malignant origin....
Resource Type:: Masters Thesis

The Fitness Costs and Regulatory Strategies of Cooperative Siderophore Production in Pseudomonas

Creator:: Sexton, David
Abstract:: Cooperative behaviors in bacteria are increasingly appreciated for their relevance to microbial ecology and utility as model systems for social evolution. One example is the secretion of siderophores, a structurally diverse group of compounds that chelate extracellular iron. Siderophore production is considered cooperative because the benefits can be shared with...
Resource Type:: Dissertation

Microbial diversity, metabolic potential, and transcriptional activity along the inner continental shelf of the Northeast Pacific Ocean

Creator:: Bertagnolli, Anthony D.
Abstract:: Continental shelves located along eastern boundary currents occupy relatively small volumes of the world’s oceans, yet are responsible for a large proportion of global primary production. The Oregon coast is among these ecosystems. Recent analyses of dissolved oxygen at shallow depths in the water column has suggested increasing episodes of...
Resource Type:: Dissertation

Characterization of an inhibitor ("6S") of infectious pancreatic necrosis virus (IPNV) in normal rainbow trout serum (RTS) and its effects on the virus

Creator:: Park, Kyoung Chul
Abstract:: The characteristics of an inhibitor of infectious pancreatic necrosis virus (IPNV) found in normal rainbow trout serum (RTS) were studied. The serum inhibitor had a molecular weight of approximately 150 kDa and was dependent on divalent cations, either Ca²⁺ or Mg²⁺. It was stable at temperatures up to 50°C and...
Resource Type:: Dissertation
Full Text:: . Anthony Vella, Dr. Daniel Rockey, and Dr. Mark Christensen for their support. I also thank Dr. Bob Olson

Strain differentiation and detection of infectious hematopoietic necrosis virus

Creator:: LaPatra, Scott Edward
Abstract:: The virulence of selected isolants of infectious hematopoietic necrosis virus (IHNV) were differentiated and correlated with electrophoretic mobilities of virion structural proteins by sodium dodecylsulfate polyacrylamide gel electrophoresis. A type 1 electropherotype of IHNV was more virulent for kokanee salmon (Oncorhynchus nerka) and a type 2 strain more virulent for...
Resource Type:: Dissertation
Full Text:: Lannan, Bob Olson, Jim Winton and Chris Dunagan. My deepest thanks to my mother Jean Rowe and Preston

The role of costimulation and adjuvants in the development of T cell effector and memory responses

Creator:: Maxwell, Joseph R.
Abstract:: T cells are one of the key cells in the immune system. Although they are not the first line of defense against a pathogen, their functions can greatly enhance the phagocytosis and destruction of pathogens as well as the development of antibody responses. Furthermore, even when responding T cells have...
Resource Type:: Dissertation

Impacts of microbial chemical interactions on marine plankton physiology

Creator:: Moore, Eric R.
Abstract:: The bioavailability of chemical compounds in the marine environment fundamentally influences the growth and physiology of microorganisms. Organic and inorganic chemicals that are produced by some marine plankton can be consumed by other plankton for energy production, growth, or to initiate essential physiological processes. Cultures of the diatom Thalassiosira pseudonana,...
Resource Type:: Dissertation
Full Text:: . This interaction 465 established a positive feedback loop where E. huxlei stimulated Roseobacter

What is killing the corals? Viral and bacterial interrogations using metagenomics and microscopy

Creator:: Soffer, Nitzan
Abstract:: Corals have multiple roles in maintaining ocean health and are some of the world’s most diverse ecosystems. The coral animal is host to a multitude of taxa, including symbiotic dinoflagellate algae, fungi, bacteria, protists, and viruses. Environmental stressors and disease agents can perturb the delicate balance of the coral host...
Resource Type:: Dissertation
Full Text:: POTENTIAL ROLE OF VIRUSES IN WHITE PLAGUE CORAL DISEASE Nitzan Soffer, Marilyn E. Brandt, Adrienne

Development of immunological memory in rainbow trout (Oncorhynchus mykiss) and aflatoxin B₁ modulation of the response

Creator:: Arkoosh, Mary
Abstract:: Immunological memory has been shown to occur unequivocally in mammals. However, controversy exists regarding the capability of fish to elicite a memory response. This study demonstrates that memory does occur rainbow trout, Oncorhynchus rnykiss. Trout injected with a T-dependent antigen, trinitrophenylated keyhole-limpet hemocyanin, (TNP-KLH) (100 μg/fish) were able to produce...
Resource Type:: Dissertation
Full Text:: response of IgG memory cells induced by TNP-KLH (T-dependent) and TNP-T4 (E. coli phage T4) (T

Harnessing Heterogeneity in Human Gut Microbiome Data

Creator:: Tataru, Christine
Abstract:: The focus of this thesis is to design, characterize, and apply novel computational methods and molecular systems to interrogate heterogeneous human gut microbiome-related phenomena. In Chapter 2, I design, implement, and characterize a method for embedding co-occurrence patterns derived from massive 16s amplicon datasets. I use this method to 1....
Resource Type:: Dissertation
Full Text:: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279 D Chapter 5 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 290 E Chapter 6

Developmental of a novel affinity ELISA and ita application to the analysis of affinity maturation in trout

Creator:: Shapiro, David A.
Resource Type:: Dissertation
Full Text:: only one nitro group. Lysine e-amino group participation in protein conjugation was suggested by

Comparative genomics of Vibrio and characterizing microbial communities at a shellfish hatchery

Creator:: Delgado, Hanna
Abstract:: Vibrio bacteria are commonly found in freshwater, marine, and estuarine environments. Members of this genus can exist in commensal relationships with marine animals or as planktonic bacteria, however, they are well known for their roles as pathogens towards humans and animals. In this dissertation, I investigated aspects of host-bacteria relationships...
Resource Type:: Dissertation
Full Text:: –315. https://doi.org/10.1099/ijs.0.02402-0 Ben-Haim, Y., Rosenberg, E., 2002. A novel Vibrio sp

Single-Cell Genomics of Uncultivated Marine Bacteria

Creator:: Landry, Zachary
Abstract:: Historically, the difficulty of obtaining pure cultures of abundant marine microbial plankton has an obstacle to reconstructing the underlying mechanisms of biogeochemistry in the ocean. While a number of dominant marine species from the ocean surface have been cultured, the dominant microbial plankton of the dark ocean proved far more...
Resource Type:: Dissertation

Cooperation and conflict in Pseudomonas aeruginosa quorum sensing

Creator:: Wilder, Cara N.
Abstract:: Pseudomonas aeruginosa is an environmental bacterium as well as an opportunistic pathogen that primarily infects immunocompromised individuals, including those suffering from cystic fibrosis. The density-dependent regulation of gene expression via cell-to-cell communication, also termed quorum sensing (QS), is an important virulence determinant in this organism. Generally, P. aeruginosa uses three...
Resource Type:: Dissertation

Search Constraints

Search Results

Limit your search