This project was initiated in an attempt to control furunculosis
(Aeromonas salmonicida) in a production hatchery rearing coho salmon.
An oral vaccine was prepared and administered to 520,000
juvenile coho salmon (Oncorhynchus kisutch) at the Siletz River Salmon
Hatchery prior to the onset of a predictable naturally occurring
epizootic of...
This project was initiated to determine the incidence, distribution
and species of fish affected by Ceratomyxa shasta in Oregon
waters. Returning adult Pacific salmon and steelhead trout were
chosen for the survey because they were known to be susceptible to
infection. The parasite was also believed to be an important...
The purpose of this project was to study the possibility of controlling
furunculosis in salmonid fish using immunological procedures.
Antiserum with an agglutinating antibody titer of 1:20,480
against Aeromonas salmonicida was produced in a horse. This
hyperimmune serum was used to passively immunize coho salmon,
Oncorhynchus kitsutch, (jacks). However, this...
This investigation was initiated in an attempt to delineate
some factors affecting the adsorption of poliovirus to soil particles
in seawater. The results could have significance in the development
of assay procedures for viral contaminants in natural waters.
A sample of marine sediment, obtained from an estuary near
Purdy, Washington,...
The purpose of this paper was twofold, (1) to determine the
distribution of the infective stage of Ceratomyxa shasta (Noble) in the
Deschutes River basin, and (2) to study the ultrastructure of the spore
stage of the protozoan parasite.
Liveboxes containing juvenile rainbow trout were placed at
specific test points...
After parenteral immunization with Aeromonas salmonicida
cells, the agglutination activity in juvenile coho salmon (Oncorhynchus
kisutch) serum was associated with the macroglobulin serum fraction.
Antibody activity was diminished by 30 minutes exposure to 50 C
but not 45 C. Reduction with 2-mercaptoethanol and alkylation
markedly lowered but did not completely...
The detection and antigenic nature of the causative Corynebacterium
of bacterial kidney disease and chemotherapy of this disease
in juvenile coho salmon (Oncorhynchus kisutch) were examined.
Each of 207 yearling coho salmon collected from a population
undergoing a severe epizootic of bacterial kidney disease were examined
for the presence of...
An oral vaccine was developed for control of vibriosis
in Pacific salmon. The vaccine consists of a lyophilized
sonicate of cells of Vibrio anguillarum, the causative
agent of this disease. The preparation was incorporated
into a ration and fed to salmon in fresh water before the
fish were transported and...
This research was initiated to investigate the effects of various
parameters on the immune response of juvenile coho salmon to Aeromonas
salmonicida. The antibody response and development of protective
immunity in juvenile coho salmon were followed after a single
intraperitoneal injection of formalin killed A. salmonicida cells emulsified
in Freund's...
The Boivin agent from Vibrio anguillarum was extracted with the use of 0.25M trichloroacetic acid (TCA). Chemical analysis revealed that the endotoxin contained approximately 35.1% protein, 18.1% carbohydrate, 39.3% lipid, 1.8% glucosamine and evidence of ethanolamine. Sixteen different amino acids made up the protein. The aliphatic amino acid concentration was...
Juvenile coho salmon (Oncorhynchus kisutch) were orally
immunized against the bacterium Flexibacter columnaris (formerly
Chondrococcus columnaris), the causative agent of columnaris disease
in fish, with a vaccine-food preparation containing formalin-killed
cells. Fish immunized for 1, 2, 3, and 4 months were challenged
with selected concentrations of F. columnaris by exposure...
The purpose of this study was to find what effect temperature
and immunization has on the phagocytic activity of fish leukocytes
against certain disease causing bacteria. Also proposed was the
isolation and characterization of a leukocytolytic factor produced by
one of these bacteria, Aeromonas salmonicida.
Investigations revealed that temperature had...
Efficacious vaccines were developed for the control of Vibrio
anguillarum, the etiological agent of vibriosis in salmonid fish.
These bacterins can be administered either orally or parenterally.
It was determined that both formalin-killed lyophilized whole cells
and wet-packed whole cells of the organism are effective oral immunogens.
Intraperitoneal injection of...
Phenol-water and trichloroacetic acid extracts of Vibrio
anguillarum were both antigenic and immunogenic when injected
into coho salmon (Oncorhynchus kisutch). Cross-protection against
virulent Vibrio anguillarum was observed with whole-cell bacterins
and phenol-water extracts prepared from two serologically distinct
groups. Gel diffusion studies indicated that intact somatic antigens
were serotype specific....
An additional layer, exterior to the outer membrane cellwall
layer, was found on Aeromonas salmonicida cells from aggregating
strains. Nonaggregating strains were found to lack this layer.
The nonaggregating strains were all avirulent (LD₅₀
greater than
1 X 10
⁸ CFU/fish) while the majority of the aggregating strains
were virulent...
The distribution in Oregon of two viruses affecting salmonid
fishes was determined by examination of hatchery and feral fish.
Infectious pancreatic necrosis virus (IPNV) was isolated from fish
at the Oregon Department of Fish and Wildlife Research Laboratory,
Corvallis, Roaring River, Fort Klamath, and Wizard Falls Hatcheries.
Fish collected at...
The occurrence and distribution of fish viruses in Oregon were
determined by the examination of anadromous and fresh water salmonids.
Infectious pancreatic necrosis virus (IPNV) was isolated and
identified from asymptomatic adult coho (Oncorhynchus kisutch) and
chinook (0. tshawytscha) salmon returning to the Columbia River
system. Infectious pancreatic necrosis virus...
Unfertilized ova from spring chinook salmon (Oncorhynchus
tshawytscha) were examined for the presence of classical salmonid
inmmunoglobulin. Immunodiffusion techniques in which rabbit anti-salmonid
immunoglobulin was reacted against an ova homogenate
and rabbit anti-ova homogenate was reacted against immune chinook
serum failed to detect classical-type antibodies within these eggs.
A lectin...
This study was undertaken to compile a comprehensive
review of the literature and to gain a better understanding
of the host-parasite relationship in corynebacterial kidney
disease of salmonid fishes. An indirect fluorescent antibody
technique was developed which allowed differentiation
of intra- and extracellular bacteria. This technique was
used in combination...
Persistent infections with infectious pancreatic necrosis virus
(IPNV) were established in two salmonid cell lines and in live brook
trout. Virus inocula containing high concentrations of defective
interfering IPNV enhanced but were not a requirement for the establishment
of persistent infections in cell lines derived from steelhead
trout (STE-137) and...
Experiments were designed to more precisely evaluate the effects of water temperature on the progress of bacterial kidney disease ( BKD) in three species of salmonid fishes. Infections were produced by intraperitoneal injection of suspensions of the causative organism into fish held at seven temperatures ranging from 3.9 to 20.5°C....
Fish, asymptomatically infected with Enteric Redmouth
Bacterium (ERMB), were tested for their ability to transmit
bacteria to non-infected fish. Unstressed carrier fish
were unable to transmit enough bacteria to cause
epizootics or produce carrier fish. However, when the
ERMB carriers were stressed with heat, sufficient bacteria
were shed to infect...
Six salmonid cell lines previously established in this laboratory,
CHSE 114 and CHSE 214 (chinook salmon), CSE 119 (coho salmon),
SSE 5 (sockeye salmon), STE 137 (steelhead trout) and RTH 149 (rainbow
trout), were partially characterized. Mycoplasmas were not
detected in any of the cell lines.
All cell lines exhibited...
Immunity in juvenile coho salmon (Oncorhynchus kisutch) was
evaluated after immunization against vibriosis by parenteral, oral or
immersion methods. Relative levels of immunity were determined by
protection of fish from experimental water-borne challenges with
Vibrio anguillarum and by serum agglutination titers. Coho salmon
eggs were exposed to bacterin during artificial...
The antigens of eight isolates of Renibacterium
salmoninarum were compared serologically using
immunoelectrophoretic techniques. Seven common antigens
were identified by their electrophoretic mobilities in each
antigenic profile. One of these common antigens, designated
Fr 21-30, was partially purified by ammonium sulfate
precipitation and gel filtration. Cross adsorption analysis
showed Fr...
Twenty-two isolates of Flexibacter columnaris were
collected from different species of fish from a wide
geographic range. Shieh medium was selected and modified
to provide satisfactory growth of F. columnaris strains.
Although there were three phenotypic variations in colony
morphology among the strains observed, the environmental
and biochemical characteristics as...
This report describes the isolation of a new virus from adult
chum salmon (Oncorhynchus keta) returning to the Tokushibetsu Hatchery,
Hokkaido, Japan. The agent was isolated in the CHSE-214 cell line
derived from chinook salmon (Oncorhynchus tshawytscha). The virus
replicated in selected fish cell lines incubated between 10 and 20°C...
Fish diseases and various parameters associated with disease
caused mortality of fish were monitored at the Oregon State University
Marine Science Center and at a private mariculture facility on
Yaquina Bay during a period of five years. Nearly all disease problems
observed were caused by Vibrio anguillarum and Vibrio spp....
A comparison of the agglutinating and precipatiating
antigens of Enteric Redmouth Bacterium (ERMB) was made.
There are two major and one minor agglutinating antigens
which describe two serotypes (I and II). Only serotype I
metabolizes sorbitol. A bacterin from serotype I cross
protected against a challenge of bacteria of serotype...
The purpose of this study was to characterize Cytophaga
psychrophila isolates obtained from coho fingerlings with low temperature
disease collected at selected hatcheries in Oregon. In
addition, cultures thought to be related to this bacterium were
isolated from Siletz Hatchery water, deformed juvenile and spawning
adult coho salmon, rainbow and...
Morphological, cultural, biochemical and serological characteristics of
33 bacterial strains, thought to be similar to Cytophaga psychrophila, the
causative agent of bacterial cold-water disease were compared. Bacterial
strains identified as C. psychrophila were obtained from diseased salmonids
collected at widely separated geographic locations, and from fish with
pathological signs different...
Vaccination with Vibrio anguillarum by oral administration
during copper exposure and intraperitoneal injection prior to copper
exposure was employed to investigate the effects of copper upon survival
and the immune response of juvenile coho salmon (Oncorhynchus
kisutch). Following copper exposure the survivors were challenged
under natural conditions to V. anguillarum,...
Oregon Department of Fish and Wildlife trout broodstocks from Alsea, Cole Rivers, Klamath, Leaburg, Oak Springs, Roaring River and Willamette Fish Hatcheries were examined for the presence of myxosporidans. Wild fish collected from waters above these hatcheries were also examined. Seven species of myxosporidans were observed in the hatchery broodfish:...
The virulence of selected isolants of infectious hematopoietic
necrosis virus (IHNV) were differentiated and correlated with
electrophoretic mobilities of virion structural proteins by sodium
dodecylsulfate polyacrylamide gel electrophoresis. A type 1
electropherotype of IHNV was more virulent for kokanee salmon
(Oncorhynchus nerka) and a type 2 strain more virulent for...
A new Lactobacillus species is proposed to include 17 strains of a bacterium from rainbow (Salmo gairdneri) and cutthroat trout (Salmo clarki), and chinook salmon (Oncorhynchus tshawytscha). The bacterium was isolated most frequently from fish stressed by handling or spawn ing. Phenotypically, the strains belong to the family Lactobacillaceae and...
Monoclonal antibodies (Mabs) were produced against the
myxosporean parasite, Ceratomyxa shasta, and were characterized
by indirect immunofluorescent techniques, western blot analysis,
and immunoelectron microscopy. The resulting Mabs reacted
specifically with the trophozoite and sporoblast stages of the
parasite, but not with the mature spore, indicating that certain
antigens are stage-specific....
For the past six years a bacterial infection has been the cause
of large losses of adult, spawning, American shad (Alosa
sapidissima) in the Coos, Millicoma and Smith Rivers of Oregon.
There was a sizable commercial fishery for shad in these rivers
and losses of fish due to this infection...
The ability of Flexibacter columnaris to attach to fish
cells was studied. Four of the five bacterial strains
tested were able to adhere to fish cells derived from four
different fish species. The attachment of these strains
in vitro required magnesium but was not affected by
increasing the incubation temperature...
Four surface and three subsurface horizons of agriculturally
important Oregon soils were exposed to excessive volumes of anhydrous
ammonia and reequilibrated with the atmosphere for several
time periods prior to extraction with water. The carbon solubilized
was determined by a modified wet combustion technique. Nitrogen
in the soils, extracts, and...
This programmed text covers the basic elements of the theory
of modulation. It is by no means a comprehensive reference to
modulation topics. The text is designed to introduce the student to
modulation in general terms. After successful completion of this
text, the student should be able to advance to...
In communications it is often desirable to recover a signal
hidden in noise. The techniques of correlation are often employed
for this purpose. Initially, the basic formula and properties of correlation
including an approximating formula are presented. Then the
description, design, and construction of a DC to 500 KHz electronic...
The effects of soil temperature and soil water suction on the
uptake of calcium, magnesium, and potassium by wheat (Triticum
aestivum VILL., Host) were studied. Companion studies on uptake of
nitrogen and phosphorus provided background for discussion of the
results. Plants were grown in perlite slabs separated from an
osmotic...
When selecting an approximate integration technique for use in
a digital differential analyzer, such factors as cost, parts count,
power dissipation and solution accuracy must be considered. This
paper considers the application of the classical rectangular and
trapezoidal integration techniques and a new two cycle method of
integration, all implemented...
To understand the processes that lead to the formation of the oceanic crust, one must know the composition and the depth at which primary melts originate. Towards this end, this dissertation focuses on plagioclase-hosted melt inclusions from plagioclase ultraphyric basalts (PUBs). Plagioclase is usually considered to be the second phase,...
This paper presents an application of the wave equation to the
problem of selecting a driving hammer for prestressed concrete piles.
The potentially damaging effects of these types of hammers on various
prestressed concrete piles were analyzed by means of parametric
studies carried out on a hypothetical group of problems...
A greenhouse experiment was conducted in 1991 and in
1992 to determine the effect of soil water pressure on
potato (cv Russet Burbank) plant growth and infection by
Verticillium dahliae. Soil water pressures of -0.01,
-0.03, -0.08, or -0.15 MPa were combined factorially with
two inoculum concentrations, 0 or 10...
Life-history organization of the cutthroat trout (Oncorhvnchus clarki) may be viewed at various levels, including species, subspecies, metapopulation, population, or individual. Each level varies in spatial scale and temporal persistence, and components at each level continually change with changes in environment. Cutthroat trout are widely distributed throughout the western USA,...
The principal aim of this study was the use and evaluation of dynamic
modeling techniques to identify mathematical models for cereal drying rates and
quality changes that best describe thin-layer malt drying data. Seven thin-layer
malt drying experiments were performed at the Great Western Malting pilot
facilities, Vancouver, WA. Malt...
Heartwood can have properties that are distinct from sapwood,
including resistance to insect and microbial attack. Despite the practical
importance of heartwood formation in trees, a review of the literature revealed
that little was known about the effects of environmental factors on heartwood
quality or how variations in heartwood properties...
This study examined the decay resistance of the pine and maple components of wood plastic composites (WPC' s) of varying thicknesses exposed on several culture media to wood decay fungi under laboratory conditions. The ability of malt agar extract (MEA), potato dextrose agar (PDA), amended basal salts, sawdust (maple and...
A new carbohydrate-phenol based resorcinol resin
(CPR resin) was synthesized by end capping a known
carbohydrate-phenol based resole with resorcinol under
basic reaction conditions. Synthetic parameters, such as
duration of cook, pH, and the amount of resorcinol and
formaldehyde were varied to produce resins with molecular
weights (Mw) ranging from...
The widespread introduction of trout to naturally fishless mountain lakes in the western United States has been accompanied by little research. The ecological role of trout populations occurring in 91 lakes of the central Selway Bitterroot Wilderness, Idaho, was examined with respect to 1) the sampling variability of biological and...
Disturbances are a prevalent and important part of ecosystems. Many landscape patterns that we find today were created, maintained, and changed by natural disturbance regimes. This is especially true for fire, which has historically been a common, natural disturbance in the western U.S forests and grasslands and many other parts...
The primary goal of this study is to assess the impact of a subduction component
added to the mantle wedge beneath the Oregon Cascades to the composition and fO2 of
primitive Cascade basalts. Olivine-hosted melt inclusions from compositionally diverse
basalts across the Cascade arc (~100 km) are utilized in an...
A simple heat exchanger network has been studied as
an example of a dynamically interactive process. The
process is relatively simple, requiring only temperature
measurements and flow rate manipulations. Equations are
presented for the design of a physical process having a
specified steady-state Relative Gain Array and specified
dynamic characteristics....