Flaxseed is a rich source of alpha-linolenic acid (ALA). Feeding laying hens flax seed can increase n-3 fatty acids content (FA) in eggs, thus increasing human intake of n-3 FA. However, non-starch polysaccharides (NSP), which decreases digestibility of dietary lipids and proteins. Addition of carbohydrase enzymes to flax-based layer diets...
Perinatal growth restriction programs higher risk for chronic disease during adulthood via morphological and physiological changes in organ systems. Perinatal growth restriction is highly correlated with a decreased nephron number, altered renal function and subsequent hypertension. I hypothesize that such renal maladaptations result in altered pharmacologic patterns for life. Pregnant...
Although fish are a nutritious food source, they also are the main source of methylmercury exposure in U.S. populations. This research examined the risks from methylmercury and benefits from omega-3 fatty acids and selenium from fish consumption. The first study provided the first region-specific quantitative risk/benefit analysis for nine commonly...
This dissertation has focused on lignin byproducts that result from the alkali processing of wheat straw. The first study evaluated the application of “standard” lignin quantification methods for summative analyses of alkali-based processes. Mass closure during NaOH processing at 25, 50, and 75°C of wheat straw was evaluated using the...
Two studies were conducted to evaluate the influence of supplement composition on intake and digestibility of a low-quality (< 6% CP), cool-season forage, as well as cow performance. Treatments included a non-supplemented control (CON), corn (approximately 8% CP), corn and urea (LU = corn + 0.09 mg/kg BW urea, approximately...
These studies focused on maximizing the nutrition and safety of raw whole
muscle beef while minimizing quality and shelf-life alteration. The first study used
response surface methodology to predict fluid loss from injected beef strip loins and
steaks as influenced by levels of salt and sodium phosphates (SP) in the...
Vitamin E was first described in 1922 as an unknown factor required for impregnated rats to carry their offspring to term. In fact, when vitamin E was chemically characterized it was given the name "tocopherol" derived from the Greek: tokos = childbirth; phero = to bear; and –ol, indicating an...
Calcium is important to bone health as well as related to a number of health problems such as osteoporosis, hypertension, cardiovascular disease, and some cancers. Calcium intake level was far below the Dietary Reference Intake and dairy product consumption was inconsistent with the Daily Dietary Guidelines among adolescents in Taiwan...
The aim of this study was to investigate the vitamin B-6 status of Saudi adult males and compare the status between rural and urban subjects. Fifty-one adult male subjects were recruited from urban (n=31) and rural (n=20) populations of Riyadh. These subjects were reclassified to cigarette smokers (n=19), water pipe...
Our objective was to determine the influence of CP supplementation frequency
(SF) on cow performance, grazing time, distance traveled, maximum distance from
water, cow distribution, DMI, DM digestibility, harvest efficiency (HE), percentage of
supplementation events frequented, and CV for supplement intake for cows grazing
low-quality forage. One hundred-twenty pregnant (approx....
Two experiments were conducted to evaluate the effects of forage quality and supplemental undegradable intake protein (UIP) level on intake, digestibility, and performance of beef cattle. In Exp. 1, five ruminally cannulated steers (BW = 456 ± 6 kg) were used in a 6 x 5 incomplete Latin square with...
The purpose of this study was to evaluate the effect of parent education on increasing parental awareness of vegetable servings needed by preschool children, lowering barriers to increase vegetable consumption in their children's diets, increasing confidence in their ability to increase vegetables in their children's meals, and promoting parent-child interaction...
Two experiments were conducted to determine the effects of daily (D) vs
alternate day (AD) supplementation of soybean meal (SBM) or wheat
middlings (WM) on forage organic matter intake (OMI), ruminal digesta
kinetics, total tract digestibility, and ADG of steers consuming low quality
forage. In Exp. 1, five Angus x...
We investigated the relationship between calcium and protein intake and bone loss over a one-year period in 99 early postmenopausal women (1-36 months) aged 51.3 ± 0.31 years. Bone mineral density (g/cm²) of the left hip (total hip, femoral neck, greater trochanter) and lumbar spine (L1-L4) as well as body...
Two studies were conducted to determine the effect of varying vitamin B-6 (B- 6) status on lymphocyte mitogenic response and pyridoxal 5'-phosphate (PLP) concentration in peripheral blood mononuclear cells (PBMC) in young women. In the first study, women were fed 1 mg/d for the first week and 1.5, 2.1 and...
The purpose of this study was to examine the effect of extreme exercise on vitamin B-6
metabolism and urea nitrogen. Nine men and five women completed two 5-day trials; Trial 1
(T1) included a 50-km ultramarathon on day 4 and during Trial 2 (T2) subjects were "inactive"
on day 4....
While replacement of dietary saturated fat with unsaturated fat has been advocated
to reduce cardiovascular disease risk, diets high in polyunsaturated fatty acids
(PUFA) could increase low density lipoprotein (LDL) susceptibility to oxidation,
potentially contributing to the pathology of atherosclerosis. To assess in vivo lipid
peroxidation and susceptibility, of LDL...
Current dietary recommendations have placed increasing emphasis on dietary fat
as an important element to decrease risk of cardiovascular disease (CVD). Although total
fat and the fatty acid composition of diets influence the risk of CVD, the optimal amounts
of different fatty acids are not well defined, especially if n-6...
The objective of this study was to compare the effects on eating behavior,
lipids, lipoproteins, lipid peroxidation, and glycemic control in women with type 2
diabetes of a high-monounsaturated fat diet (HM) compared to a high-carbohydrate
diet (HC).
In an outpatient feeding study, ten hypertriglyceridemic postmenopausal type
2 diabetic women...
Twenty-one women, lifelong residents of Xichang County, Sichuan Province, China, an area of very low soil selenium (Se), received tablets containing either 100 μg Se daily as Se-enriched yeast (+Se) or no additional Se (-Se), throughout the last trimester of pregnancy and the first three months of lactation. Diet was...
The effect of vitamin B-6 (B-6) status on plasma fatty acids (FA) levels and lipid
metabolism was investigated in this metabolic study. Eight female subjects were fed for 28
days. For the first 7 days, they were fed a constant diet containing 2.10 mg of B-6. For the
rest of...
Numerous dietary factors have been shown to influence the fatty acid profiles (FAP)
in breast milk from lactating women. However, few studies have evaluated the effect of trace
minerals on milk FAP. Consequently, the purpose of this study was to determine the effect
of selenium status on the FAP in...
This study examined the effect of vitamin B-6 supplementation and exhaustive
submaximal exercise on plasma catecholamine concentrations, and the relationship
between plasma catecholamines and fuel use, heart rate and oxygen consumption. Five
trained men (age= 18-35 years; V0₂max=53 ml 0₂/kg/min.) participated in two controlled
dietary periods that were identical except...
Four studies were conducted to evaluate the effect of varying levels of vitamin
B-6 (B6), protein and pyridoxine glucoside (PNG) on B6 status and requirements of
women. In the first two studies, women were fed a constant protein diet and vitamin
B-6 intakes of 0.84 to 2.39 mg/d during 10-...
Trained male cyclists (6 in study 1, 5 in study 2) cycled to exhaustion (EXH) at
75% of VO₂ max twice; once in the non-supplemented (NS) state and once in the vitamin
B-6 (B-6)(20 mg PN) supplemented (S) state. The diet contained 2.3 mg B-6 in study 1
and 1.9...
Protein microcapsules (PM) were developed as a tool for investigating aspects of dietary protein utilization by mussels, Mytilus edulis trossulus. Digestion of PM in vitro by protease, trypsin, amylase and extracts from the gut of mussels varied significantly depending on the type of protein encapsulated or whether carbohydrates were added...
The purpose of the study was to set up an in vitro model of the colon which
would permit the analysis of cellulose fermentation by human colonic microflora. Studies
of the degradation of polysaccharides by colonic bacteria may help to explain the observed
physiological consequences of consuming dietary fiber common...
Dietary intake data of 15 treated patients with
phenylketonuria (PKU) (7-17 years) and six control siblings
(6-14 years) were used to evaluate the effectiveness of
medical foods to balance energy, protein, vitamin B-6, and
individual amino acids from natural foods and to evaluate
vitamin B-6 status. Four-day diet records were...
This investigation was conducted to determine the
effect of estrogen replacement therapy (ERT) on vitamin B-6
status of postmenopausal women. Nineteen postmenopausal
women served as subjects. Nine (54.7 + 4.7 years) were
taking ERT (experimental group); ten (56.8 + 2.3 years)
were not (control group). For three consecutive days,
subjects...
We investigated the effect of pyridoxine
supplementation on the utilization of protein in a low-protein,
plant-based diet in four subjects (2 men and
2 women), aged 21 to 38 years. Following two days of a
negligible protein diet, this 34 day study was divided into
three dietary periods: the subjects...
The objectives of this study were a) to determine
if pyridoxine (PN) supplementation increases the rate at
which plasma urea and ammonia return to basal levels,
following exercise, b) to determine, by open circuit
calorimetry, the utilization of carbohydrates, and c) to
further understand vitamin B-6 metabolism during and
following...
The study was designed to indirectly understand muscle
glycogen utilization during prolonged exercise when either
glucose, fructose, or water is ingested. Eight trained
adult males exercised on a cycle ergometer at 58±7% of V02
max for 2 h on 2-4 occasions. At 0 minutes of exercise and
at 30-minute intervals...
The effect of pyridoxine (PN) supplementation on lymphocyte
responsiveness was investigated in 15 elderly volunteers (aged
65-81 years) by measuring lymphocyte proliferation to T and B cell
mitogens, lymphocyte subpopulations with monoclonal antibodies
(T3, T4, T8) and plasma pyridoxal 5'-phosphate (PLP) concentration
at pre-supplementation and after 1 and 2 months...
This investigation was designed to add to present understanding
of vitamin B-6 (B6) metabolism during exercise. Ten women, 5
aerobically trained and 5 untrained, were fed 4 controlled diets: a
moderate carbohydrate (49%) (MCHO) for 2 weeks, a high carbohydrate
(63%) (HCHO) for one week, MCH0+B6 for 2 weeks, and...
A study was conducted to determine the effect of dietary protein on
performance of commercial New Zealand White rabbits.
In Part I of this study, three levels of crude protein (17.5, 19
and 20.5%) were fed to lactating does and their offspring during a 12
month period. Total rabbits born...
The bioavailability of vitamin B-6 from four selected
foods was investigated in five men, aged 22 to 25 years,
who were receiving a pyridoxine supplement. The subjects
received a constant diet containing 1.34 mg of vitamin B-6
throughout this five-week study, except on Saturdays and
Sundays when they ate their...
Tests using paired pens of barrows were conducted during a 13 month
period to determine the influence of protein level of the ration on
production traits of market barrows. One member of each pair of six to
ten full- and half-sib pairs was randomly allotted to each pen group;
thus,...
The objectives of this study were to evaluate the feeding value
and potential toxicity of single cell protein (SCP) when fed to
ruminants.
Three in vivo digestibility trials with lambs, one feedlot trial
with steers and one long term feeding trial of high levels of SCP with
sheep were conducted...
The effect of pyridoxine (PN) supplementation on the activities
of erythrocyte alanine aminotransferase (EAlaAT) and aspartate aminotransferase
(EAspAT) was observed in five men, aged 22 to 25 years.
The subjects received a constant diet containing 1.34 mg of vitamin
B-6 Monday through Friday of each week during this five-week study....
The present study was designed to investigate the
effects of exercise on vitamin B-6 metabolism. Four groups
of subjects (intermittent, college, untrained, and high school)
consisting of nineteen male and two female individuals, participated
in the study. The subjects exercised either on a
bicycle ergometer, by running three 1500 meter...
A four months continuous feeding trial was conducted
to evaluate Canola meal as a single protein supplement in
complete dairy rations. Thirty high producing Holstein
cows in the second week of lactation were randomly alloted
in groups of ten to one of three isonitrogenous (15% CP)
and isocaloric (1.6 Mcal/kg...
Eighteen multiparous, high producing (7,836 kg 4%
FCM) Holstein dairy cows were assigned randomly to iso-
.
caloric rations containing either 12 or 23% crude protein
(CP) on day 40 postpartum. After a 10-day adaptation
period, uterine secretion and blood samples were collected
on the day of estrus (day 0-1),...
A series of four trials was conducted to evaluate activated
sewage sludge (AcSS) as a protein source. In the first trial the protein
efficiency ratio (PER) of sun-dried and auto-claved, sun-dried
AcSS was measured with rats. Growth of AcSS-fed rats was extremely
poor as compared to those rats fed casein,...