Metabolic syndrome, characterized by a collection of risk factors including abdominal obesity, insulin insensitivity, elevated plasma triglycerides, hypertension and low plasma high-density lipoproteins (HDLs), is a major contributing factor to the development of cardiovascular diseases and type II diabetes (T2D). Xanthohumol (XN), a flavonoid isolated from Humulus lupulus cones, has...
Understanding poverty as a state of being poor with regard to concretely being unable to meet basic needs such as water, food, clothes, shelter, and essential services (e.g., sanitation, health, and education), its concerted study in the United States not only stands tandem with constitutionally upheld values such as equality...
Weight bias has dramatically increased over the last 20 years
and is as prevalent as bias based on race and gender. The
media is a primary source of weight bias according to a review
of weight-related content within newspapers, popular press
books, television, and movies. Bias is perpetuated through
messages...
Warm, high pressure systems are measured from a weather station on a high elevation peak in the Oregon Coast range during the winter months. Temperature observations on Mary’s Peak show abnormally warm air in relation to the surrounding areas. The goal of this study was to characterize the frequency and...
Winter hardiness in barley (Hordeum vulgare) is a trait targeted by breeding programs in order to expand the potential area of adaptation of fall-sown cereals. Vernalization requirement is an important factor in winter hardiness. A vernalization requirement is an extended period of low temperature required for transition from the vegetative...
The Mount Thompson 7.5 Minute Quadrangle is located in the central Boulder Batholith, between the cities of Butte and Helena. The Boulder batholith is host to numerous metalliferous vein deposits, including those in the Butte District. Recent USGS (EDMAP)-funded geologic mapping at 1:24,000 scale in the Mount Thompson Quadrangle during...
Due to public and regulatory restrictions, genetic containment technology is critical for research and development of transgenic trees in forestry. A multi-year field trial demonstrated that the RNAi-LFY construct successfully induces sexual sterility in 6K10 poplar (Populus alba) without visible effects on vegetative development. However, its limited number of replicates...
Diet History Questionnaires (DHQ) can only provide general information about a person’s nutritional intake. It is unable to cluster groups of people based on diets or lifestyles, nor identify specific foods that may play a role in disease prevention. The aim of this study was to validate the use of...
Microbial electrolysis cells (MECs) for hydrogen production exhibit great advantages over many other biohydrogen production techniques in terms of versatility of substrate and hydrogen yield. However, hydrogen scavenging by methanogens put forward a great challenge to the application of the single chamber MECs when using mixed culture. Various strategies were...
Studies were conducted to assess influencing the effectiveness of sheep for grazing control of false brome (Brachypodium sylvaticum) an invasive grass species. Orphan lambs were exposed to false brome via grass extract in their milk and naturally reared lambs were exposed by grazing dense stands of false brome with their...
Postpartum uterine inflammation (endometritis) is not uncommon in beef cattle, resulting in infertility and culling. Prolonged calving may contribute to the increased incidence of endometritis in heifers. Commonly used methods to treat endometritis in beef cattle include intrauterine antibiotics and/or hormone injections, which can result in meat residues. Platelet rich...
Canine Transitional Cell Carcinoma (TCC) makes up 2% of all canine cancers and is the most common cancer of the bladder for dogs. Usually diagnosed in its later stages, TCC is difficult to treat, with low survival times. Current diagnosis involves invasive techniques, and mainstream screening tests are often ineffective....
Birds of prey and carnivorous mammals feed on small birds, rodents and other small mammals. Humans perceive many of the prey animals of these predators as well as the predators themselves, as “pests”, and seek to control them. Control methods for these unwanted pests, particularly rodents, have the potential to...
We compiled Western Pond Turtle (Actinemys marmorata) and Painted Turtle (Chrysemys picta) locations in northwestern North America, consolidating data from multiple sources including nine U.S. State and Canadian Provincial jurisdictions. We assessed numbers of discrete locations, and analyzed distribution patterns temporally and spatially. Western Pond Turtle observation records ranged from...
The ability to observe species in their natural setting using remote cameras is an increasingly important tool for in-field research. Remote cameras for terrestrial studies are inexpensive and widely available, but there are few comparable options for researching aquatic habitats. For this study, a do it yourself (DIY) underwater camera...
Gene expression in plants (and other eukaryotes) is a very complex process. Gene expression is primarily controlled by proteins and their binding sites near the gene in the chromosomal DNA. More specifically, interactions among transcription factors (DNA-binding proteins) and cis-regulatory modules (CRMs-sets of binding sites) control the expression of nearby...
Native to the Pacific Northwest, Ceratomyxashastais a myxosporeanparasite that infects wild and cultivated salmon and has a significance impact on its population in the Klamath River basin. The study suggests that the transmission of mixed genotypes of the parasite are common in Chinook salmon but the age of the fish...
The rocky shores of the US West Coast are home to diverse ecological communities made up of species that are uniquely adapted for survival at the harsh boundary between land and sea. Even so, physical or environmental stressors regularly kill swaths of animals on the rocks. This is called disturbance....
Stringhalt is a disorder in horses characterized by high stepping with hyperflexion of the hind limbs. This disorder has been reported from the US, Australia, Chile, and Brazil. In most instances, the disease manifests itself in horses exposed to summer dandelion (Hypochaeris radicata). Stringhalt has been induced by feeding summer...
There exists a growing need to establish a better in vitro model for evaluating PAH toxicity and carcinogenicity and to improve assessments of risk to human health, especially to the complex mixtures that exist in the environment. Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants due to the incomplete combustion...
Chitosan based hemorrhage control dressings provide effective external hemorrhage control while having few side effects. Known residual contaminants present in chitosan formulations include endotoxins and proteins known to stimulate gene expression in the liver. This study investigated gene expression in the liver using rat bio-compatibility models to gain knowledge regarding...
The research revolves around membrane-assisted electrolysis to recover caustic soda from alkali processing. This research began with the lab having no experience in electrochemistry and thus conducting initial experiments to begin to understand electrochemical systems. The lab then looked at both gas production and anodic fouling behavior. The lab then...
Vibrio cholerae (Vc), the etiological agent of cholera, has developed many mechanisms to combat the great fluctuations in pH, ion content and osmotic conditions of the vastly differing environments it encounters through its infections of the human host. A series of cation antiporters are found in the Vc membrane to...
Toxicological studies show that oral doses of nickel can cause allergic contact dermatitis, and routes of exposure, such as nickel leached from stainless steel cookware are not well characterized. In this study, four variables: grade of stainless steel, cook time, stainless steel seasoning or cooking cycles, and commercial tomato sauces,...
Yaquina Bay, Oregon, has been introduced to a few The invasive species in Yaquina Bay, Oregon can have a huge effect on the native species and local ecosystem. European green crab (Carcius maenas) is an invasive species. The European green crab can be found on the mudflats competing with the...
Parathyroid hormone (PTH) is directly involved in breast cancer (BC) metastasis, as well as the severity of metastatic tumors. Excess PTH, caused by insufficient dietary calcium, may be an important and preventable factor affecting BC metastasis to bone. PTH levels increase with age in most women, largely due to insufficient...
HafSOx is a dielectric material that also functions as an insulator. It is able to be spin-coated onto a silicon substrate, creating a thin, amorphous film. It is very dense, which helps lead it to a high dielectric constant, making it ideal for electronic devices through the use of lithography....
This gamma irradiation study was conducted to establish an LD50 of the hop (Humulus lupulus L.) cultivar Cascade . The overall, long term goal is to generate replacement hop cultivars by inducing minor but significant DNA alterations with gamma radiation to improve agronomic quality without changing the brewing profile. The...
Respiratory Syncytial Virus (RSV) is a major concern in infants, the elderly, and immunocompromised individuals. There is currently no vaccine available. While it causes a significant number of hospitalizations and complications, RSV is a thermo labile virus and not very stable in the environment. This matter complicates the creation of...
Zinc is an essential micronutrient required for many cellular functions, including
the development and function of the immune system. The biological consequences of
zinc deficiency illustrate the significance of zinc in human health. Zinc deficiency has
been shown to cause the dysregulation of both innate and adaptive immune responses, as...
Understanding the mechanisms through which stem cells self-renew is important for the development of new therapies to repair and restore damaged tissue. This research explores possible mechanisms of telomere mimic action and seeks to validate a new assay for evaluating telomere binding antagonists and studying the effects of telomere binding...
As engineered nanomaterials (ENM) become more common in consumer products, it is crucial to rapidly assess toxicity of unique ENM. Previous studies rarely focused on standardizing nanotoxicity testing, leading to inconsistent and irreproducible results. This study characterized a variety of ENM of different size, shape, core type, and surface charge...
In this study, the effects of A and B site cation substitution of the distorted perovskite YCoO₃ on crystal structure and thermoelectric properties were investigated. Polycrystalline samples of Y₁₋ₓCaₓCoO₃ (0 ≤ x ≤ 0.20) and YCo₁₋ₓRhₓO₃ (0 ≤ x ≤ 1.0) were prepared by solid state reaction. Optimal synthesis conditions...
Cancer is class of diseases that has great diversity and unfortunately is one of the leading causes of death in the United States. Chemotherapy remains an important cancer treatment modality, and superior chemotherapeutic drugs are constantly being sought. Gemcitabine is an anti-tumor chemotherapeutic drug and it is the first line...
In this study, we compared the behavior of bottle fed lambs verses mother fed lambs in three testing scenarios; the sociability test, the paired attachment test, and the mismatch attachment test. The tests sought to determine if a familiar human can serve as a secure base for lambs in a...
As global temperatures continue to rise there has been an increase in forest fire frequency and severity. With larger areas of forest being burned it is increasingly important to understand how forest fires effect snow processes. Previous research shows that burned areas will accumulate more snow and that this snow...
Saccharina sessilis, lettuce kelp, is a macroalgae that contributes to the primary production in the intertidal zone off of the Oregon Coast. Macroalgae can serve as a bioindicator of increased nutrient input, water quality, and shifts in the intertidal environment. Competition for light exists between macroalgae species and with other...
Abstract
Haptoglobin is an acute phase protein that is elevated in response to tissue damage and infections in dairy cows. The objectives of this study were to evaluate whether serum haptoglobin concentrations in the first week after calving can indicate disease status and severity, and whether haptoglobin concentrations are elevated...
Throughout Cane, a colon is representative of both characters’ thought and dialogue in a quasidrama technique that perpetuates the idea of a staged performance (Foley 233). Through this form, Cane encapsulates the non-authentic expression that African Americans were subjected to by a means of acting and/or pretending in fear of...
The idea for this research projected stemmed from research I conducted during my Economics 439: Public Policy course. This research project examines the idea of Voter Identification Laws and Regulations and their effect on voter turnout and participation in the United States of America. I previously found that economists studied...
A research project looking into the feasibility and impact of adding a mentorship program to improve the academic and professional success of international students in the Oregon State University College of Business.
Hypoxia is a naturally occurring phenomenon that happens seasonally off the Oregon coast. As a consequence, there has been a great deal of research focused around analyzing the strength and duration of the hypoxic event during the summer season. This paper, however, takes an innovative approach by looking at the...
Bio-oil from hydrothermal liquefaction (HTL) represents a promising fuel source due to large higher heating values and compatibility with current energy infrastructure. For bio-oil production to become commercially viable, novel methods of co-product recycling must be explored.
The aims of this study were to culture Chlorella vulgaris, a common HTL...
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a potent immunosuppressant and a prototypic ligand for the aryl hydrocarbon receptor (AhR). In a graft versus host (GvH) response, treatment with TCDD suppresses a cytotoxic T-lymphocyte (CTL) response by Day 10, concurrent with a T-regulatory (Treg) like phenotype in the donor CD4+ T-cells, observed on Day...
Abstract
Flame-retardants (FRs) generally enter the environment by dissociating from the products they were originally manufactured into. The predominant class of FR used in consumer products before their eventual phase-out in the mid-2000s were the polybrominated diphenyl ethers (PBDEs). Previous studies have illustrated a correlation between socio-economic status, notably gross...
Vibrio cholerae a major health problem in developing countries because of its ability to cause the disease cholera in humans. As V. cholerae bacteria are common inhabitants of temperate marine and brackish water environments worldwide, they are subject to intense top-down pressure by amoebae, which can affect their survival and...
The goal of this project was to develop and test non-herbicidal techniques that remove nonnative plant or weed seeds from the top 2 inches of the soil. The Nature Conservancy (TNC) is currently restoring prairies in the Willamette Valley. Some areas of the Pacific Northwest have already seen loss of...
Polycystic Ovary Syndrome (PCOS) is an idiopathic disorder prevalent in ~5-10% of reproductive-aged women. In PCOS women who become pregnant, many experience complications including increased susceptibility of having a miscarriage, high blood pressure, and gestational diabetes, among others. Additionally, exposure to PCOS may have transgenerational impacts: offspring may be at...
Spiropyrans are a family of photosensitive molecules their hydrophobic properties when irradiated with light. Since spiropyran can be controlled by light, it can be used to regulate transport across membrane media. The ability to control the release of a substance onto a platform is promising in reducing the cost of...
BACKGROUND: Oregon produces 99% of the hazelnuts grown in the United States. The key pest threatening commercial hazelnut orchards in Oregon is Cydia latiferreana (Tortricidae), filbertworm moth (FBW). Currently, most growers control FBW with one to two applications of esfenvalerate (Asana XL), a broad spectrum pyrethroid. Pheromone mating disruption is...
A literature review for a research project being done in partnership with the Oregon Department of Fish and Wildlife and the Columbia River Intertribal Fish Commission, which will look at non-breeding California sea lion males in the Columbia River Basin that have been preying on migratory salmon. This will be...
In order to reduce the cost of lignocellulosic ethanol production, a model was previously developed to predict the amount of fermentable sugar released based on parameters concerning both biomass and enzymes. To validate this model, enzymatic hydrolysis must be carried out using individual purified cellulase enzymes. Endo-1,4-β-D-glucanase I (EG I),...
This thesis looks at the third harmonic voltage response of a small incandescent lightbulb. The third harmonic voltage, or 3 omega (3ω) voltage, arises from applying a 1ω sinusoidal voltage across a resistive material. The 3ω voltage carries with it information about the thermal conductivity of the material. A lock-in-amplifier...
BCL11B, a gene regulatory transcription factor, is known to be a regulator of T cell development in the immune system, and has been identified as a tumor suppressor for T cell leukemias. Defining the molecular events that regulate the activity of BCL11B will provide potential drug targets that will aid...
Anaerobic digestion of lipid-extracted algal biomass has been proposed as a method to
recover and recycle nutrients in algal biofuel systems. The purpose of this study was to quantify recoverable nitrogen and phosphorus from anaerobically digested C. vulgaris. Recoverable nutrients were deemed those present in the liquid phase of anaerobic...
Oregon White Oaks (Quercus garryana) were once abundant in the Willamette Valley, Oregon, but have drastically decreased because of fire suppression, agriculture, and human development. Oaks are an important ecosystem because they provide a diverse habitat for bird species that are insectivorous and/or cavity nesters. We studied the oak restoration...
To model the behavior of the spring run Chinook salmon, we expanded the current dataset of salmon counts at the Winchester Dam, Winchester, Oregon, to include historical data from 1991 through 1997. These counts were used to identify Chinook salmon behavior that resulted in defining preferable water conditions during their...
Research in Dolan lab focuses primarily on the adaptive immune system and ways it can be better utilized to eliminate infected or transformed cells. Research focuses on the major histocompatibility (MHC) class I antigen presentation pathway, which plays an important role in alerting the immune system of the presence of...
Widespread mercury contamination threatens aquatic ecosystem functions due to its capacity to bioaccumulate, biomagnify, and induce toxicity in food webs. Methylmercury (MeHg), the organic form of mercury, bioaccumulates more efficiently and tends to be more toxic than the inorganic form. One important impact of mercury exposure is inhibition of effective...
The AhR is a ligand-activated transcription factor that mediates the potent immunosuppressive effects of TCDD. AhR-dependent changes in gene expression appear to alter the differentiation of CD4+ T cells to induce a population of CD4+CD25+ T regulatory cells (Tregs) that suppress the immune response. Ascertaining if and how the AhR...
Epidermal RNA samples from FVB/N mice treated with PAH standards and
environmental PAH mixtures were harvested and purified using Trizol extraction and
RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5),
indicating significant RNA degradation and the need to modify handling, collection, and
processing of skin...
Cystic ovarian disease (COD) is one of the main causes of infertility in dairy cattle and has a high economic impact on farmers. COD is caused by an endocrine imbalance within the hypothalamic-pituitary-ovarian axis which prevents the mature Graafian follicle from ovulating. The cause at the molecular level is not...
IODP 351 cruise to the back arc region of the Izu Bonin contained cores with tephra layers intermitted within. These tephra layers under went electron micro probe analysis to gather major earth element abundances in order to put a parameter of a. where did the tephra originate from (volcanic source)...
To initiate the defense response to an invading pathogen, plants utilize an array of immune receptors to recognize virulence effectors. Virulence effectors are released by pathogens to suppress immune responses in target hosts. These effectors are recognized by a family of resistance proteins known as nucleotide-binding leucine-rich repeat (NB-LRR) proteins....
The Boulder Batholith and Elkhorn Mountains Volcanics (EMV) formed concurrently during shortening between ~85-76 Ma near the end of Mesozoic Cordillera arc magmatism. Together they represent an exceptionally well-preserved and voluminous record of continental arc magmatism. We report field and analytical results from studies of the EMV and Boulder Batholith...
Pactamycin, a complex aminocyclitol compound produced by Streptomyces pactum, has significant antibiotic, antitumor, and antiplasmodial activity. However, this natural product has not been developed for clinical use due to its high cytotoxicity. Modulation of its biological properties by chemical synthesis has been difficult due to its complex chemical structure. Therefore,...
A better understanding of the distribution and spawning behavior of Crangon shrimp and their relationship with their predators, including the green sturgeon, Acipenser medirostris, is an important step toward the larger goal to understand food webs of the nearshore continental shelf of the northeastern Pacific. Here, I examined how the...
Genetic and environmental factors influence individual cancer risk. Mutations in DNA mismatch repair (MMR) genes increase colon cancer risk. Exposure to carcinogens, including the ubiquitous polycyclic aromatic hydrocarbons (PAHs), also is associated with increased cancer risk. To understand the combined effects of both environmental exposures and genetic risk, we investigated...
BioResource Research thesis defense presentation for Tyler Sato Spofford.
The COVID-19 pandemic highlighted disparities in land access and food security. Research has shown that access to gardening reduces financial burdens on low-income households. Compared to homeowners, individuals and families in rental housing face added barriers to vegetable gardening and growing...
Pesticides are ubiquitous, with more than one billion tons of pesticide products used in the United States annually. These compounds are characterized by their toxic effects to the target organism; however, pesticides are also well known for their deleterious effects to non-target species. Because pesticides have ecological and human health...
Microplastics (<5mm) are a ubiquitous environmental pollutant that have the potential to cause significant harm in many fish species. Therefore, understanding the quantities at which they are being consumed by living organisms is crucial for characterizing risk. There are currently gaps in the microplastic literature regarding microplastic data from finfish...
4-H, an agriculture based youth program, promotes a variety of curricula in both agriculture and Science, Technology, Engineering and Math (S.T.E.M.) topics. However, science based curricula provided by this organization gravitates away from the programs agricultural roots. An effort was made to pilot a test on lesson plans that would...
Energy production and consumption is a major environmental and economic concern in the world today. Biofuel produced from lignocellulosic biomass is being explored as an alternative energy source to petroleum. Lignocellulosic biomass is comprised of cellulose, hemicellulose, and lignin. To produce biofuels, lignocellulosic biomass must be broken down to expose...
Lignocellulosic biomass has potential as a renewable resource for global energy and bioproducts production. Biomass consists of three main polymers, cellulose, hemicellulose, and lignin. Cellulose is a homopolymer of glucose, while hemicellulose and lignin are heteropolymers. Lignin is primarily made of syringyl (S), guiacayl (G) and p-hydroxyphenyl (H) units. Pretreatment...
Tumor necrosis factor alpha (TNF α) and interferon gamma (IFN γ) were initially recognized as inflammatory mediators produced by immune cells in response to infectious agents or foreign material. (1,5) However, over the past decade much research in mammalian species has indicated that these proteins also play key roles in...
OVERVIEW OF INTRODUCTION - This is a combined thesis for both disciplines of BioResource Research (BRR)Interdisciplinary and International Degree (ID) Programs OSU. The first chapter encompasses the BRR research, which is a preliminary study utilizing immunohistochemical method to characterize melanomas in bigenic mice with activated Cdk4 protein and Retinoid-X-Receptor α...
Fusarium solani is a chronic disease that causes root rot which affects bean production worldwide. Recently, a QTL map was generated which identified several SNPs which were found to be associated with resistance to the pathogen. To be useful in a breeding program, INDELs must be identified which co-seggregate with...
Symbiotic relationships between cnidarians and dinoflagellates within the genus
Symbiodinium are critically important for the success of coral reefs. These symbionts
provide their hosts with photosynthetically-fixed carbon in exchange for nutrients
and shelter. Symbiodinium is a phylogenetically diverse genus that is often typed by
sequencing the ribosomal internal transcribed spacer...
Renibacterium salmoninarum is the causative agent of bacterial kidney disease in both
wild and farmed salmonid species worldwide. The genome of this pathogen has
significant synteny to the ubiquitous, soil-dwelling Arthrobacter spp. though it is 1.9 Mb
smaller, suggesting that reductive evolution has occurred. Recently, our group finished
sequencing and...
Four root mutants, diageotropica (dgt), and altered root architecture (ara) 1, 2, and 3, were used to study root architecture and better understand the process by which root architecture is determined. Adventitious root formation, tissue response to auxin, nutritional response, and starch accumulation in the leaves were tested in ara...
Mammalian female reproduction requires preovulatory surges of gonadotropin-releasing hormone (GnRH) from neurons in the preoptic area (POA) of the hypothalamus, initiated by elevated estradiol (E₂). Rising E₂ activates a subset ofsexually dimorphic Kisspeptin neurons in the female, located in the anteroventralperiventricular nuclei (AVPV). Conversely, E₂ negative feedback is mediated by...
Cancer economic impact is enormous and depending on the stage in which a colon cancer
is detected, five-year survival rates for colon cancer may be as high as 74%, or as low as
6% (American Cancer Society, 2012). Thus early prevention of colon cancer is crucial to
maximize the chances...
Embryonic development, where organs and major structures begin to develop, requires precise coordination of numerous gene regulatory pathways. Fendrr is an long non-coding RNA (lncRNA) that plays a role in regulating gene expression in mesodermal tissues during early embryonic development in mouse. The lateral plate mesoderm (LPM) is an earlier...
Endophytes, foliage inhabiting fungi, are an unexplored source of fungal biodiversity and a potential resource for the production of bioactive natural products. In this research, leaf tissues from the species Arbutus menziesii, Rhododendron macrophyllum and the genus, Ilex and Salix were collected for the isolation of endophytic fungi. Fungal extracts...
Mammary tumors are the third most common tumor in cats after cutaneous neoplasia and lymphosarcoma. More than 80% of feline mammary tumors are adenocarcinomas, which are similar to human mammary adenocarcinomas in their biologic behavior and histologic characteristics. Despite treatment with radical mastectomy, feline mammary adenocarcinoma is associated with a...
Research Outline
•Amplify the coding region of the spike protein by PCR using primer flanking the S protein coding region
•Clone the coding region into PCR-TOPO 2.1 cloning vector
•Re-clone the coding region into PET6XHN-N vector
•Induce the spike protein expression in BL-21 cells
Giardia and Cryptosporidium protozoans are resilient, zoonotic waterborne pathogens that can bypass water treatment and kill millions of people and animals annually. Giardia and Cryptosporidium cysts and oocysts, respectively, can spread through feces; poor sanitation and poverty can increase the risk for giardiasis and cryptosporidiosis diarrheal diseases. We are currently...