Pasteurization radiation offers a new means of extending the
refrigerated storage life of foods. This procedure avoids many of
the undesirable changes which result when sterilization radiation is
used and still eliminates most of the spoilage organisms.
Shrimp was treated with 5 ppm chlortetracycline and subjected
to pasteurization levels of...
The over-tenderization of irradiated-sterilized meat is one
of the more important adverse effects resulting from the use of
irradiation for the preservation of meat. Although research has
been reported on the activity and effect of the inherent proteolytic
enzymes of irradiated meat, very little work has been directed toward
the...
Compounds belonging to the vitamin K family possess anti-hemorrhagic
property, and are used in treating patients suffering
from hypoprothrombinemia and obstructive jaundice. Some of
these compounds also exhibit marked antimicrobial activity toward
various microorganisms. Vitamin K₅, 4-amino-2-methyl-
1-naphthol hydrochloride, a water-soluble analog of vitamin K
has been shown to possess...
The storage life at 43°F of ground dover sole fillets (Microstomas pacificus) was determined microbiologically after treatment with
ionizing radiation and antimicrobial agents. Sodium benzoate, potassium
sorbate and the sodium salts of the methyl and propyl esters
of parahydroxybenzoic acid (MPB and PPB) all approximately doubled
the refrigerated storage life...
The two naturally occurring cyclopropene fatty acids, sterculic
and malvalic, have been synthesized. The cyclopropene ring was
made by inserting a methylene unit into an acetylenic linkage.
The synthesis of methyl sterculate was repeated using diethyl
aluminum chloride, diazomethane, and methyl stearolate.
Methyl malvalate was totally synthesized. Diethyl malonate
was...
Certain chlorinated insecticides can be identified by electron capture gas-liquid chromatographic (EC-GLC) analysis of the prepared sample before and after treatment with ultraviolet irradiation, Upon U.V. treatment, characteristic degradation products arise from the insecticides investigated. Comparison of the degradation pattern obtained by EC-GLC of unknown and authentic insecticides provides an...
Effects of high temperature aging upon certain characteristics
of bovine l. dorsi muscle were studied. Paired wholesale ribs of
carcasses were obtained subsequent to slaughter. The left rib of
each pair was held at 30°C for 24 hours, then stored at 3°C.
Analogous right ribs were immediately stored at 3°C....
Diets containing cyclopropenoid fatty acids (CPFA) were fed to
rainbow trout. At a level of 223 ppm (from Sterculia foetida oil),
these CPFA in six weeks reduced weight gain by as much as 50 percent
over the control fish on the same diet without CPFA. Compounds
containing the intact cyclopropene...
The composition and some qualitative aspects of ground beef
offered for sale in Corvallis, Oregon were studied. Samples were
purchased from six retail markets twice weekly throughout a six
week test period. Three stores were independently owned and operated
and three were chain stores.
Differences in moisture, fat and protein...
The electrophoretic behavior of sarcoplasmic proteins was
investigated by the use of vertical acrylamide gel electrophoresis.
The first part of the study was concerned with the separation of
sarcoplasmic proteins extracted at different times of post-mortem
aging. Electrophoresis of the sarcoplasmic extracts by the discontinuous
technique, using ten percent acrylamide...
Studies involving freeze-dried methyl linolenate-gelatin foams indicate that the extent of lipid-protein interaction corresponds to the degree of autoxidation of the lipid fraction. It appears that approximately 11% of the malonaldehyde as defined by the 2-thiobarbituric acid test is firmly bound to the protein. The induction period of autoxidation is...
Seven species of microorganisms indigenous to fish were inoculated into sterile fish muscle, obtained from Sebastodes alutus and Sebastalobus alascanus, and the production of total volatile acids (TVA) and total volatile bases (TVB) was determined during storage at 1 C. No production of TVA and TVB was noted in sterile...
Pesticide chemists have recognized since the 1940's that ultraviolet light was deleterious to the insecticidal properties of certain compounds. However, it has been only within the last 15 years that a number of photodecomposition products of the chlorinated hydrocarbon
insecticides have been isolated and identified. The degradation of insecticides by...
A threshold pattern for the even-numbered free fatty acids (FFA) in butter depended on chain-length. Butyric acid had the lowest total average flavor threshold (AFT) of the more volatile FFA, and the total AFT values increased as chain-length increased through hexanoic and octanoic acids. A soapy after-taste predominated at and...
Thermal oxidation of methyl oleate was studied over a range of temperatures from 50 C to 150 C for periods of time up to 30 min. Degradation was quantitatively followed by gas liquid chromatography (GLC) and liquid scintillation counting of the products of methyl oleate-U-14C heated under a stream of...
Dimethyl sulfide, acetaldehyde, isobutyraldehyde, furan, 2-furaldehyde, 2-acetyl furan, and ethyl furoate were produced when pureed strawberries were heated to 120° C. for 30 minutes. Shorter heating periods were sufficient to produce the first three compounds. With the exception of acetaldehyde, none of the heat-induced compounds were detected in unheated, pureed...
Cranberries are processed mainly for the production of jelly,
sauce and juice. The content of pectic substances in the cranberry
gives the fruit a desirable property for processing such products.
Pectic enzymes which catalyze the hydrolysis of pectic substances
may affect the consistency of these products.
The concern of this...
Eighty-one microbial species isolated from seafoods and other marine sources were examined to determine the extent of interactions among these species. Spot-plates, cross-plates, and concurrent growth experiments at 7, 15, 20, and 30 C indicated that Lactobacillus species were capable of inhibiting other microorganisms. Lactobacillus species isolated from oysters and...
Due to the scarcity of information concerning the oxidase systems
in cranberries the peroxidase, catalase, and polyphenolase systems,
which are used as biochemical indexes of adequacy for enzyme inactivation
in other fruits and vegetables, were investigated.
Enzyme extracts were prepared from acetone powders with and
without phenol-binding agents such as...
Selected parameters that affect crude toxin production in both
primary culture (growing cell culture) and resting culture were
examined during efforts to produce sufficient crude toxin for purification.
The parameters included carbohydrate and label source and
concentration, cell concentration, incubation time and temperature
and shaker speed. A higher yield of...
Studies were conducted to determine the post-mortem stability
of lysosomes in the white muscle of rainbow trout (Salmo gairdneri)
and to determine whether lysosomal stability was related to rigor
mortis and its subsequent dissolution. In addition, isolated lysosomes
were subjected to different environments in an attempt to detect some
of...
The reaction of several primary amines with nitrite was investigated
with regard to the formation of N-nitrosamines. When reacted
in a low moisture system at 160°C with a 1:2 molar ratio of amine to
sodium nitrite, various primary amine hydrochloride salts were found
to give rise to heterocyclic nitrosamines. The...
Proteases present in shrimp processing waste are factors in the
technology of shrimp. The distribution of proteolytic activity in
shrimp parts was determined using hemoglobin and casein as substrates. The effects of various parameters upon activity of proteases
in the inedible portion were determined using muscle protein as a
substrate....
Cyclopropenoid fatty acids (CPFA) are natural components of
cottonseed oil, a major food oil in the United States. The ability of
CPFA to cause abnormal biochemical and physiological effects when
fed to laboratory and farm animals has prompted an investigation of
their effects on mice.
Between 0.05 and 0.55% CPFA...
The influence of polyphosphates upon the emulsification of
enzymatic hydrolyzates of casein and lactalbumin and upon salt-soluble
meat proteins was determined by a model system in which
oil-in-water emulsions were formed.
Sodium acid pyrophosphate, sodium tripolyphosphate and
sodium hexametaphosphate were mixed together in a weight ratio
of 4:2:4, respectively, to...
The reactions between sodium nitrite and the polyamines
spermidine and spermine were investigated. When reacted at 80°C
for 1 hr at pH 3.5 with a 1:3 molar ratio of amine groups to sodium
nitrite, five volatile nitrosamines were identified as products in the
nitrosation of spermidine. 3HC1, and two volatile...
Triplicate groups of rainbow trout (Salmo gairdneri) fed six
diets; five based on fish protein concentrate (FPC) ranging in caloric
density from 4.36 to 6.19 kcal/g, and one based on vitamin free
casein with a caloric density of 5.26 kcal/g were evaluated with
respect to diet ingredient utilization. The parameters...
Porapak Q, Chromosorb 102, and Tenax GC, three porous
polymers commonly used as adsorbents in headspace analyses were
investigated. The retention times of various low-boiling compounds
relative to water were measured on the collection columns to determine
which compounds would be lost during the water removal step
employed after sampling...
The development of whey beverages including whey wine has been
an area of whey utilization research. One study to produce a commercially
salable wine from sweet whey, particularly cheddar whey, was
undertaken. It included developing a process to clarify the naturally
cloudy wine, monitoring the physical and chemical changes which...
The effect of washing machine separated rockfish flesh on
composition, quality and desirability was investigated.
Washing machine separated rockfish flesh resulted in a
36.88% loss of solids. The greatest reductions were found in the ash
(80.32%) and lipid (65.13%) levels in the washed flesh. Most of the
sarcoplasmic proteins were...
The objective of this investigation was to develop an acceptable
aspartame sweetened orange sherbet which might offer significant
caloric reduction and cost advantages, and hence be applicable to
commercial production.
A control sherbet, with a preference level equal to a
commercially available orange sherbet was used as the reference.
An...
The proteolytic enzymes in the visceral organs of albacore tuna
fish (Thunnus alalunga) were studied, initially as an extract of the
whole viscera, then as extracts of the individual organs. Preliminary
studies indicated three pH optima of activity in the whole viscera
extracts pH 1.7, 3.1-3.5 and 9.5. Analysis of...
Cyclopropenoid fatty acids cause several physiological disorders
in rainbow trout and rats. Rainbow trout appear to be more sensitive
than rats to the effects of cyclopropenoid fatty acids. Carbon-14
labeled sterculic acid with label in the methylene position of the
cyclopropene ring was injected into the stomach of rats and...
Corn oil (CO) and Sterculic foetida oil (SFO) fed rats were
injected with [9, 10-methylene-¹⁴C]sterculic acid. Less than 1%
of the label was expired as carbon dioxide. The majority of the
label was excreted in the urine as short-chain dicarboxylic acids with
an intact cyclopropane ring. The major metabolites for...
The purpose of this study was to characterize the nonvolatile,
water soluble, bis(hydroxyalkyl)N-nitrosamines produced from the
reaction of spermidine and nitrite.
The biologically occurring polyamine spermidine was reacted
with nitrite in the presence of acid. The reaction products were
isolated and converted to trimethylsilyl (TMS) derivatives. The
derivatized products were...
A dose related carcinogenic response was established for dimethylnitrosamine
administered in the diet of rainbow trout (Salmo gairdneri).
An equation was derived for the relationship between dose and hepatocellular
carcinoma incidence. From a published dose response study
using Porton rats as a test animal, a second equation was derived
for...
Stability, substrate and inhibitor specificity and electrophoretic
properties of a crude polyphenol oxidase (PPO) preparation extracted
from d'Anjou pears (Pyrus communis L.) were investigated. Levels
of polyvinylpyrrolidone and pH of buffer for extraction were found
to affect the specific activity of the extracted enzyme. An extract
prepared with 1.5 g...
Investigations were conducted to determine the nature, type,
functional properties and the amounts of different proteins found in
bovine rumen tissue.
Data concerning the effect of pH and salt concentration on
the extractability of proteins from rumen tissue indicated that 92%
of the proteins could be extracted in aqueous solutions...
The optimum conditions for the proteolytic solubilization of
trash fish and processing carcass waste in a simple shell-in-tube
heat exchange system was investigated. The nutritional and chemical
effects of processing variables were evaluated.
An exogenous source of proteolytic enzymes (albacore tuna
viscera, Thunnus alalunga) yielded a linear increase in the...
In order to determine the source of bacteria in Tillamook Bay,
Oregon, water samples were collected monthly for six months during
the rainy season from October 1975 through March 1976 from the bay
and its tributaries, the Kilchis, Trask, Tillamook, and Wilson
Rivers.
Fecal coliform levels of these samples were...
The formation of 3-hydroxy-N-nitrosopyrrolidine from decarboxylation
of 4-hydroxy-N-nitrosoproline was demonstrated in model
heating system designed to simulate the conditions of frying bacon.
The identification of the nitrosamine was achieved by comparison of
its TLC R [subscript f] value, gas chromatographic retention time, and mass
spectral fragmentation pattern with those of...
Flavor problems associated with the use of cheddar cheese
whey in the formulation of ice cream and other food products were
investigated. During spray drying of whey, which transforms bulky
liquid whey into an easily transportable and storable powder,
various degrees of off-flavor development may occur depending on
the care...
Bacillus subtilis α-amylase was used to hydrolyze
starch from peeled and whole potatoes (Solanum tuberosum).
Effect of gelatinization temperature, pH, enzyme concentration,
temperature of hydrolysis and presence of calcium
ions were investigated. Optimum conditions for hydrolysis
were when α-amylase levels were 0.3 percent of the starch
concentration in the presence...
Micro-organisms associated with gari, produced in Cameroun,
Africa, by the traditional method and that produced in the laboratory,
were isolated, identified, and screened for their abilities to produce
gari flavor. Gari production, under the controlled conditions was
attempted utilizing these isolates and the dairy starter cultures.
Some of the volatiles...
Two methods of separating sugars and acids of Bartlett pears
were compared to decide upon a working method for later studies
involving identification and quantitation of sugars and acids during
ripening of pears and in the production and storage of pear concentrate.
The first method involved precipitation of acids with...
Flavor profiles of canned and frozen whole kernel sweet corn were
obtained by entraining the volatile compounds on Porapak 0 traps and
by subsequent analysis on temperature-programed, 500' by 0.03" ID
capillary columns (SF-96 or Carbowax 20M). Identification was based
on GC/MS data, retention indices (I[subscript E]'s) and retention times....
The nature of a natural antioxidant present in shrimp was
investigated.
Shrimp extracts were prepared using different solvents to
ascertain the best extraction method. The antioxidant activity was
determined using the (β-carotene-linoleate model system and the
oxygen weighing method. Ethanol proved to be the best solvent for
extracting the natural...
The effects of ambient temperature storage on the chemical
and nutritional characteristics of phosphoric and sorbic acid-stabilized
fish autolysates were determined. Variations in sample
composition due to autolysis, deboning, and acidification were also
evaluated.
Autolysates of English sole (Parophrys vetulus), true cod
(Gadus macrocephalus), Dover sole (Microstomus pacificus), and
orange...
Dietary protein intake has been observed to influence the induction
of cancer in animals exposed to carcinogens, presumably by affecting the
activities of enzyme systems which metabolize them. One such carcinogen
is aflatoxin B₁ (AFB₁) a mold metabolite and frequent contaminant of
foodstuffs. Variations in the levels of dietary proteins...
One hundred and fourteen strains of Moraxella species isolated
from marine fishery sources were investigated to determine the cause
of this organism's predominance in seafood. The nutritional requirements,
effect of pH, NaCl concentration, and temperature on growth,
utilization of carbohydrates, protein, and lipid, resistance to 12
antibiotics, sensitivity to phenethyl...
Forty Arthrobacter species isolated from Pacific and Atlantic fishery sources were examined to determine their significance in seafood. All strains were able to utilize inorganic nitrogen as the sole nitrogen source and grew in the absence of vitamins or growth factors. In addition, 78% grew on a medium containing only...
Using phenolic scavengers different methods were examined to determine the optimum conditions for extracting polyphenol oxidase (PPO) from d'Anjou pears. Efficiency of the extraction was evaluated based on browning of the extract, PPO activity per g of pear tissue, absorption scan of 410 nm to -240 nm, and the isozyme...
Dietary fiber has been suggested as interfering with
mineral utilization. The influence of broccoli florets and
wastes, cauliflower heads and wastes, wheat bran and Metamucil
on mineral uptake by the rat was determined using
balance studies. All results were compared to control
animals fed diets containing 10% hydrolyzed cellulose.
Utilization...
This study was designed to investigate the rate of pH decline, changes in protein solubility and in the water-holding capacity (WHC) of beef muscles subjected to pre-rigor electrical stimulation. Samples for the experiment were obtained from animals slaughtered at the Meat Science Laboratory, Oregon State University. Nine good quality steers...
A dietary level of Aroclor 1254 (100 ppm) was fed to rainbow trout (Salmo gairdneri) for 15 weeks to determine the effects on hepatic microsomal enzyme induction. Fish were also fed combined polychlorinated biphenyl (PCB) (100 ppm) and cyclopropene fatty acids (CPFA) (50 ppm) to determine the effects on mixed...
Wines were made from Pinot Noir and White Riesling grapes
which had received field application rates of 2 and 4 lb./acre of the
experimental bird repellent Mesurol (methiocarb or 3, 5 dimethyl-
4(methylthio)phenylmethylcarbamate). In addition, Pinot Noir must
and press juice and White Riesling press juice were fortified with
15...
Several compounds have been shown to induce the mixed function
oxidase enzyme system of animals, and also to inhibit tumor formation.
Included in this category are several vegetables and isolated
vegetable components from the Cruciferae family. In this
study rainbow trout were fed blanched freeze-dried cauliflower,
broccoli, or Brussel sprouts...
Aflatoxin B₁ (AFB₁,) is a potent liver carcinogen to a number
of animal species including rainbow trout (Salmo gairdneri).
Microsomal activation is required for the in vitro conversion of
AFB, to a reactive metabolite, thought to be the AFB₁,-2,3-oxide,
which will bind nucleic acids, produce toxicity, and cause mutagenesis.
Nucleic...
The Enterobacter are often found in the feces of man and
other warm blooded animals but have limited use as indicators
of fecal contamination of foods and water since they are also
often found as natural flora of non-fecal environments such
as water and plants. This study proposes a method...
Peas (Pisum sativum) are an important constituent in the diet
of many people. Peas may be cooked and eaten as green peas shortly
after harvest, stored for short periods in the pods, or, after suitable
treatment, stored for long periods before consumption. Many factors
influence the yield and quality of...
When sulfur dioxide is used in commercial drying of fruits in
combination with low storage temperatures, browning and microbial spoilage
can be inhibited. However, the relationship between moisture level,
storage temperature, and sulfur dioxide content on the color of dried
fruit is not known; therefore the purpose of this research...
This investigation was designed to study the possible effects of
the electrical stimulation of beef muscle on microorganisms present on
the meat surface during and after treatment. The effect of electric
current on microorganisms in minimal media suspension was also studied.
Cell viability before and after electrical treatment or exposure...
The ingestion, uptake, and metabolism of liposomes by juvenile
Pacific oysters (Crassostrea gigas) were studied by several methods in
an effort to assess their potential as encapsulating agents. Liposomes
composed of egg phosphatidylcholine-cholesterol-stearylamine (7:1:2)
formed readily and appeared stable in 20°/oo seawater. Radiotracer
studies with liposomes made with ¹⁴C-labeled cholesterol...
The proteolytic enzymes in the muscle fluid of Pacific whiting
(Merluccius productus) were studied and compared to those found in muscle
fluid of true cod (Gadus macrocephalus). Preliminary studies indicated
three pH optima of activity for whiting, pH 3.5-3.9, 4.3-4.6 and 7.1-7.2.
Only two pH optima were found for the...
Previously, it has not been possible to obtain a concentrated ripe Bartlett pear essence. From studies of Bartlett pear aroma characteristics, it was found that methyl and ethyl decadienoates are the components contributing to the characteristic ripe Bartlett pear aroma. It was also found that only perfume-like aroma products, lacking...
Trimethylamine-N-oxide (TMA-0) reductase activity of resting cells
of Escherichia coli was inhibited by tetrasodium ethylenediaminetetraacetate
(Na₄EDTA), benzoic acid (BA), and methylparaben (MP). The 50% inhibitory
concentrations of Na₄EDTA, BA, and MP were 20.2, 1.2, and 32.4 mM,
respectively. BA at pH 6.5 or below most effectively inhibited the TMA-0
reductase....
Current retail food distribution practices, microbial quality of
bakery products, and the potential for growth of food borne pathogens
(Staphylococcus aureus, Salmonella typhimurium, and Bacillus cereus)
were examined using pumpkin pie as a model.
The pumpkin pies, as obtained from the retail outlets, showed contamination
with food pathogens. The aerobic...
The objective of this study was to determine the effects of processing on sodium - potassium and calcium - phosphorus ratios in tuna canned in oil and in water, peanut butter, white and whole wheat flours. Mineral levels were determined by atomic absorption spectrophotometry in food samples at various stages...
Aerobic heterotrophic bacteria associated with oyster larvae feed
algae Isochrysis galbana, Monochrysis lutheri and Pseudoisochrysis
paradoxa were isolated and enumerated. The bacterial numbers ranged
from 7.8x10³ to 3.9x10⁶ CFU per ml. The bacteria associated with
Pseudoisochrysis sp. were identified and the majority of isolates
belonged to genera Leucothrix (51%). Also...
Absorption of iron from foods has been reported to be inhibited by
various factors including some diet components. Fiber from different
sources has been observed to bind ferrous iron. This may be the reason
for iron deficiencies observed in populations that consume diets high in
non-digestible materials.
The effect of...
Ultrafiltration of d'Anjou pear juice in the batch mode with a
Romicon HFXS MXII pilot-scale ultrafilter produced a "sparkling clear",
light-colored permeate which was sterile, essentially free of pectin,
and lower in phenolics than the feed juice. Retention characteristics
of PM-50, PM-30 and PM-10 hollow fiber membranes were tested at...
The collagen content and composition of collagens in
different age classes of shrimp were determined. Their
physical and chemical characteristics were investigated.
The interrelationship of shrimp size and muscle collagen
content to raw and cooked meat yield was established.
Total collagen content for three lots of round shrimp
with weights...
The effect of entrapped air content on heat penetration
characteristics in institutional size retortable pouches
packed with pears and green beans was investigated. In
addition, products processed in pouches and in number 10
cans were compared using several indices of quality (color,
texture, total phenolics, ascorbic acid) and sensory panel...